Low-temperature resistance of higher plants is significantly enhanced by a nonspecific cyanobacterial desaturase

Ishizaki-Nishizawa, O.; Fujii, Toshio; Azuma, Mizue; Sekiguchi, Keiko; Murata, Norio; Ohtani, Takeshi; Toguri, Toshihiro

doi:10.1038/nbt0896-1003

Cited by 151 publications

(82 citation statements)

References 17 publications

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“…Indeed, when the corresponding gene was overexpressed in the nucleus of transgenic tobacco plants and the protein imported into the plastid, a general increase of 16:1 (t3 and c9) and 18:1, and a concomitant reduction of 16:0, 18:0 and 18:3 fatty acids were observed in leaves. Interestingly, although the overall 16:3 fatty acid level did not change, a significant increase for this fatty acid was observed in the MGDG fraction (Ishizaki-Nishizawa et al 1996).…”

Section: Discussionmentioning

confidence: 80%

“…Unlike the soluble ACP D 9 desaturase which shows only minor specificity towards free 16:0 palmitic acid (1% of that towards 18:0 stearic acid) (McKeon and Stumpf 1982), the acyl-lipid D 9 enzyme used in this study has the ability to desaturate both lipid-linked 16:0 and 18:0 fatty acids (Murata and Wada 1995;Ishizaki-Nishizawa et al 1996). Indeed, when the corresponding gene was overexpressed in the nucleus of transgenic tobacco plants and the protein imported into the plastid, a general increase of 16:1 (t3 and c9) and 18:1, and a concomitant reduction of 16:0, 18:0 and 18:3 fatty acids were observed in leaves.…”

Section: Discussionmentioning

confidence: 99%

“…the stability of cellular membranes and the functionality of photosystem II (PSII), respectively. Nevertheless, they have been largely used to screen genotypes for cold tolerance (Flint et al 1967;Ishizaki-Nishizawa et al 1996). Indeed, the transplastomic plants containing either D 9 desaturase gene showed a slight increase over the control plants as concerns membrane stability following chilling stress.…”

Section: Discussionmentioning

confidence: 99%

“…In order to verify the possibility of manipulating the unsaturation level of fatty acids via plastid transformation, we introduced two D 9 desaturase genes, derived either from the wild potato species Solanum commersonii (De Palma et al 2008) or the cyanobacterium Anacystis nidulans (Ishizaki-Nishizawa et al 1996), into the tobacco plastid genome. The former gene controls the conversion of ACP-stearic acid (18:0) into ) in the plastid (Somerville et al 2000), while the latter is responsible for the production of oleic and palmitoleic (16 : 1 D 9 ) acids from lipid-linked stearic and palmitic (16:0) acids, respectively, in the cyanobacterial cell (Ishizaki-Nishizawa et al 1996).…”

Section: Introductionmentioning

confidence: 99%

“…The former gene controls the conversion of ACP-stearic acid (18:0) into ) in the plastid (Somerville et al 2000), while the latter is responsible for the production of oleic and palmitoleic (16 : 1 D 9 ) acids from lipid-linked stearic and palmitic (16:0) acids, respectively, in the cyanobacterial cell (Ishizaki-Nishizawa et al 1996). Since the unsaturation level of plant membranes can affect cold tolerance (Somerville et al 2000), we also tested the response of transplastomic genotypes to low temperature.…”

Section: Introductionmentioning

confidence: 99%

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Transplastomic tobacco plants expressing a fatty acid desaturase gene exhibit altered fatty acid profiles and improved cold tolerance

Craig¹,

Lenzi²,

Scotti³

et al. 2008

Transgenic Res

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The possibility of altering the unsaturation level of fatty acids in plant lipids by genetic transformation has implications for the stress tolerance of higher plants as well as for their nutritional value and industrial utilisation. While the integration and expression of transgenes in the plastome has several potential advantages over nuclear transformation, very few attempts have been made to manipulate fatty acid biosynthesis using plastid transformation. We produced transplastomic tobacco plants that express a D 9 desaturase gene from either the wild potato species Solanum commersonii or the cyanobacterium Anacystis nidulans, using PEG-mediated DNA uptake by protoplasts. Incorporation of chloroplast antibioticinsensitive point mutations in the transforming DNA was used to select transformants. The presence of the transcript and the D 9 desaturase protein in transplastomic plants was confirmed by northern and western blot analyses. In comparison with control plants, transplastomic plants showed altered fatty acid profiles and an increase in their unsaturation level both in leaves and seeds. The two transgenes produced comparable results. The results obtained demonstrate the feasibility of using plastid transformation to engineer lipid metabolic pathways in both vegetative and reproductive tissues and suggest an increase of cold tolerance in transplastomic plants showing altered leaf fatty acid profiles. This is the first example of transplastomic plants expressing an agronomically relevant gene produced with the ''binding-type'' vectors, which do not contain a heterologous marker gene. In fact, the transplastomic plants expressing the S. commersonii gene contain only plant-derived sequences, a clear attraction from a public acceptability perspective.

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Section: Discussionmentioning

confidence: 80%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Transplastomic tobacco plants expressing a fatty acid desaturase gene exhibit altered fatty acid profiles and improved cold tolerance

Craig¹,

Lenzi²,

Scotti³

et al. 2008

Transgenic Res

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show abstract

Cold shock and adaptation

1998

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Adaptation to environmental stresses, such as temperature fluctuation, is essential for the survival of all living organisms. Cellular responses in both prokaryotes and eukaryotes to high temperature include the synthesis of a set of highly conserved proteins known as the heat shock proteins. In contrast to the heat shock response, adaptation to low temperatures has not been as extensively studied. However, a family of cold‐inducible proteins is evident in prokaryotes. In addition, most organisms have developed adaptive mechanisms that alter both membrane fluidity and the protein translation machinery at low temperature. This review addresses the different adaptive mechanisms used by a variety of organisms with a focus on the molecular mechanisms of cold adaptation that have recently been identified during the cold shock response in Escherichia coli. BioEssays 20:49–57, 1998. © 1998 John Wiley & Sons, Inc.

show abstract

Cold shock and adaptation

1998

View full text Add to dashboard Cite

Adaptation to environmental stresses, such as temperature fluctuation, is essential for the survival of all living organisms. Cellular responses in both prokaryotes and eukaryotes to high temperature include the synthesis of a set of highly conserved proteins known as the heat shock proteins. In contrast to the heat shock response, adaptation to low temperatures has not been as extensively studied. However, a family of cold-inducible proteins is evident in prokaryotes. In addition, most organisms have developed adaptive mechanisms that alter both membrane fluidity and the protein translation machinery at low temperature. This review addresses the different adaptive mechanisms used by a variety of organisms with a focus on the molecular mechanisms of cold adaptation that have recently been identified during the cold shock response in Escherichia coli.

show abstract

Low-temperature resistance of higher plants is significantly enhanced by a nonspecific cyanobacterial desaturase

Cited by 151 publications

References 17 publications

Transplastomic tobacco plants expressing a fatty acid desaturase gene exhibit altered fatty acid profiles and improved cold tolerance

Transplastomic tobacco plants expressing a fatty acid desaturase gene exhibit altered fatty acid profiles and improved cold tolerance

Cold shock and adaptation

Cold shock and adaptation

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