Low molecular weight liquid media development for Lactobacilli producing bacteriocins

Abstract

BACKGROUND Contemporary purification techniques of Lactobacilli bacteriocins include chemical precipitation and separation through solvents to obtain highly potent semi‐purified bacteriocins. These methods are laborious and bacteriocin yields are low. To address this problem a set of new, efficient, cost effective media, was created, containing low molecular weight nutrient sources (LMWM). Using these media future separation and concentration of the desired metabolic products, using ultra‐ and nano‐filtration from the cultured broth was possible. RESULTS The LMWM were made through serial filtration (filters varying in pore size 30 kDa, 4 kDa and 1 kDa MWCO) of a modified optimum liquid medium for Lactobacilli growth. The developed media were tested for bacteriocin production and biomass growth, using three known bacteriocin‐producing Lactobacilli strains, Lactobacillus casei NCIMB 11970, Lactobacillus plantarum NCIMB 8014, Lactobacillus lactis NCIMB 8586. All were successfully grown (µmax 0.16 to 0.18 h−1) on the LMWM and produced a significant amount of bacteriocins in the range 110 to 130 IU mL−1. CONCLUSIONS LMWM do support Lactobacilli growth and bacteriocin production, establishing an alternative to the current production nutrient media. The uptake of the nutrient sources is facilitated as nitrogen sources, which were primarily responsible for growth, were supported in less complex forms. Copyright © 2012 Society of Chemical Industry

Production and Application of Lactobacillus plantarum IBL-2 Bacteriocins as Meat Product Biopreservatives

“…However, the highest inhibitory activity was obtained from the bacteria grown in LWLM. This result is in accordance with a previous study reporting that LMWLM medium enhanced the growth of Lactobacillus and enhanced bacteriocin production (Zacharof and Lovitt, 2012). The use of LWLM was also preferred due to its' feature which allows filtration and filtration is an efficient method to separate bacteriocin from other protein produced during fermentation process.…”

“…Several growth media including, De Man-Rogosa-Sharpe broth (MRSB), modified nutrient broth (NB), Low molecular weight liquid medium (LMWLM) (Zacharof and Lovitt, 2012), and Tyrptone Glucose Yeast (TGY)-Extract were tested in order to obtain the highest LP IBL-2 production. LP IBL-2 in glycerol 20% was inoculated in MRSB medium for two times.…”

Enhancement of the productivity of the potent bacteriocin avicin A and improvement of its stability using nanotechnology approaches

“…Among the possible approaches to overcome the low yield and the high production costs of bacteriocins is to examine the variables affecting bacteriocin production. In this regard, different studies have examined the effects of various media composition and culture conditions on the yield of bacteriocins 9 , 10 . However, most of these studies have adopted conventional methods to maximize the yield, by changing one independent variable at a time while keeping other variables fixed.…”

A PROBIOTIC APPLICATION OF Lactobacillus acidophilus HT1 FOR THE TREATMENT OF SOME SKIN PATHOGENS

“…Thereafter, biomass was collected using centrifuge at 12000 rpm for 15min, and then a known amount of the optimized medium was added to the precipitant. This cells suspension was kept as usable biomass to be mixed later with the formula (33). The formula including biomass, medium with all compound in (Phase A and Phase B) which mixed very well until homogenization.…”