Poplar mosaic virus: purification, antiserum preparation, and detection in poplars with the enzyme-linked immunosorbent assay (ELISA) and with infectivity tests on Nicotiana megalosiphon

Abstract

Poplar mosaic virus (PMV) was purified from Nicotiana megalosiphon or N. clevelandii and antisera with titres from 256 to 4096 were prepared. One of these was used for the detection of PMV in poplar stools with the enzyme-linked immunosorbent assay (ELISA). Although ELISA was less sensitive than the infectivity test on N. megalosiphon, both tests were equally reliable when using lower leaves in July and August, and when applied to tip leaves at the end of the growing season, With both tests more infected trees were detected than with visual inspection. For PMV a particle length of 661 nm was calculated. AddressInstituut voor Plantenziektenkundig Onderzoek, Binnenhaven 12, Postbus 42, 6700 AA Wageningen.

Lonicera latent virus, a new carlavirus serologically related to poplar mosaic virus: some properties and inactivation in vivo by heat treatment

“…The calculated particles length of 656 nm for the Lonicera virus lies clearly within the range of that of the carlaviruses (Fenner, 1976) and is almost equal to the length of 661 nm which we found for PMV (Van der Meer et al, 1980). Based on particle length and results of serological tests only, the virus could be considered as a strain of PMV.…”

“…They are described elsewhere in more detail (Van der Meer et al, 1980). The type isolate M was isolated from L.japonica cv.…”

The detection of poplar mosaic virus and its occurrence in a range of clones in England and Poland

“…Nevertheless, the concentration of ELISA detectable antigen seemed to vary amongst buds and leaves in differing positions on branches (see also COOPER and EDWARDS 1981) and also, perhaps as a consequence, in leaves of poplars in stool beds (cf. observations by VAN DER MEER et al 1980).…”

“…It was noted early in the investigation that ELISA tests on buds were not always in agreement with foliar symptom expression and in some instances results of ELISA bud tests done on the same clone but on different occasions were contradictory. Knowing that PMV can be unequally distributed in poplars (VAN DER MEER 1980;COOPER and EDWARDS 1981), extensive ELISA tests using buds were done on 24 clones (with and without foliar symptoms) to estimate the number needed to confidently say whether or not a stool contained detectable virus antigen. Two sampling methods were used.…”

“…BERG (1964) used symptom expression in poplar, electron microscopy and infectivity assays in parallel to look for PMV in the only reported extensive survey for the virus. More recently, the enzyme-linked immunosorbent assay has been shown to reliably detect PMV in a few poplar cultivars (VAN DER MEER et al 1980;COOPER and EDWARDS 1981). Here we report the results of a survey in which we compared the reliability of visual (symptomology), ELISA and infectivity tests as criteria for detecting PMV infection in a large range of clones representing the sections Leucoides, Leuce, Aigeiros, Tacamahaca and their intersectional hybrids.…”