Loop‐Mediated Isothermal Amplification as a Fast Noninvasive Method of <i>Helicobacter pylori</i> Diagnosis

Yari, Farideh; Abiri, Ramin; Aryan, Ehsan; Jouybari, Touraj Ahmadi; Navabi, Jafar; Alvandi, Amirhooshang

doi:10.1002/jcla.21880

Cited by 25 publications

(19 citation statements)

References 47 publications

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“…They tested it on 1007 stool specimens using culture and RUT as gold standards. The rate of positivity was 82%, that is much higher than the RUT (53%) and culture (44%) and also higher than the stool antigen test (SAT) (58%) …”

Section: Invasive Testsmentioning

confidence: 79%

“…The rate of positivity was 82%, that is much higher than the RUT (53%) and culture (44%) and also higher than the stool antigen test (SAT) (58%). 34 A droplet-digital PCR (ddPCR) using 16S rDNA as the target gene was developed by Talarico et al 35 After this first step, their goal was to develop a ddPCR targeting cagA and able to identify its EPIYA phosphorylation motifs, directly from stools. The test allowed to detect Molecular targeting methods can be applied on colonies grown in culture, such as Random Amplified Polymorphic DNA (RAPD) to compare isolates from parents and their children 39 or to detect multiple H. pylori infection, for example 48% of the cases in Tunisia but only 5% in France.…”

Section: Molecular Methodsmentioning

confidence: 99%

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Diagnostic of Helicobacter pylori infection

et al. 2016

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There is progress in endoscopy techniques. While it is not yet possible to detect Helicobacter pylori directly in the stomach, it becomes easier to detect the mucosal changes induced by the bacteria. Some small changes can also increase the sensitivity of the invasive tests, for example culture or histology, but the wide use of proton-pump inhibitors has a negative impact on these tests. Only molecular methods are able to detect a limited load of bacteria, especially by using real-time PCR but also with new methods, for example dual-priming oligonucleotide-based PCR, loop-medicated isothermal amplification, droplet-digital PCR or a multiple genetic analysis system. Among the noninvasive tests, urea breath test remains a test of major interest, while there are attempts to develop an ammonia breath test and other nanosensor devices. A new antigen stool test, a chemoluminescence immunoassay using the LIAISON apparatus has also been tested for the first time with success. Despite its limitations, serology remains the most popular test to detect H. pylori antibodies. It also allows pepsinogen dosage which is of interest for detecting atrophy.

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Section: Invasive Testsmentioning

confidence: 79%

Section: Molecular Methodsmentioning

confidence: 99%

Diagnostic of Helicobacter pylori infection

et al. 2016

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“…The proportion of gastric cancer incidence varies regionally. South America, Eastern Europe, and especially eastern Asia are the most susceptible regions (Yari et al, 2016). Globally, most gastric cancer patients are unresectable when diagnosed (Chen, Li, Zhao, Xiao, & Guo, 2017).…”

Section: Introductionmentioning

confidence: 99%

Identification and functional annotation of metabolism‐associated lncRNAs and their related protein‐coding genes in gastric cancer

Xie

et al. 2018

Molec Gen & Gen Med

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BackgroundLong noncoding RNAs (lncRNAs) play important roles in carcinogenesis. However, the roles of metabolism‐associated lncRNAs in cancers are still unclear.MethodsA microarray of metabolism‐associated lncRNAs was used to detect their expression patterns between gastric cancer and paired nontumorous tissues. Its results and gastric cancer differential gene expression data from public databases were used to screen the metabolic pathway‐associated lncRNAs. A metabolic network with microRNAs (miRNAs), lncRNAs, and protein‐coding genes was further constructed. Finally, the expression of TOPORS antisense RNA 1 (TOPORS‐AS1), a screened highly expressed lncRNA and its associated protein‐coding gene, NADH: ubiquinone oxidoreductase subunit B6 (NDUFB6), were verified by reverse transcription polymerase chain reaction.ResultsA total of eight upregulated and one downregulated lncRNAs and 25 upregulated and 20 downregulated protein‐coding genes were found to be involved in metabolism in gastric cancer. Within the lncRNAs–miRNAs–mRNAs metabolic network, 78 miRNA‐target links, 546 positive coexpression relationships, and 191 protein–protein interactions were found. The expression of TOPORS‐AS1 and its associated gene, NDUFB6 in gastric cancer tissues was significantly lower than that in adjacent nontumor tissues. Moreover, NDUFB6 expression was associated with the invasion and distal metastasis of gastric cancer.ConclusionsThe metabolism‐associated lncRNAs play important roles in the occurrence of gastric cancer.

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“…Another amplification method, namely the loop‐mediated isothermal amplification (LAMP), targeting ure C, was tested on stool specimens in comparison to SAT after determination of its analytical sensitivity (6 H. pylori genomes/reaction) and showed a high sensitivity …”

Section: Molecular Methodsmentioning

confidence: 99%

Diagnosis of Helicobacter pylori infection

et al. 2017

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Important progress is being made in endoscopic methods which allow clinicians to predict the presence of Helicobacter pylori based on characteristics of gastric mucosa and to obtain targeted biopsies. There are also important developments in molecular methods with various techniques derived from standard PCR, applied both on gastric biopsies and stool specimens. Progress is being made in microfluidic systems to get a reliable diagnosis in a very short time. The interest of the C urea breath test has been confirmed as well as stool antigen tests. Attempts are being made to find biological markers of premalignant conditions by serology, other than pepsinogens.

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Loop‐Mediated Isothermal Amplification as a Fast Noninvasive Method of Helicobacter pylori Diagnosis

Abstract: LAMP technique has been characterized by high sensitivity and low detection limit for the detection of H. pylori in stool specimen. Clinical diagnostic performance of LAMP was better than the stool antigen test.

Cited by 25 publications

References 47 publications

Diagnostic of Helicobacter pylori infection

Diagnostic of Helicobacter pylori infection

Identification and functional annotation of metabolism‐associated lncRNAs and their related protein‐coding genes in gastric cancer

Diagnosis of Helicobacter pylori infection

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