Longitudinal analysis of HIV-1 BF1 recombinant strains in vertically infected children from Argentina reveals a decrease in CRF12_BF pol gene mosaic patterns and high diversity of BF unique recombinant forms

Aulicino, Paula; Bello, Gonzalo; Guimarães, Monick Lindenmeyer; Ruchansky, Dora; Rocco, Carlos; Mangano, Andrea; Morgado, Mariza G.; Sen, Luisa

doi:10.1016/j.meegid.2010.11.008

Cited by 20 publications

(36 citation statements)

References 26 publications

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“…Mas, diferentemente de Estados Unidos e Europa, na década de 1990 surgiram as primeiras sequências discordantes. O subtipo F, inicialmente descrito no Gabão, passou a ser encontrado no Brasil (Louwagie et al, 1993;, e posteriormente na Argentina (Aulicino et al, 2011). Em pouco tempo formas recombinantes BF passaram a ser descritas (Sabino et al, 1994), e a primeira CRF BF, a CRF12_BF foi descrita em 2001 na Argentina .…”

Section: A Recombinação No Hiv-1unclassified

“…Na Argentina, a expansão dos recombinantes BF foi muito mais acentuada, dividida em duas epidemias, uma causada pelo subtipo B em HSHs, e outra causada pelos recombinantes BF em usuários de drogas injetáveis (UDIs) e heterossexuais . Desde 1996, os UDIs e heterossexuais passaram a constituir o maior grupo de risco , e o crescimento dos recombinantes foi um dos maiores registrados para o HIV-1 , gerando novas formas recombinantes e chegando a 85% dos casos de infecção infantil (Aulicino et al, 2011).…”

Section: A Recombinação No Hiv-1unclassified

“…Enquanto na Argentina ainda predominam variantes derivadas da CRF12_BF com linhagens definidas (Aulicino et al, 2011), o Brasil possui diversas CRFs com origens distintas (Thomson et al, 2004), e muita diversidade de URFs Tanuri et al, 1999;Vicente et al, 2000;Guimaraes et al, 2010). Ao contrário de CRFs mais bem estabelecidas, que causam grandes epidemias bem definidas, como as CRF01_AE e 16 1 INTRODUÇÃO Atila Iamarino CRF02_AG (Carr et al, 1996(Carr et al, , 1998Gao et al, 1996; Tebit e Arts, 2011), os recombinantes BF compõe diversas CRFs com múltiplos pontos de quebra.…”

Section: A Recombinação No Hiv-1unclassified

“…Retrospectivamente, o subtipo F e recombinantes derivados foram encontrados muito antes na Argentina, com evidências de 1985 (Aulicino et al, 2011). No Brasil, as primeiras sequências deste subtipo foram encontradas quase uma década depois (Louwagie et al, 1993(Louwagie et al, , 1994) -embora um dos pacientes recombinantes estudado aqui (0063) tenha sido diagnosticado em 1985 (Melo et al, 2011), não se pode afirmar com certeza que ele tenha sido infectado inicialmente por um vírus recombinante.…”

Section: Os Recombinantes Bfunclassified

“…Desta forma, recombinantes BF seguem como um dos maiores responsáveis por novos casos na América do Sul (Sucupira et al, 2007a;Teixeira et al, 2010;Aulicino et al, 2011), gerando novas URFs e CRFs, como a reavaliação de recombinantes BF realizada por Zhang et al indica, e dando …”

Section: Os Recombinantes Bfunclassified

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Análise e caracterização molecular, estrutural e populacional de proteases de HIV-1 do Estado de São Paulo.

Iamarino¹

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Section: A Recombinação No Hiv-1unclassified

Section: Os Recombinantes Bfunclassified

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Análise e caracterização molecular, estrutural e populacional de proteases de HIV-1 do Estado de São Paulo.

Iamarino¹

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Characterization of full-length HIV-1 CRF17_BF genomes and comparison to the prototype CRF12_BF strains

Aulicino

Gómez-Carrillo

Bello

et al. 2012

Infection, Genetics and Evolution

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The aim of this work is to characterize the full-length intersubtype recombinant structure of the HIV-1 Circulating Recombinant Form CRF17_BF. A single genome of CRF17_BF was originally described in 2001 as being largely similar to CRF12_BF. Since then, more genomes of CRF17_BF have been sequenced but not adequately described in publications. Here we describe CRF17_BF as a genuine CRF, and analyze its recombination pattern based on bootscan analyses, subtype signature patterns, and phylogenetic reconstruction of subtype-delimited segments. We show that CRF17_BF can be distinguished from CRF12_BF in several regions of the genome, including vpu, pol, env and nef. A complete and accurate characterization and description of recombination breakpoints in CRFs is required for a proper surveillance of HIV-1 genotypes, and important for epidemiological purposes.

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Relevance of Early Detection of HIV Type 1 SI/CXCR4-Using Viruses in Vertically Infected Children

Crudeli

Aulicino²,

Rocco³

et al. 2012

AIDS Research and Human Retroviruses

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The aim of the study was to investigate the prevalence and persistence of syncytium-inducing (SI) strains in HIV-1-infected children along time of infection and to evaluate the influence of antiretroviral therapy and host factors on viral tropism. This is a retrospective analysis carried out in 267 HIV-1 vertically infected children from an Argentinean cohort. The viral phenotype was screened in MT-2 cells and coreceptor usage confirmed by the GHOST cell assay. Also, CD4(+) T cell count, viral load, antiretroviral therapy, and human CCR5-Δ32 and CCR2-64I genotypes were analyzed. A high frequency of HIV-1 SI/CXCR4-using variants (22%) was found among children within the first trimester of life, reaching 46% after 10 years of infection. At acute infection, zidovudine prophylaxis did not significantly affect the proportions of SI HIV-1 strains, while their presence was favored by the CCR5(+)/Δ32 genotype. Interestingly, the majority of the early SI strains did not persist over time, probably due to a higher susceptibility to antiretroviral (ARV) treatment or immunologic pressure. At the chronic stage, SI variants emerged even in the presence of HAART reaching 36% at 120 months of infection. Also the HIV-1 SI phenotype was associated with lower CD4(+) T cell counts all along the course of infection. These findings highlight the need to evaluate the presence of SI/CXCR4 variants early at primary infection. This will make it possible to optimize the use of CCR5 inhibitors in children who are apparently carriers of the R5 virus preventing early therapeutic failure due to the reemergence of SI strains from reservoirs.

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Longitudinal analysis of HIV-1 BF1 recombinant strains in vertically infected children from Argentina reveals a decrease in CRF12_BF pol gene mosaic patterns and high diversity of BF unique recombinant forms

Cited by 20 publications

References 26 publications

Análise e caracterização molecular, estrutural e populacional de proteases de HIV-1 do Estado de São Paulo.

Análise e caracterização molecular, estrutural e populacional de proteases de HIV-1 do Estado de São Paulo.

Characterization of full-length HIV-1 CRF17_BF genomes and comparison to the prototype CRF12_BF strains

Relevance of Early Detection of HIV Type 1 SI/CXCR4-Using Viruses in Vertically Infected Children

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