Lonicera latent virus, a new carlavirus serologically related to poplar mosaic virus: some properties and inactivation in vivo by heat treatment

Meer, F.A. van der; Maat, D. Z.; Vink, Jochem N.A.

doi:10.1007/bf01974336

Cited by 6 publications

(6 citation statements)

References 7 publications

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“…The data presented below are from tests without evidence of non-specific precipitation (see also Van der Meer et al, 1980). The homologous titres of the FB3 antiserum samples obtained from successive bleedings were from 256-4096, when tested just after harvest.…”

Section: Resultsmentioning

confidence: 99%

“…Virus purification mainly was as with Lonicera latent virus (Van der Meer et al, 1980). Essential differences are indicated below.…”

Section: Methodsmentioning

confidence: 99%

“…An antiserum to a Dutch isolate of PVM with unknown titre (Berg, 1964) Serological testing with the micro-precipitin test, to determine antiserum titres and for virus identification was as described by Van der Meer et al (1980). An antiserum to a Dutch isolate of PVM with unknown titre (Berg, 1964) Serological testing with the micro-precipitin test, to determine antiserum titres and for virus identification was as described by Van der Meer et al (1980).…”

Section: Methodsmentioning

confidence: 99%

“…Electron microscopy was as with Lonicera latent virus, measuring particles from photographic negatives (Van der Meer et al, 1980).…”

Section: Methodsmentioning

confidence: 99%

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Poplar mosaic virus: purification, antiserum preparation, and detection in poplars with the enzyme-linked immunosorbent assay (ELISA) and with infectivity tests on Nicotiana megalosiphon

Meer¹,

Maat²,

Vink³

1980

Netherlands Journal of Plant Pathology

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Poplar mosaic virus (PMV) was purified from Nicotiana megalosiphon or N. clevelandii and antisera with titres from 256 to 4096 were prepared. One of these was used for the detection of PMV in poplar stools with the enzyme-linked immunosorbent assay (ELISA). Although ELISA was less sensitive than the infectivity test on N. megalosiphon, both tests were equally reliable when using lower leaves in July and August, and when applied to tip leaves at the end of the growing season, With both tests more infected trees were detected than with visual inspection. For PMV a particle length of 661 nm was calculated. AddressInstituut voor Plantenziektenkundig Onderzoek, Binnenhaven 12, Postbus 42, 6700 AA Wageningen.

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Section: Resultsmentioning

confidence: 99%

“…Virus purification mainly was as with Lonicera latent virus (Van der Meer et al, 1980). Essential differences are indicated below.…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…Electron microscopy was as with Lonicera latent virus, measuring particles from photographic negatives (Van der Meer et al, 1980).…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Poplar mosaic virus: purification, antiserum preparation, and detection in poplars with the enzyme-linked immunosorbent assay (ELISA) and with infectivity tests on Nicotiana megalosiphon

Meer¹,

Maat²,

Vink³

1980

Netherlands Journal of Plant Pathology

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“…1970) and, particularly after heat treatment, in buds along a branch (Campbell, 1968: Campbell & Best, 1963Trifinov, 1969). Berg (1964) did not recognise symptom variants but van der Meer, Maat & Vink (1980a) were able to select, after passage through N. megalosiphon. 197 1 ) or aphid transmissibility (citrus tristeza: Raccah, Lobenstein & Singer, 1980) our experience hith PMV in cv.…”

Section: Discussionmentioning

confidence: 99%

The distribution of poplar mosaic virus in hybrid poplars and virus detection by ELISA

Cooper

Edwards

1981

Annals of Applied Biology

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S U M M A R YPurified poplar mosaic virus (PMV) at a concentration of 8 ng/ml was readily detected by enzyme-linked immunosorbent assay (ELISA). Bioassay in Nicotiana megalosiphon was more sensitive (detecting 1-4 ng/ml) and latex flocculation less sensitive (c. 25 ng/ml) than ELISA assays. While the foliar sap of fresh, naturally-infected poplars (e.g. Populus x euramericana cv. Robusta) was not infective at dilutions greater than 2 x lop2, ELISA easily detected PMV antigen when sap was diluted 4 x in buffer or when one part of infected tissue was triturated with 99 parts healthy leaf. Furthermore, although sap from poplar leaves stored at -20 OC for 6 months was not infective, PMV was still detectable in ELISA tests. PMV antigen in poplar leaves was not all pelleted after centrifugation for 2.5 h at 130 000 g yet parallel tests using unbuffered sap from systemically infected Nicotiana megalosiphon foliage showed that infectivity was restricted to the pellet. In poplar foliage, the concentration of PMV antigen was generally greatest where symptoms were most obvious; least antigen was detected in the overwintering leaves located at the bases of long shoots. In winter, when root and inner bark tissue in the trunk was an erratic source of PMV, the virus was readily detected in buds, the concentration being greatest in the bases, including the meristem, of terminal buds.Propagation from single node cuttings of P. x euramericana cv. Regenerata allowed the selection of clones that consistently showed either 'severe' or 'mild' foliar symptoms. The associated virus isolates also infected another poplar clone causing symptoms characteristic of their source. ELISA consistently detected less PMV antigen in field-grown cv. Regenerata than in cv. Robusta foliage, but this was reversed when the associated virus isolates were propagated in Nicotiana glutinosa at 24 OC. During 6 yr, 21 out of 127 poplars at a site in Western England, became infected with PMV. By contrast, in Eastern England, none of 46 were infected. The aphids Pterocornma populea and Myzus persicae did not transmit PMV.

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Serology and Immunochemistry

Koenig¹

1988

The Plant Viruses

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Lonicera latent virus, a new carlavirus serologically related to poplar mosaic virus: some properties and inactivation in vivo by heat treatment

Cited by 6 publications

References 7 publications

Poplar mosaic virus: purification, antiserum preparation, and detection in poplars with the enzyme-linked immunosorbent assay (ELISA) and with infectivity tests on Nicotiana megalosiphon

Poplar mosaic virus: purification, antiserum preparation, and detection in poplars with the enzyme-linked immunosorbent assay (ELISA) and with infectivity tests on Nicotiana megalosiphon

The distribution of poplar mosaic virus in hybrid poplars and virus detection by ELISA

Serology and Immunochemistry

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