Low-Carbohydrate and High-Fat Diets on the Promotion of Hepatic Steatosis in Rats

Leonardi, Daphne Santoro; Feres, Maria Beatriz de Camargo; Portari, Guilherme Vannucchi; Zanuto, M. E.; Zucoloto, Sérgio; Jordão, Alceu Afonso

doi:10.1055/s-0030-1255021

Cited by 16 publications

(13 citation statements)

References 34 publications

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“…The excessive consumption of fat was also responsible for the increased glucose in the HF and HPF groups, in agreement with previous studies [42]. Fructosamine is a marker of the levels of glycated serum protein and the levels were smaller in the HF and HPF groups compared with the control group, as the serum fructosamine levels directly reflect the glucose intake by the diet, because the fructosamine concentration may reflect the plasma concentrations of glucose over the last 20 days [43].…”

Section: Discussionsupporting

confidence: 91%

Metabolic Differences in the Steatosis Induced by a High-Fat Diet and High-Protein-Fat Diet in Rats

Leonardi-Carvalho¹,

Zucoloto²,

Ovídio³

et al. 2015

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Abstract:The study aim was to evaluate some effects of a high-fat diet and a high-protein-fat diet in the hepatic steatosis development in rats. Twenty nine Wistar rats were divided at random into three groups: Control: a control diet with soybean oil; HF: a high-fat diet with 50% of lard; HPF: a high-protein-fat diet with about 40% of protein and 50% of lard. The groups were fed with these diets during four weeks. The following parameters were analyzed: hepatic steatosis, serum and hepatic lipid profile, lipid peroxidation, antioxidants concentration and hepatocytes damage. The HF group showed the highest caloric intake per day and the highest weight gain (p<0.05). The hepatic cholesterol concentration was highest in the HF group and the serum total cholesterol concentration was highest in the HPF group (p<0.05). The macrovesicular steatosis was predominant in the HF group, with ballooning hepatocytes and Mallory bodies and in the HPF group predominant microvesicular steatosis was found, without ballooning hepatocytes and Mallory bodies. An increase in TBARS and a decrease in Vitamin E in the HF and HPF groups (p<0.05) was also found. The HF group showed the highest acid oleic deposit in the liver, followed by the HPF group. The SFA hepatic concentration was similar among the groups (p>0.05), whereas the MUFA concentration was higher in the HF and HPF (p>0.05) than the control group (p<0.05). Therefore, the experimental model used is an efficient model to study hepatic steatosis. The HF and HPF groups had the same behaviors for oxidative stress, serum glucose and hepatic damage in responses to the experimental diets, but these groups showed different esteatosis features.

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Section: Discussionsupporting

confidence: 91%

Metabolic Differences in the Steatosis Induced by a High-Fat Diet and High-Protein-Fat Diet in Rats

Leonardi-Carvalho¹,

Zucoloto²,

Ovídio³

et al. 2015

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“…The offer of high-fat diets to rats represents an experimental model for the study of diseases such as NAFLD and metabolic syndrome [20][21][22] . The effects of trans fatty acids on the increase in LDL cholesterol levels and the decrease of HDL cholesterol levels are also well known 23 .…”

Section: Discussionmentioning

confidence: 99%

Oxidative stress and fatty acid profile in Wistar rats subjected to acute food restriction and refeeding with high-fat diets

Nassar

Marot

Ovídio³

et al. 2014

Acta Cir. Bras.

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“…At the end of 30 days, the two groups were sacrificed by decapitation and blood samples were collected for the determination of total serum cholesterol, serum protein and glycemia. The liver was then weighed and placed in liquid nitrogen (-196°C) for later determination of hepatic fat by by Bligh and Dyer extraction 19 . Glycemia, total cholesterol and protein were determined by a colorimetric enzymatic method using a commercial kit (Labtest®, Minas Gerais, Brasil).…”

Section: Methodsmentioning

confidence: 99%

A high-fat diet as a model of fatty liver disease in rats

Picchi¹,

Mattos²,

Barbosa³

et al. 2011

Acta Cir. Bras.

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PURPOSE:The objective of the present study was to analyze the physiological and metabolic changes occurring in rats subjected to high-fat diet for one month. METHODS:The animals received a modified AIN-93 diet with increased lipid content and decreased carbohydrate content, while the control group received the normal AIN-93 diet. RESULTS: It was observed that the high-fat diet did not induce weight gain but led to greater gain of hepatic fat compared to control. Biochemcal parameters, glycemia, total cholesterol and serum protein did not differ between groups. In parallel, rats receiving the high-fat diet consumed less feed. CONCLUSION: The development of obesity through high-fat diet is associated with increased energy intake and time of exposure to the diet, while the metabolic syndrome is more associated with the combination of a diet rich in fat and carbohydrates. Key words: Obesity. Lipids. Hyperphagia. Weight Gain. Rats. RESUMO OBJETIVO:Analisar modificações fisiológicas e metabólicas em ratos submetidos à dieta hiperlipídica por um mês. MÉTODOS: Os animais receberam a dieta AIN-93 modificada, com aumento do teor de lipídeos e diminuição do teor de carboidratos, enquanto o grupo controle recebeu a dieta AIN-93. RESULTADOS: Foi observado que a dieta hiperlipídica não induziu o ganho de peso, porém levou a um maior ganho de gordura hepática, em comparação ao grupo controle. Os parâmetros bioquímicos, glicemia, colesterol total e proteína sérica não diferiram entre os grupos. Ao mesmo tempo os ratos alimentados com dieta hiperlipídica, apresentaram uma menor ingestão de alimentos. CONCLUSÃO: O desenvolvimento da obesidade com a dieta hiperlipídica está associado com aumento da ingestão de energia e tempo de exposição à dieta, enquanto a síndrome metabólica está mais associada a dietas com alto teor de gordura e carboidratos, concomitantemente. Descritores: Obesidade. Lipídeos. Hiperfagia. Ganho de peso. Ratos. Picchi MG et al.

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Low-Carbohydrate and High-Fat Diets on the Promotion of Hepatic Steatosis in Rats

Abstract: the fat increase in the diet promoted increase of the oxidative stress, evidenced by the decrease in the hepatic concentration of vitamin E, showing its antioxidant role against the probable generated free radicals, the ones which possibly exercised a role in the steatosis occurrence.

Cited by 16 publications

References 34 publications

Metabolic Differences in the Steatosis Induced by a High-Fat Diet and High-Protein-Fat Diet in Rats

Metabolic Differences in the Steatosis Induced by a High-Fat Diet and High-Protein-Fat Diet in Rats

Oxidative stress and fatty acid profile in Wistar rats subjected to acute food restriction and refeeding with high-fat diets

A high-fat diet as a model of fatty liver disease in rats

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