Electrochemical detection of dopamine using periodic cylindrical gold nanoelectrode arrays

Kim, Da-Seul; Kang, Ee-Seul; Baek, Seungho; Choo, Sung-Sik; Chung, Yong-Ho; Lee, Dong-Hyun; Min, Junhong; Kim, Tae-Hyung

doi:10.1038/s41598-018-32477-0

Cited by 136 publications

(75 citation statements)

References 53 publications

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“…Boron-doped carbon nanotubes could be used to determine levels of dopamine and ephedrine in the presence of urate [12]. Composites of graphene oxide with Bi 2 S 3 nanorods were used for the electrochemical determination of dopamine [13], and arrays of cylindrical gold nanoelectrodes could be used for both the detection of dopamine and for the immobilization and growth of human neural cells [14]. Many other systems based on organic and inorganic materials have been reported for the sensing of dopamine [2,3].Synthetic bio-receptors such as aptamers appeared to be highly attractive for selective detection of dopamine [15][16][17].…”

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confidence: 99%

Electrochemical Aptasensor for Detection of Dopamine

et al. 2020

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This work presents a proof of concept of a novel, simple, and sensitive method of detection of dopamine, a neurotransmitter within the human brain. We propose a simple electrochemical method for the detection of dopamine using a dopamine-specific aptamer labeled with an electrochemically active ferrocene tag. Aptamers immobilized on the surface of gold screen-printed gold electrodes via thiol groups can change their secondary structure by wrapping around the target molecule. As a result, the ferrocene labels move closer to the electrode surface and subsequently increase the electron transfer. The cyclic voltammograms and impedance spectra recorded on electrodes in buffer solutions containing different concentration of dopamine showed, respectively, the increase in both the anodic and cathodic currents and decrease in the double layer resistance upon increasing the concentration of dopamine from 0.1 to 10 nM L −1 . The high affinity of aptamer-dopamine binding (KD ≈ 5 nM) was found by the analysis of the binding kinetics. The occurrence of aptamer-dopamine binding was directly confirmed with spectroscopic ellipsometry measurements.Chemosensors 2020, 8, 28 2 of 11 done on the electrochemical detection of dopamine and other neurotransmitters [2,3]. Dopamine can be very easily detected in an aqueous solution by electrochemical methods such as cyclic voltammetry since dopamine undergoes electrochemical oxidation. However, a major issue is the selectivity since complex matrices such as blood contain a range of other oxidizable compounds such as urate or ascorbate which would also generate signals, leading to inaccurate readings. Selectivity towards dopamine is therefore required.A wide range of electrode materials have been proposed to increase the selectivity of dopamine detection [2,3]. A few recent examples include the use of materials such as Nafion combined with graphite [4] or multi-walled carbon nanotubes [5] to improve the selectivity towards dopamine. Other researchers have utilized graphene-modified screen-printed electrodes [6] or graphene aerogels [7] as substrates for the selective determination of dopamine. Composites of carbon nanotubes and graphene oxide could be used to determine dopamine, nitrate, ascorbate, and urate [8]. Metal nanoparticles have also been utilized, for example palladium nanoparticles combined with graphene [9], which were used as a base of electrochemical determination of dopamine whereas a gold nanoparticle/DNA/polymer composite could be used for the simultaneous determination of dopamine, uric acid, guanine, and adenine [10]. Composites of gold nanoparticles with conducting polymers have also been used for the selective determination of dopamine [11]. Boron-doped carbon nanotubes could be used to determine levels of dopamine and ephedrine in the presence of urate [12]. Composites of graphene oxide with Bi 2 S 3 nanorods were used for the electrochemical determination of dopamine [13], and arrays of cylindrical gold nanoelectrodes could be used for both the detection of dopamine an...

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confidence: 99%

Electrochemical Aptasensor for Detection of Dopamine

et al. 2020

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“…As previously mentioned, HNPAs are capable of improving cell adhesion and growth through the reorganization of the cytoskeleton. Therefore, in this study, we affirm that HNPAs are powerful for accelerating stem cell growth and differentiation [53,63,64]. According to this premise, we determined the correlation of cell adhesion, spreading, and growth with neural differentiation of mNSCs.…”

Section: The Effects Of Nanohole Patterns On Mnscs Adhesion and Growthmentioning

confidence: 55%

Enhancing Neurogenesis of Neural Stem Cells Using Homogeneous Nanohole Pattern-Modified Conductive Platform

Cho

Kim

Suhito

et al. 2019

IJMS

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Biocompatible platforms, wherein cells attach and grow, are important for controlling cytoskeletal dynamics and steering stem cell functions, including differentiation. Among various components, membrane integrins play a key role in focal adhesion of cells (18–20 nm in size) and are, thus, highly sensitive to the nanotopographical features of underlying substrates. Hence, it is necessary to develop a platform/technique that can provide high flexibility in controlling nanostructure sizes. We report a platform modified with homogeneous nanohole patterns, effective in guiding neurogenesis of mouse neural stem cells (mNSCs). Sizes of nanoholes were easily generated and varied using laser interference lithography (LIL), by changing the incident angles of light interference on substrates. Among three different nanohole patterns fabricated on conductive transparent electrodes, 500 nm-sized nanoholes showed the best performance for cell adhesion and spreading, based on F-actin and lamellipodia/filopodia expression. Enhanced biocompatibility and cell adhesion of these nanohole patterns ultimately resulted in the enhanced neurogenesis of mNSCs, based on the mRNAs expression level of the mNSCs marker and several neuronal markers. Therefore, platforms modified with homogeneous nanohole patterns fabricated by LIL are promising for the precise tuning of nanostructures in tissue culture platforms and useful for controlling various differentiation lineages of stem cells.

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“…In particular, electrochemical biosensors based on electrodes modified with Ag NPs showed high selectivity and sensitivity . Finally, a new cylindrical gold nanoelectrode platform manufactured by sequential lithography of laser interference was discovered, which proved to be the best option for electrochemical detection of dopamine . From all these electrochemical results, we may observe that the study of the interactions between dopamine and noble metal surfaces, especially Ag, can be considered a key factor to achieve more efficient electrochemical sensor materials.…”

Section: Introductionmentioning

confidence: 75%

Electric Field Effects on the Adsorption of Dopamine Species on Ag(111): DFT Investigation of Interaction Mechanism

et al. 2020

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The study of interactions between dopamine (DA) and noble metal surfaces as Ag, is envisaged as a fundamental factor to attain more efficient electrochemical sensor materials. We describe, in the framework of Density Functional Theory, the adsorption of four dopamine species, neutral (NDA), zwitterionic (ZDA), protonated (PDA) and deprotonated (DPDA) species on Ag (111), upon the application of an external electric field (E), a fact of pertinence for electrochemistry tests. The ZDA and PDA species show relatively large binding energies of about 2.9 eV to 4.6 eV and suffer a significant flatting upon adsorption, compared with DPDA and NDA. The adsorbates binding energy is an increasing function of the applied E for ZDA and PDA species, showing a great change when the results for positive and negative E are compared. An electron transfer is produced from NDA, ZDA and PDA species to Ag, while in the case of DPDA an opposite behavior is obtained. As the sign of E is modified, an increase of charge values in all DA species is observed. The theoretical results obtained here show that ZDA and PDA species are the most affected DA species upon adsorption on Ag(111) and when non-zero electric fields are applied.

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Electrochemical detection of dopamine using periodic cylindrical gold nanoelectrode arrays

Cited by 136 publications

References 53 publications

Electrochemical Aptasensor for Detection of Dopamine

Electrochemical Aptasensor for Detection of Dopamine

Enhancing Neurogenesis of Neural Stem Cells Using Homogeneous Nanohole Pattern-Modified Conductive Platform

Electric Field Effects on the Adsorption of Dopamine Species on Ag(111): DFT Investigation of Interaction Mechanism

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