Effect of Fresh or Cryopreserved Pollen on Seed Setting of Intervarietal and Interspecific Crosses of Gladiolus

Abstract

An experiment was conducted in the field of Indian Institute of Hortricultural Research, Hessaraghatta, Bangalore during August 2006 to June 2008. The experiment consisting of eight treatment combinations were laid out in '2x4' factorial RCBD with 5 replications to assess seed set per capsule. The results revealed that highly significant differences were observed for number of well-developed seeds and underdeveloped seeds. Among four cross combinations 'H.S. 88-10-22 x Shobha' (27.63 well-developed seeds per capsule) and 'H.S. 84-7-11 x Shobha' (27.01 well-developed seeds per capsule) were significantly higher than those of other two cross combinations. The interaction effect of fresh pollen-cum-'H.S.88-10-22 x Shobha' produced significantly higher number of welldeveloped seeds (41.02 per capsule) than those of the other treatment combinations. In contrast, treatment combinations viz., cryopreserved pollen-cum-'H.S. 88-10-22 x Shobha' produced the lowest number of welldeveloped seeds (10.85 per capsule).Inclusion of fresh pollen to genotype 'Shobha' and cryopreserved pollen of H.S. 88-10-22' revealed better performances for maximum seed set per capsule.

Characterization of Gladiolus Genotypes under Khumaltar Condition of Nepal

“…The variation in number of florets opened per spike might be attributed to the differential numbers of florets per spike and accumulation of carbohydrates due to varied leaf production in turn, variation in these aspects might be due to genetic make-up of the plants (Shakya 2006). Varietal variation in number of florets per spike was also reported by (Sindhu and Verma 1995), (Regmi 2000) and (Poon 2009). The plant height, spike length and rachis length also varied enormously among the genotypes which might be due to differences in size of corms i.e., largest corms produced tallest plant with high spike and rachis length while smallest sized corms produced the plants with the shortest height with short spike and rachis length.…”

Influence of Intervarietal and Interspecific Crosses on Seed Set of Gladiolus under Mid- hill Environments of Dailekh Condition

“…Moreover, relatively high number of developed seeds (55.9 per capsule) was recorded from the crosses between 'Psittacinus hybrid and G-4 in the similar study under Dailekh condition, Nepal when crosses were made from the first fortnight of April to the first fortnight of May. The findings of the two studies formerly made by Dhaduk et al (1987) and Poon (2009) are quite different from that of the recent study most presumably due to the inclusion of different genotypes as male parents in crosses, some of flower abortions at low temperature and low light intensity during early January and mid February (12.0 0 -29.0 0 C) under Bengluru environments, India. Moderate high temperature (15 0 -32 0 C) during April and May possibly could produce a positive effect in overcoming incongruity in interspecific crosses of gladiolus under mid-hill environments of Dailekh, Nepal.…”

“…Dhaduk et al (1987) reported that varieties viz., 'Sylvia','Psittacinus hybrid', Jogenaar' did not set capsule at all under natural self pollination, and 'Psittacinus hybrid x Sancerre' produced the low number of seed per capsule (0.38) under artificial cross pollination. Poon (2009) recorded the high number of developed seeds (15.22 per capsule) from the crosses between 'Psittacinus hybrid and Kum Kum' under Bengaluru condition, India when crosses were made from early January to mid February. Moreover, relatively high number of developed seeds (55.9 per capsule) was recorded from the crosses between 'Psittacinus hybrid and G-4 in the similar study under Dailekh condition, Nepal when crosses were made from the first fortnight of April to the first fortnight of May.…”

“…• Matured capsule must be harvested in approximately a month after the day of crosspollination depending upon the inclusion of varieties or species in crosses. • Getting success in viable seed set is almost always difficult when interspecific crosses are made from G. grandiflorus x G. calianthus; however, even under the open field condition, viable seed sets resulted in the interspecific crosses made from G. grandiflorus x G. psittacinus (Poon 2009, Personal experience 2011). Hence, inclusion of G. psittacinus as one of the parent will render the interspecific hybridization program deserving in future.…”