Long-term storage of Narcissus anthers and pollen in liquid nitrogen

Bowes, S. A.

doi:10.1007/bf00023661

Cited by 17 publications

(12 citation statements)

References 15 publications

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“…An alternative technique is the storage of pollen at ultra-low temperatures in liquid nitrogen at -196 ° C. Pollen cryopreservation has been employed for a number of plants (Bowes, 1990;Towill, 1985), including Prunus species (Parfitt & Almehdi, 1984). In the latter study, in vivo germination of pollen recovered from cryogenic storage was demonstrated, although seed set was not reported.…”

Section: Introductionmentioning

confidence: 99%

Cryopreservation of pollen from two rose cultivars

et al. 1992

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An investigation was undertaken on the storage characteristics of pollen collected from two English rose cultivars. A rapid decline in viability was observed in pollen stored at + 4 ° C and-20 ° C, whereas the viability of pollen, stored at ultra-low temperature (-196 ° C), remained constant. Cryopreserved pollen was shown to retain its ability for fertilisation. The effects of the stage of flower development and anther dehiscence were assessed on both pre-and post-cryopreservation viabilities. Successful long-term storage of pollen will facilitate hybridisation of rose species and cultivars that do not flower synchronously.

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Section: Introductionmentioning

confidence: 99%

Cryopreservation of pollen from two rose cultivars

et al. 1992

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“…However, published reports on this aspect are restricted to a few ornamental crops. Among bulbous ornamentals, long-term pollen storage of Narcissus in liquid nitrogen is reported by Bowes (1990). In Gladiolus, pollen storage for duration of 730 days at À40 C has been reported by Koopwitz et al (1984) for five species.…”

Section: Cryopreservation Of Gladiolusmentioning

confidence: 90%

Gladiolus

Cantor

Janakiram

2011

Wild Crop Relatives: Genomic and Breeding Resources

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“…Published reports on cryogenic storage of pollen in other ornamental species show a reduction in seed recovery in Narcissus (Bowes, 1990) after 352 days of cryogenic storage. Marchant et al (1993) cryopreserved rose pollen for 8 weeks without any reduction in fertility levels.…”

Section: Discussionmentioning

confidence: 99%

“…Hughes & Lee (1991) successfully preserved Clianthus pollen at -1800 C. Marchant et al (1993) cryopreserved pollen of two English rose cultivars for 8 weeks. Among bulbous ornamentals, long-term pollen storage of Narcissus in liquid nitrogen is reported by Bowes (1990). In gladiolus, pollen storage for a duration of 730 days at -40 ° C has been reported by Koopowitz et al (1984) for 5 species.…”

Section: Introductionmentioning

confidence: 86%

Freeze preservation of gladiolus pollen

et al. 1994

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Viability and fertility profiles of cryopreserved gladiolus pollen from 7 cultivars have shown that it is possible to use cryogenic methods for conservation and management of the haploid gene pool in this species. There was no decline in pollen viability (in vitro) levels after 1 and 10 years of cryogenic storage. Field pollinations with cryogenic stored pollen induced capsule and seed set in varying capacities. Long-term cryogenic storage of gladiolus pollen could enhance breeding efficiency through better management of the haploid gene pool resources. Pollen parents could be made available throughout the breeding programme, ensuring guaranteed supply at the time of peak stigma receptivity. A 'pollen cryobank' facility established for this species would increase genetic diversity conservation at the haploid stage.

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Long-term storage of Narcissus anthers and pollen in liquid nitrogen

Cited by 17 publications

References 15 publications

Cryopreservation of pollen from two rose cultivars

Cryopreservation of pollen from two rose cultivars

Gladiolus

Freeze preservation of gladiolus pollen

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