Low-Cost Leukemic Serum Marker Screening Using Large Area Nanohole Arrays on Plastic Substrates

Valsecchi, Chiara; Jones, T.S.; Wang, Chen; Lochbihler, Hans; Menezes, J. W.; Brolo, Alexandre G.

doi:10.1021/acssensors.6b00368

Cited by 17 publications

(18 citation statements)

References 48 publications

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“…More importantly, the flexible platform-based technologies have recently created an exciting avenue in the field of portable POC diagnostic devices owing to their physiochemical properties as well as integrability into miniaturized and multiplexed analytical chips 27 . Kahraman et al .…”

Section: Introductionmentioning

confidence: 99%

Flexible and Tunable 3D Gold Nanocups Platform as Plasmonic Biosensor for Specific Dual LSPR-SERS Immuno-Detection

Focșan

Craciun

Potara

et al. 2017

Sci Rep

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Early medical diagnostic in nanomedicine requires the implementation of innovative nanosensors with highly sensitive, selective, and reliable biomarker detection abilities. In this paper, a dual Localized Surface Plasmon Resonance - Surface Enhanced Raman Scattering (LSPR- SERS) immunosensor based on a flexible three-dimensional (3D) gold (Au) nanocups platform has been implemented for the first time to operate as a relevant “proof-of-concept” for the specific detection of antigen-antibody binding events, using the human IgG - anti-human IgG recognition interaction as a model. Specifically, polydimethylsilane (PDMS) elastomer mold coated with a thin Au film employed for pattern replication of hexagonally close-packed monolayer of polystyrene nanospheres configuration has been employed as plasmonic nanoplatform to convey both SERS and LSPR readout signals, exhibiting both well-defined LSPR response and enhanced 3D electromagnetic field. Synergistic LSPR and SERS sensing use the same reproducible and large-area plasmonic nanoplatform providing complimentary information not only on the presence of anti-human IgG (by LSPR) but also to identify its specific molecular signature by SERS. The development of such smart flexible healthcare nanosensor platforms holds promise for mass production, opening thereby the doors for the next generation of portable point-of-care devices.

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Section: Introductionmentioning

confidence: 99%

Flexible and Tunable 3D Gold Nanocups Platform as Plasmonic Biosensor for Specific Dual LSPR-SERS Immuno-Detection

Focșan

Craciun

Potara

et al. 2017

Sci Rep

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“…2(a) . By varying the incidence angle, that is, the angle between the light incident on the electrode surface and the line perpendicular to the surface, the diameters of the PR nanoholes could be freely varied without the use of a photomask 31 , 38 – 45 . The PR nanohole patterns on the ITO substrate were then used as templates to deposit gold via ECD.…”

Section: Resultsmentioning

confidence: 99%

Electrochemical detection of dopamine using periodic cylindrical gold nanoelectrode arrays

Kim

Kang

Baek

et al. 2018

Sci Rep

129

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Dopamine is a key molecule in neurotransmission and has been known to be responsible for several neurological diseases. Hence, its sensitive and selective detection is important for the early diagnosis of diseases related to abnormal levels of dopamine. In this study, we reported a new cylindrical gold nanoelectrode (CAuNE) platform fabricated via sequential laser interference lithography and electrochemical deposition. Among the fabricated electrodes, CAuNEs with a diameter of 700 nm, 150 s deposited, was found to be the best for electrochemical dopamine detection. According to cyclic voltammetry results, the linear range of the CAuNE-700 nm was 1–100 µM of dopamine with a limit of detection (LOD) of 5.83 µM. Moreover, owing to the homogeneous periodic features of CAuNEs, human neural cells were successfully cultured and maintained for more than 5 days in vitro without the use of any extracellular matrix proteins and dopamine was detectable in the presence of these cells on the electrode. Therefore, we concluded that the developed dopamine sensing platform CAuNE can be used for many applications including early diagnosis of neurological diseases; function tests of dopaminergic neurons derived from various stem cell sources; and toxicity assessments of drugs, chemicals, and nanomaterials on human neuronal cells.

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“…SPR technique takes advantage of the surface plasmon resonance effect in planar and thin metals, by monitoring the changes of the refractive index due to small mass variations at the plasmonic surface. In the last decades, plasmonic sensors based on this technique have emerged to detect exosomes from ovarian cancer [64], leukemia [65], lung cancer [66], tumor-specific CD8 T Cells [67], or spliced isoforms of the Fas gene [68]. Loyez et al designed a 50 nm gold-coated optical fiber sensor functionalized with antibodies to detect the lung cancer biomarker cytokeratin 17 (CK17).…”

Section: Surface Plasmon Resonance (Spr)mentioning

confidence: 99%

Cancer Diagnosis through SERS and Other Related Techniques

Blanco-Formoso

Alvarez-Puebla

2020

IJMS

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Cancer heterogeneity increasingly requires ultrasensitive techniques that allow early diagnosis for personalized treatment. In addition, they should preferably be non-invasive tools that do not damage surrounding tissues or contribute to body toxicity. In this context, liquid biopsy of biological samples such as urine, blood, or saliva represents an ideal approximation of what is happening in real time in the affected tissues. Plasmonic nanoparticles are emerging as an alternative or complement to current diagnostic techniques, being able to detect and quantify novel biomarkers such as specific peptides and proteins, microRNA, circulating tumor DNA and cells, and exosomes. Here, we review the latest ideas focusing on the use of plasmonic nanoparticles in coded and label-free surface-enhanced Raman scattering (SERS) spectroscopy. Moreover, surface plasmon resonance (SPR) spectroscopy, colorimetric assays, dynamic light scattering (DLS) spectroscopy, mass spectrometry or total internal reflection fluorescence (TIRF) microscopy among others are briefly examined in order to highlight the potential and versatility of plasmonics.

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Low-Cost Leukemic Serum Marker Screening Using Large Area Nanohole Arrays on Plastic Substrates

Cited by 17 publications

References 48 publications

Flexible and Tunable 3D Gold Nanocups Platform as Plasmonic Biosensor for Specific Dual LSPR-SERS Immuno-Detection

Flexible and Tunable 3D Gold Nanocups Platform as Plasmonic Biosensor for Specific Dual LSPR-SERS Immuno-Detection

Electrochemical detection of dopamine using periodic cylindrical gold nanoelectrode arrays

Cancer Diagnosis through SERS and Other Related Techniques

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