Abstract:In the present study two novel composites of Calcium phosphates (CaP) and Collagen (COL) were synthesized, hydroxyapatite/Collagen (HA/COL) and hydroxyapatite-btricalcium phosphate/COL (HAbTCP/COL). Collagen was extracted from bovine pericardium submitted to enzymatic digestion and purification by ion-exchange chromatography yielding high purity grade type I collagen. Biocomposites of HAP/COL and HAbTCP/COL were produced with a calcium phosphate/COL ratio of 80/20 (wt. (%)) and were characterized by chemical a… Show more
“…3a), the amide I peak, centered at 1656 cm -1 predominantly corresponds to the C=O stretch vibration [31,41]. In additional studies [26,41] the amide I band in a bone spectrum is representative of the collagen content and structure. The amide II, positioned at 1550 cm -1 , corresponds to a combination of the N-H in-plane bend and the C-H stretch vibrations [26].…”
Section: Formation Of C-cps/w Hybridsmentioning
confidence: 96%
“…For the amide III bands there are two bands assigned at 1241 and 1281 cm -1 . The amide III peaks corresponds to a combination of the C-N stretch and the N-H in-plane bends [31,41]. Amide B is centered at 3081 cm -1 [31,41,68].…”
Section: Formation Of C-cps/w Hybridsmentioning
confidence: 99%
“…The amide III peaks corresponds to a combination of the C-N stretch and the N-H in-plane bends [31,41]. Amide B is centered at 3081 cm -1 [31,41,68]. Two additional bands, visible at 1399 and 1340 cm -1 , can be attributed to the presence of COOH and COO -in the spectrum of pure collagen and pure gelatin [69].…”
Section: Formation Of C-cps/w Hybridsmentioning
confidence: 99%
“…CPS/W (Fig. 7b) shows a visibly porous structure, which is a characteristic for silicon containing apatites [41]. When collagen was mixed CPS/W in 75:25 wt.%, SEM images ( Fig.…”
mentioning
confidence: 94%
“…Other authors studied the possibility to produce bioactive materials between biphasic Hydroxyapatite/ Tricalcium Phospate (HA/TCP) ceramics and type I collagen for filling intramedullary cavities remaining after tumor excision [38], dehiscence-type defects in dogs [39], bone tissue regeneration [40] and rebuilding small lesions in bone tissue engineering [41].…”
“…3a), the amide I peak, centered at 1656 cm -1 predominantly corresponds to the C=O stretch vibration [31,41]. In additional studies [26,41] the amide I band in a bone spectrum is representative of the collagen content and structure. The amide II, positioned at 1550 cm -1 , corresponds to a combination of the N-H in-plane bend and the C-H stretch vibrations [26].…”
Section: Formation Of C-cps/w Hybridsmentioning
confidence: 96%
“…For the amide III bands there are two bands assigned at 1241 and 1281 cm -1 . The amide III peaks corresponds to a combination of the C-N stretch and the N-H in-plane bends [31,41]. Amide B is centered at 3081 cm -1 [31,41,68].…”
Section: Formation Of C-cps/w Hybridsmentioning
confidence: 99%
“…The amide III peaks corresponds to a combination of the C-N stretch and the N-H in-plane bends [31,41]. Amide B is centered at 3081 cm -1 [31,41,68]. Two additional bands, visible at 1399 and 1340 cm -1 , can be attributed to the presence of COOH and COO -in the spectrum of pure collagen and pure gelatin [69].…”
Section: Formation Of C-cps/w Hybridsmentioning
confidence: 99%
“…CPS/W (Fig. 7b) shows a visibly porous structure, which is a characteristic for silicon containing apatites [41]. When collagen was mixed CPS/W in 75:25 wt.%, SEM images ( Fig.…”
mentioning
confidence: 94%
“…Other authors studied the possibility to produce bioactive materials between biphasic Hydroxyapatite/ Tricalcium Phospate (HA/TCP) ceramics and type I collagen for filling intramedullary cavities remaining after tumor excision [38], dehiscence-type defects in dogs [39], bone tissue regeneration [40] and rebuilding small lesions in bone tissue engineering [41].…”
Novel bioactive organic-inorganic hybrid materials that can serve as injectable hydrogel systems for bone tissue regeneration were obtained. The silica nanoparticles (SiNP) prepared in situ by the Stöber method were dispersed in collagen, collagen-chitosan or chitosan sols, which were then subsequently crosslinked. Laser scanning confocal microscopy studies, in which fluorescent SiNP were applied, and SEM images indicated that the nanosilica particles were distributed in the whole volume of the hydrogel matrix. In vitro studies on fibroblast cell viability indicated that the hybrid materials are biocompatible. The silica nanoparticles dispersed in the biopolymer matrix had a positive effect on cell viability. Studies on the mineralization process under simulated body fluid (SBF) conditions confirmed the bioactivity of prepared materials. SEM images revealed mineral phase formation in the majority of the hybrid materials developed. EDS analysis indicated that these mineral phases are mainly composed of calcium and phosphorus. The XRD studies confirmed that mineral phases formed during SBF incubation of hybrid materials based on collagen are bone-like apatite minerals. The silica nanoparticles added to the hydrogel at the stage of synthesis induced the occurrence of mineralization. This process occurs not only at the surface of the material but in its entire volume, which is important for the preparation of scaffolds for bone tissue engineering. The ability of these materials to undergo in situ gelation under physiological temperature and their bioactivity as well as biocompatibility make them interesting candidates for bioactive injectable systems.
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