Cold shock and adaptation

Thieringer, Heather A.; Jones, Paula G.; Inouye, Masayori

doi:10.1002/(sici)1521-1878(199801)20:1<49::aid-bies8>3.0.co;2-n

Cited by 337 publications

(216 citation statements)

References 71 publications

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“…NRC-1 contains only one copy each (cspD1 and cspD2). In general, CSPs are involved in various cellular processes and they are believed to enable cells to adapt to low temperature (Thieringer et al 1998). They can bind to single-stranded DNA and RNA and have been suggested to function as RNA chaperones facilitating the initiation of translation under optimal and low temperatures.…”

Section: Bioinformatic Analysismentioning

confidence: 99%

Terrestrial models for extraterrestrial life: methanogens and halophiles at Martian temperatures

Reid¹,

Sparks²,

Lubow³

et al. 2006

International Journal of Astrobiology

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: Cold environments are common throughout the Galaxy. We are conducting a series of experiments designed to probe the low-temperature limits for growth in selected methanogenic and halophilic Archaea. This paper presents initial results for two mesophiles, a methanogen, Methanosarcina acetivorans, and a halophile, Halobacterium sp. NRC-1, and for two Antarctic coldadapted Archaea, a methanogen, Methanococcoides burtonii, and a halophile, Halorubrum lacusprofundi. Neither mesophile is active at temperatures below 5 xC, but both cold-adapted microorganisms show significant growth at sub-zero temperatures (x2 xC and x1 xC, respectively), extending previous lowtemperature limits for both species by 4-5 xC. At low temperatures, both H. lacusprofundi and M. burtonii form multicellular aggregates, which appear to be embedded in extracellular polymeric substances. This is the first detection of this phenomenon in Antarctic species of Archaea at cold temperatures. The low-temperature limits for both psychrophilic species fall within the temperature range experienced on present-day Mars and could permit survival and growth, particularly in subsurface environments. We also discuss the results of our experiments in the context of known exoplanet systems, several of which include planets that intersect the Habitable Zone. In most cases, those planets follow orbits with significant eccentricity, leading to substantial temperature excursions. However, a handful of the known gas giant exoplanets could potentially harbour habitable terrestrial moons.

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Section: Bioinformatic Analysismentioning

confidence: 99%

Terrestrial models for extraterrestrial life: methanogens and halophiles at Martian temperatures

Reid¹,

Sparks²,

Lubow³

et al. 2006

International Journal of Astrobiology

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“…In prokaryotes, a similar acclimation process termed the "cold shock response" under low temperatures (18) and has been extensively characterized in Escherichia coli (19). CspA, the major cold shock protein of E. coli, accounts for more than 10% of total protein synthesis during the cold acclimation phase (20).…”

mentioning

confidence: 99%

A Cold-regulated Nucleic Acid-binding Protein of Winter Wheat Shares a Domain with Bacterial Cold Shock Proteins

Karlson

Nakaminami

Toyomasu

et al. 2002

Journal of Biological Chemistry

140

150

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The molecular mechanisms of cold acclimation are still largely unknown; however, it has been established that overwintering plants such as winter wheat increases freeze tolerance during cold treatments. In prokaryotes, cold shock proteins are induced by temperature downshifts and have been proposed to function as RNA chaperones. A wheat cDNA encoding a putative nucleic acid-binding protein, WCSP1, was isolated and found to be homologous to the predominant CspA of Escherichia coli. The putative WCSP1 protein contains a three-domain structure consisting of an N-terminal cold shock domain with two internal conserved consensus RNA binding domains and an internal glycine-rich region, which is interspersed with three C-terminal CX 2 CX 4 HX 4 C (CCHC) zinc fingers. Each domain has been described independently within several nucleotide-binding proteins. Northern and Western blot analyses showed that WCSP1 mRNA and protein levels steadily increased during cold acclimation, respectively. WCSP1 induction was cold-specific because neither abscisic acid treatment, drought, salinity, nor heat stress induced WCSP1 expression. Nucleotide binding assays determined that WCSP1 binds ssDNA, dsDNA, and RNA homopolymers. The capacity to bind dsDNA was nearly eliminated in a mutant protein lacking C-terminal zinc fingers. Structural and expression similarities to E. coli CspA suggest that WCSP1 may be involved in gene regulation during cold acclimation.

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“…Except for their high affinity to single-stranded DNA or RNA, nothing is known with respect to their biological function [3,4]. In spite of the fact that Csp from the hyperthermophilic bacterium Thermotoga maritima (TmCsp) shows 76% sequence homology (61% identity) to Csp from the mesophilic Bacillus subtilis (CspB), its thermal stability (Tm = 87°C) exceeds that of CspB by 35°C [5].…”

Section: Introduction 2 Materials and Methodsmentioning

confidence: 99%

Does the elimination of ion pairs affect the thermal stability of cold shockprotein from the hyperthermophilic bacterium Thermotoga maritima?

1999

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Cold shock proteins (Csps) from mesophiles and thermophiles differ widely in their stabilities, but show close structural similarity. Sequence variations involve mainly charged groups, supporting the view that ion pairs contribute significantly to the free energy of stabilization. Based on the known 3D structure of mesophilic Bacillus subtilis CspB and the modeled structure of hyperthermophilic Csp from Thermotoga maritima (TmCsp), D9 and H61 of TmCsp have been substituted by asparagine to find out whether the elimination of predicted ion pairs has a destabilizing effect. Thermal unfolding experiments show that the D9N mutant is destabilized by 9°C, whereas H61N exhibits unaltered wild type behavior. The results are in agreement with preliminary NMR data which confirm the predicted structure only for the N-terminal ion pair.

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Cold shock and adaptation

Cited by 337 publications

References 71 publications

Terrestrial models for extraterrestrial life: methanogens and halophiles at Martian temperatures

Terrestrial models for extraterrestrial life: methanogens and halophiles at Martian temperatures

A Cold-regulated Nucleic Acid-binding Protein of Winter Wheat Shares a Domain with Bacterial Cold Shock Proteins

Does the elimination of ion pairs affect the thermal stability of cold shockprotein from the hyperthermophilic bacterium Thermotoga maritima?

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