Freeze preservation of gladiolus pollen

Rajasekharan, P. E.; Rao, T. M.; Janakiram, T.; Ganeshan, S.

doi:10.1007/bf00039304

Cited by 28 publications

(14 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In tests carried out in vitro after 3 months of storage, germination did not exceed 11%, with no significant effect of temperature (data not shown). However, pollen that does not germinate well on media suitable for freshly collected pollen is not necessarily nonviable Rajasekharan et al, 1994;Shivanna and Rangaswamy, 1992) and may produce satisfactory fruit set. Moreover, if the pollen load on the stigma is adequate, a sufficient number of pollen grains will be able to ensure fertilization.…”

Section: Resultsmentioning

confidence: 99%

Viability of Pollen of Two Fruit Crop Cacti of the Genus Hylocereus Is Affected by Temperature and Duration of Storage

Metz¹,

Nerd²,

Mizrahi³

2000

HortSci

View full text Add to dashboard Cite

Hylocereus undatus (Haw.) and H. polyrhizus (Weber) are new fruit crops of the Cactaceae. In Israel, flowers of the two species, which are self-incompatible, are hand cross-pollinated. In order to ensure a current supply of compatible pollen and guarantee good yields, we have developed a procedure for long-term storage of pollen. Pollen for storage was collected in the evening or in the morning. Its moisture content ranged between 45% to 50% in the evening and between 18% to 22% in the morning. Pollen was first dehydrated in a vacuum desiccator until the moisture content was reduced to 5% to 10% and then stored at various temperatures (+4, -18, -70, -196 °C) for 3 or 9 months, after which it was used for cross-pollination. Percent fruit set and fruit fresh weight (FW) were affected by the temperature but not the duration of pollen storage; storage at +4 °C reduced fruit set, fruit FW, and seed number more than did storage at subfreezing temperatures. The FW of fruits produced by frozen pollen was similar to that produced by fresh pollen in commercial orchards. The rate of seed germination was high (≈90%) regardless of the temperature during pollen storage.

show abstract

Section: Resultsmentioning

confidence: 99%

Viability of Pollen of Two Fruit Crop Cacti of the Genus Hylocereus Is Affected by Temperature and Duration of Storage

Metz¹,

Nerd²,

Mizrahi³

2000

HortSci

View full text Add to dashboard Cite

show abstract

“…The interaction effect of cryopreserved pollen-cum-Psittacinus hybrid x Kum Kum produced minimum number of underdeveloped seeds (8.00 per capsule) which was on par with cryoprerved pollen-cum-Hybrid selection 88-10-22 x Shobha (8.88 per capsule), and fresh pollen-cumPsittacinus hybrid x Kum Kum (9.56 per capsule). Rajasekharan et al (1994) observed comparatively high number of seeds per capsule under fresh pollen used in cross combinations viz. Sapna x Meera, Shobha x Meera and Meera x Aspara.…”

Section: Interaction Effect Of Pollen (Fresh or Cryopreserved) And Crmentioning

confidence: 92%

Effect of Fresh or Cryopreserved Pollen on Seed Setting of Intervarietal and Interspecific Crosses of Gladiolus

Poon

Rao

Rajshekharan

et al. 2013

Nepal Journal of Science and Technology

View full text Add to dashboard Cite

An experiment was conducted in the field of Indian Institute of Hortricultural Research, Hessaraghatta, Bangalore during August 2006 to June 2008. The experiment consisting of eight treatment combinations were laid out in '2x4' factorial RCBD with 5 replications to assess seed set per capsule. The results revealed that highly significant differences were observed for number of well-developed seeds and underdeveloped seeds. Among four cross combinations 'H.S. 88-10-22 x Shobha' (27.63 well-developed seeds per capsule) and 'H.S. 84-7-11 x Shobha' (27.01 well-developed seeds per capsule) were significantly higher than those of other two cross combinations. The interaction effect of fresh pollen-cum-'H.S.88-10-22 x Shobha' produced significantly higher number of welldeveloped seeds (41.02 per capsule) than those of the other treatment combinations. In contrast, treatment combinations viz., cryopreserved pollen-cum-'H.S. 88-10-22 x Shobha' produced the lowest number of welldeveloped seeds (10.85 per capsule).Inclusion of fresh pollen to genotype 'Shobha' and cryopreserved pollen of H.S. 88-10-22' revealed better performances for maximum seed set per capsule.

show abstract

“…In tests carried out in vitro after 3 months of storage germination did not exceed 11%, with no significant effect of temperature (data not shown). However, pollen that does not germinate well on media suitable for freshly collected pollen is not necessarily nonviable (Polito and Luza, 1988;Rajasekharan et al, 1994;Shivanna and Rangaswamy, 1992) and may produce satisfactory fruit set. Moreover, if the pollen load on the stigma is adequate, a sufficient number of pollen grains will be able to ensure fertilization.…”

Section: Resultsmentioning

confidence: 99%

Viability of Pollen of Two Fruit Crop Cacti of the Genus Hylocereus Is Affected by Temperature and Duration of Storage

Metz¹,

Nerd²,

Mizrahi³

2000

HortSci

View full text Add to dashboard Cite

Hylocereus undatus (Haw.) and H. polyrhizus (Weber) are new fruit crops of the Cactaceae. In Israel, flowers of the two species, which are self-incompatible, are hand cross-pollinated. In order to ensure a current supply of compatible pollen and guarantee good yields, we have developed a procedure for long-term storage of pollen. Pollen for storage was collected in the evening or in the morning. Its moisture content ranged between 45% to 50% in the evening and between 18% to 22% in the morning. Pollen was first dehydrated in a vacuum desiccator until the moisture content was reduced to 5% to 10% and then stored at various temperatures (+4, –18, –70, –196 °C) for 3 or 9 months, after which it was used for cross-pollination. Percent fruit set and fruit fresh weight (FW) were affected by the temperature but not the duration of pollen storage; storage at +4 °C reduced fruit set, fruit FW, and seed number more than did storage at subfreezing temperatures. The FW of fruits produced by frozen pollen was similar to that produced by fresh pollen in commercial orchards. The rate of seed germination was high (≈90%) regardless of the temperature during pollen storage.

show abstract

Freeze preservation of gladiolus pollen

Cited by 28 publications

References 9 publications

Viability of Pollen of Two Fruit Crop Cacti of the Genus Hylocereus Is Affected by Temperature and Duration of Storage

Viability of Pollen of Two Fruit Crop Cacti of the Genus Hylocereus Is Affected by Temperature and Duration of Storage

Effect of Fresh or Cryopreserved Pollen on Seed Setting of Intervarietal and Interspecific Crosses of Gladiolus

Viability of Pollen of Two Fruit Crop Cacti of the Genus Hylocereus Is Affected by Temperature and Duration of Storage

Contact Info

Product

Resources

About