A high-fat diet as a model of fatty liver disease in rats

Abstract

PURPOSE:The objective of the present study was to analyze the physiological and metabolic changes occurring in rats subjected to high-fat diet for one month. METHODS:The animals received a modified AIN-93 diet with increased lipid content and decreased carbohydrate content, while the control group received the normal AIN-93 diet. RESULTS: It was observed that the high-fat diet did not induce weight gain but led to greater gain of hepatic fat compared to control. Biochemcal parameters, glycemia, total cholesterol and serum protein did not differ between groups. In parallel, rats receiving the high-fat diet consumed less feed. CONCLUSION: The development of obesity through high-fat diet is associated with increased energy intake and time of exposure to the diet, while the metabolic syndrome is more associated with the combination of a diet rich in fat and carbohydrates. Key words: Obesity. Lipids. Hyperphagia. Weight Gain. Rats. RESUMO OBJETIVO:Analisar modificações fisiológicas e metabólicas em ratos submetidos à dieta hiperlipídica por um mês. MÉTODOS: Os animais receberam a dieta AIN-93 modificada, com aumento do teor de lipídeos e diminuição do teor de carboidratos, enquanto o grupo controle recebeu a dieta AIN-93. RESULTADOS: Foi observado que a dieta hiperlipídica não induziu o ganho de peso, porém levou a um maior ganho de gordura hepática, em comparação ao grupo controle. Os parâmetros bioquímicos, glicemia, colesterol total e proteína sérica não diferiram entre os grupos. Ao mesmo tempo os ratos alimentados com dieta hiperlipídica, apresentaram uma menor ingestão de alimentos. CONCLUSÃO: O desenvolvimento da obesidade com a dieta hiperlipídica está associado com aumento da ingestão de energia e tempo de exposição à dieta, enquanto a síndrome metabólica está mais associada a dietas com alto teor de gordura e carboidratos, concomitantemente. Descritores: Obesidade. Lipídeos. Hiperfagia. Ganho de peso. Ratos. Picchi MG et al.

Evaluation of ovarian and metabolic effects of GnRH modulators in two rat models of polycystic ovary syndrome

“…Though food intake was lower in HFD-fed rats and overall calorie intake seemed to be similar in both groups. Other researchers also reported reduced food intake in HFD-fed animals (Chen, Ho, Lee, Lee, & Pan, 2008;Picchi et al, 2011). Both leuprolide and cetrorelix had no effect on food intake and body weight in letrozole-administered rats as well as HFD-fed rats.…”

Comparative Effects and Mechanisms of Chitosan and Its Derivatives on Hypercholesterolemia in High-Fat Diet-Fed Rats

“…Moreover, there are no significant difference in intestinal weights among groups ( Figure 1D). Picchi et al (2011) have shown that HF diet-administered rats do not experience elevated weight gain but do develop a fatty liver compared to the rats that received the normal AIN-93 diet, which is similar to our result that the liver weight significantly increases without a significant increase in body weight gain in HF diet-fed rats [14]. Interestingly, the results show that the supplementation of 5% CO does not exhibit the therapeutic potential in HF diet-induced liver weight gains but causes a significant reduction in body weight, which was also found in diabetic Goto-Kakizaki rats with the supplementation of CO by Teodoro et al (2016) [15], implying that a 5% CO supplementation may exert the liver damage potential.…”

A gut reaction: the combined influence of exercise and diet on gastrointestinal microbiota in rats

“…The initial weight loss with the high‐fat diet on body weight has been reported in several rat obesity studies (Picchi et al . ; van Waveren et al . ).…”

“…The initial weight loss on HF diet is in line with previous rodent studies suggesting diets high in fat and carbohydrates may naturally predispose rats to reduce food intake in order to maintain a stable body weight or fat composition (Picchi et al . ; Tillman et al . ; Crescenzo et al .…”