Cryopreservation of pollen from two rose cultivars

Marchant, R.; Power, J. B.; Davey, M. R.; Chartier-Hollis, J. M.; Lynch, P. T.

doi:10.1007/bf00025309

Cited by 23 publications

(11 citation statements)

References 12 publications

(9 reference statements)

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“…The media were prepared with 0.015 g boric acid (Jicinska et al 1976;Pearson and Harney 1984;Gudin et al 1991a, b;Marchant et al 1993) dissolved in 200 mL water. Saccharose was added and the volume was adjusted to 300 mL.…”

Section: Saccharose Concentration In the Mediummentioning

confidence: 99%

“…The success or failure of germination depends on such factors as the period of the year during which the pollen is collected, the stage of development of the flower from which the pollen is collected, and the drying and storage techniques (Marchant et al 1993). Depending on the study, it is recommended that the pollen be collected when the sepals are open (Pearson and Harney 1984), when the outside petals begin to open (Marchant et al 1993) or when the flowers are fully open (Rajasekharan and Ganeshan 1994).…”

mentioning

confidence: 99%

“…The literature reports wide variations in the techniques used for drying the anthers and storing the pollen (Calvino 1951;Khan 1987;Marchant et al 1993;Visser et al 1977). Good pollen germination has been reported with drying at ambient temperature for 24 h (Jicinska et al 1976;Koncalova 1975) and 48 h , whereas Voyiatzi (1995) prefers a period of 1 h at 60% relative humidity.…”

mentioning

confidence: 99%

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Factors influencing pollen germination in three Explorer™ roses

Richer¹,

Poulin²,

Rioux

2007

Can. J. Plant Sci.

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Richer, C., Poulin, M. and Rioux, J.-A. 2007. Factors influencing pollen germination in three Explorer™ roses. Can. J. Plant Sci. 87: 115-119. Pollen germination is useful for the estimation of pollen viability. For three cultivars from the Explorer TM series, Champlain, Nicolas and Frontenac, a liquid medium with a pH of 5.6 and a saccharose concentration of 15% provides optimal germination conditions. Four flower stages were determined and evaluated according to the following scale: flower bud at stage 0 (closed bud), stage 1 (closed petals but open sepals), stage 2 (bud with petals three-quarters open) and stage 3 (fully open flower). Pollen germination was higher when the pollen was collected at stages 0 and 1 for Champlain, at stages 1 and 2 for Frontenac and at stage 2 for Nicolas. Based on the results of the evaluation of pollen drying techniques, it is recommended that pollen be dried for 24 h in darkness before use in crossing.

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Section: Saccharose Concentration In the Mediummentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Factors influencing pollen germination in three Explorer™ roses

Richer¹,

Poulin²,

Rioux

2007

Can. J. Plant Sci.

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“…So far cryopreservation procedures have been published for 21 species and varieties of roses. The first processes of germplasm cryopreservation were carried out on pollen (Marchant et al, 1993;Rajasekharan and Ganeshan, 1994). In recent years, buds…”

Section: Introductionmentioning

confidence: 99%

Integration of Cryopreservation and Tissue Culture for Germplasm Conservation and Propagation of Rosa pomifera ‘Karpatia’

Kwaśniewska

Dziedzic

Pawłowska

2017

Not Bot Horti Agrobo

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Cryopreservation is an useful technique for long-term conservation that requires minimal space and maintenance. Germplasm protection of Rosa is important to preserve genetic diversity, to store material for breeding and to expand new research. This study was conducted to develop a droplet vitrification cryopreservation and micropropagation of Rosa pomifera cv. 'Karpatia', whose large hypanthia are characterized by remarkable pro-health properties. Cultures in vitro were stabilized and shoot tips collected from dormant buds served as initial explants. The multiplication of shoots was carried out on MS medium containing benzyladenine. For the droplet vitrification cryopreservation, shoot tips from in vitro cultures were used: small with exposed meristem and large with a meristem covered with leaves, as well as shoot tips from in situ plants, which were collected in winter. Treatment time with plant vitrification solution (PVS2) was also tested (10-30 minutes). From in vitro culture, 32-41% small explants with exposed meristem survived, but they regenerated at a very low level. The best cryostorage results were obtained for shoot tips from dormant buds and a 20-minute PVS2 treatment: the survival was 84% and regeneration 73%. During the post-freezing regeneration multiplication index was 2.4 shoots per one multiplication cycle, after cryopreservation and in the control. On half MS medium without growth regulators, 97-99% of shoots rooted, and all rooted plants have adapted to ex vitro conditions and were planted into the soil. Biometric analyses during shoot multiplication, rooting and acclimatization stages did not reveal any changes compared to the non-cryopreserved samples.

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“…However, published reports on this aspect are restricted to a few ornamental crops. Hughes & Lee (1991) successfully preserved Clianthus pollen at -1800 C. Marchant et al (1993) cryopreserved pollen of two English rose cultivars for 8 weeks. Among bulbous ornamentals, long-term pollen storage of Narcissus in liquid nitrogen is reported by Bowes (1990).…”

Section: Introductionmentioning

confidence: 99%

Freeze preservation of gladiolus pollen

et al. 1994

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Viability and fertility profiles of cryopreserved gladiolus pollen from 7 cultivars have shown that it is possible to use cryogenic methods for conservation and management of the haploid gene pool in this species. There was no decline in pollen viability (in vitro) levels after 1 and 10 years of cryogenic storage. Field pollinations with cryogenic stored pollen induced capsule and seed set in varying capacities. Long-term cryogenic storage of gladiolus pollen could enhance breeding efficiency through better management of the haploid gene pool resources. Pollen parents could be made available throughout the breeding programme, ensuring guaranteed supply at the time of peak stigma receptivity. A 'pollen cryobank' facility established for this species would increase genetic diversity conservation at the haploid stage.

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Cryopreservation of pollen from two rose cultivars

Cited by 23 publications

References 12 publications

Factors influencing pollen germination in three Explorer™ roses

Factors influencing pollen germination in three Explorer™ roses

Integration of Cryopreservation and Tissue Culture for Germplasm Conservation and Propagation of Rosa pomifera ‘Karpatia’

Freeze preservation of gladiolus pollen

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