Longitudinal monitoring of microglial/macrophage activation in ischemic rat brain using Iba-1-specific nanoparticle-enhanced magnetic resonance imaging

Sillerud, Laurel O.; Yang, Yirong; Yang, Lisa; Duval, Kelsey B; Thompson, Jeffrey; Yang, Yi

doi:10.1177/0271678x20953913

Cited by 19 publications

(14 citation statements)

References 55 publications

(107 reference statements)

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“…This has indeed been demonstrated for microglia or macrophages using Iba-1 (ionized calcium binding adaptor molecule 1) antibody-conjugated superparamagnetic nanoparticles, measured in rats up to 4 weeks after transient MCAO. 115 Conversely, iron oxide particles may be coated with a natural leukocyte membrane to target activated endothelium, demonstrated using neutrophilmimetic magnetic nanoprobes in poststroke mice. 116 Another approach for in vivo imaging of neuroinflammation involves using responsive contrast agents.…”

Section: Imaging Molecular Markers Of Inflammationmentioning

confidence: 99%

Neuroinflammation, Stroke, Blood-Brain Barrier Dysfunction, and Imaging Modalities

Candelario‐Jalil

Dijkhuizen

Magnus

2022

Stroke

268

150

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Maintaining blood-brain barrier (BBB) integrity is crucial for the homeostasis of the central nervous system. Structurally comprising the BBB, brain endothelial cells interact with pericytes, astrocytes, neurons, microglia, and perivascular macrophages in the neurovascular unit. Brain ischemia unleashes a profound neuroinflammatory response to remove the damaged tissue and prepare the brain for repair. However, the intense neuroinflammation occurring during the acute phase of stroke is associated with BBB breakdown, neuronal injury, and worse neurological outcomes. Here, we critically discuss the role of neuroinflammation in ischemic stroke pathology, focusing on the BBB and the interactions between central nervous system and peripheral immune responses. We highlight inflammation-driven injury mechanisms in stroke, including oxidative stress, increased MMP (matrix metalloproteinase) production, microglial activation, and infiltration of peripheral immune cells into the ischemic tissue. We provide an updated overview of imaging techniques for in vivo detection of BBB permeability, leukocyte infiltration, microglial activation, and upregulation of cell adhesion molecules following ischemic brain injury. We discuss the possibility of clinical implementation of imaging modalities to assess stroke-associated neuroinflammation with the potential to provide image-guided diagnosis and treatment. We summarize the results from several clinical studies evaluating the efficacy of anti-inflammatory interventions in stroke. Although convincing preclinical evidence suggests that neuroinflammation is a promising target for ischemic stroke, thus far, translating these results into the clinical setting has proved difficult. Due to the dual role of inflammation in the progression of ischemic damage, more research is needed to mechanistically understand when the neuroinflammatory response begins the transition from injury to repair. This could have important implications for ischemic stroke treatment by informing time- and context-specific therapeutic interventions.

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Section: Imaging Molecular Markers Of Inflammationmentioning

confidence: 99%

Neuroinflammation, Stroke, Blood-Brain Barrier Dysfunction, and Imaging Modalities

Candelario‐Jalil

Dijkhuizen

Magnus

2022

Stroke

268

150

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“…Superparamagnetic iron–platinum nanoparticles targeted with novel anti-Iba-1 together with MRI studied in a four-week post-stroke surviving rat brain were used to measure the spatio temporal changeson the microglial/macrophage. The data revealed that nanoparticle-enhanced MRI scans could be an effective monitoring method to understand the neuroinflammation development in live animals during the treatment of stroke as well as disease progression [ 153 ].…”

Section: Nanomaterials and Their Clinical Applicationmentioning

confidence: 99%

Broad-Spectrum Theranostics and Biomedical Application of Functionalized Nanomaterials

Alshamrani

2022

Polymers

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Nanotechnology is an important branch of science in therapies known as “nanomedicine” and is the junction of various fields such as material science, chemistry, biology, physics, and optics. Nanomaterials are in the range between 1 and 100 nm in size and provide a large surface area to volume ratio; thus, they can be used for various diseases, including cardiovascular diseases, cancer, bacterial infections, and diabetes. Nanoparticles play a crucial role in therapy as they can enhance the accumulation and release of pharmacological agents, improve targeted delivery and ultimately decrease the intensity of drug side effects. In this review, we discussthe types of nanomaterials that have various biomedical applications. Biomolecules that are often conjugated with nanoparticles are proteins, peptides, DNA, and lipids, which can enhance biocompatibility, stability, and solubility. In this review, we focus on bioconjugation and nanoparticles and also discuss different types of nanoparticles including micelles, liposomes, carbon nanotubes, nanospheres, dendrimers, quantum dots, and metallic nanoparticles and their crucial role in various diseases and clinical applications. Additionally, we review the use of nanomaterials for bio-imaging, drug delivery, biosensing tissue engineering, medical devices, and immunoassays. Understandingthe characteristics and properties of nanoparticles and their interactions with the biological system can help us to develop novel strategies for the treatment, prevention, and diagnosis of many diseases including cancer, pulmonary diseases, etc. In this present review, the importance of various kinds of nanoparticles and their biomedical applications are discussed in much detail.

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“…With the help of nanoparticles, MRI enables accurate monitoring of neuroinflammatory progression. Sillerud et al ( 2020 ) used novel anti-Iba-1-targeted superparamagnetic iron-platinum (FePt) nanoparticles in conjunction with T2-weighted magnetic resonance imaging (MRI) to measure the spatiotemporal changes of the microglial/macrophage activation in the living rat brain for 4 weeks post-stroke. They found that this approach could monitor the dynamic development of active neuroinflammation during stroke progression and treatment (Sillerud et al, 2020 ).…”

Section: Nanomaterials-based Diagnosismentioning

confidence: 99%

“…Sillerud et al ( 2020 ) used novel anti-Iba-1-targeted superparamagnetic iron-platinum (FePt) nanoparticles in conjunction with T2-weighted magnetic resonance imaging (MRI) to measure the spatiotemporal changes of the microglial/macrophage activation in the living rat brain for 4 weeks post-stroke. They found that this approach could monitor the dynamic development of active neuroinflammation during stroke progression and treatment (Sillerud et al, 2020 ). Jin et al ( 2016 ) show that magnetic resonance imaging (MRI) or Xenogen imaging combined with the labeling of SPIO-Molday ION Rhodamine-B (MIRB) can be used to monitor the dynamics of CD4(+) T cells in the ischemic area after stroke.…”

Section: Nanomaterials-based Diagnosismentioning

confidence: 99%

Recent Advances in Nanomaterials for Diagnosis, Treatments, and Neurorestoration in Ischemic Stroke

Lin

Tang

2022

Front. Cell. Neurosci.

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Stroke is a major public health issue, corresponding to the second cause of mortality and the first cause of severe disability. Ischemic stroke is the most common type of stroke, accounting for 87% of all strokes, where early detection and clinical intervention are well known to decrease its morbidity and mortality. However, the diagnosis of ischemic stroke has been limited to the late stages, and its therapeutic window is too narrow to provide rational and effective treatment. In addition, clinical thrombolytics suffer from a short half-life, inactivation, allergic reactions, and non-specific tissue targeting. Another problem is the limited ability of current neuroprotective agents to promote recovery of the ischemic brain tissue after stroke, which contributes to the progressive and irreversible nature of ischemic stroke and also the severity of the outcome. Fortunately, because of biomaterials’ inherent biochemical and biophysical properties, including biocompatibility, biodegradability, renewability, nontoxicity, long blood circulation time, and targeting ability. Utilization of them has been pursued as an innovative and promising strategy to tackle these challenges. In this review, special emphasis will be placed on the recent advances in the study of nanomaterials for the diagnosis and therapy of ischemic stroke. Meanwhile, nanomaterials provide much promise for neural tissue salvage and regeneration in brain ischemia, which is also highlighted.

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Longitudinal monitoring of microglial/macrophage activation in ischemic rat brain using Iba-1-specific nanoparticle-enhanced magnetic resonance imaging

Cited by 19 publications

References 55 publications

Neuroinflammation, Stroke, Blood-Brain Barrier Dysfunction, and Imaging Modalities

Neuroinflammation, Stroke, Blood-Brain Barrier Dysfunction, and Imaging Modalities

Broad-Spectrum Theranostics and Biomedical Application of Functionalized Nanomaterials

Recent Advances in Nanomaterials for Diagnosis, Treatments, and Neurorestoration in Ischemic Stroke

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