Long-Term Quality Control of the Cytokine &amp; Growth Factors and Cell Adhesion Molecule Arrays at the Randox Evidence Investigator

Pelt, Johannes van; Romijn, Fred P.H.T.M.

doi:10.2478/v10011-009-0031-x

Cited by 5 publications

(4 citation statements)

References 8 publications

(1 reference statement)

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“…To infiltrate sites of inflammation, eosino phils leave the bloodstream and pass through the endothelium in four steps, namely rolling, adhesion, transendothelial mi gration, and chemotaxis (24). Ad hesion molecules, such as VCAM-1, play an important role during adhesion to endothelial cells (17,24,25). Experiments performed by Ohori et al (24) demon strated that TNF-a sti mulation induces VCAM-1 protein and mRNA expression in human nasal polyp fibroblasts.…”

Section: Discussionmentioning

confidence: 99%

Cytokine Profiles in Nasal Fluid in Patients with Nasal Polyps: A Flow Cytometric Study

Perić¹,

Vojvodić²,

Radulović³

2010

Journal of Medical Biochemistry

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Cytokine Profiles in Nasal Fluid in Patients with Nasal Polyps: A Flow Cytometric StudyBiological markers in nasal fluid provide valuable information on nasal pathophysiology. The aims of this study were to compare the cytokine profiles of nasal fluid in subjects with nasal polyps (NP) and co-morbid asthma and NP patients without asthma and to determine the role of these cytokines in the development of NP. Thirty patients with NP (15 asthmatic and 15 non asthmatic) were included in this prospective study. Nasal secretion samples were collected from nasal cavities of all 30 subjects. The levels of eleven cytokines (TNF-α, TNF-β, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12, and IFN-γ) were measured using flow cytometry. The concentrations of Th2 cytokines IL-5, IL-6 and IL-10 were significantly higher in patients with NP and asthma compared with subjects with NP without asthma. We also found significantly higher levels of IFN-α, IL-4, IL-6 and IL-10 in allergic patients with NP and asthma compared with those without asthma. In nonallergic patients with NP and asthma, the concentrations of TNF-α, IL-5 and IL-6 were significantly higher than in nonallergic patients with NP without asthma. Our results show that the presence of Th2 cytokines, especially IL-5 and IL-6 in patients with NP and asthma is a more prominent feature than in those without asthma that relates to the increased eosinophilic inflammation. We have also found a significant influence of allergy on the cytokine profiles both in asthmatic and nonasthmatic patients.

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Section: Discussionmentioning

confidence: 99%

Cytokine Profiles in Nasal Fluid in Patients with Nasal Polyps: A Flow Cytometric Study

Perić¹,

Vojvodić²,

Radulović³

2010

Journal of Medical Biochemistry

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“…Adhesion molecules, ICAM-1 and VCAM-1, are essential for cell-cell interactions, being also important in cell activation and adhesion (51). Soluble adhesion molecules may be detected in patient sera as useful markers of leucocytes and endothelial cells activation in different human diseases, including SLE and LV.…”

Section: Discussionmentioning

confidence: 99%

Serum Levels and in Vitro Production of Th1- and Th2-Type Cytokines by Peripheral Blood Mononuclear Cells in Patients Suffering from Systemic Lupus Erythematosus

Đorđević¹,

Zvezdanovic²,

Ćosić³

et al. 2010

Journal of Medical Biochemistry

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Kratak sadr`aj: U serumu bolesnika sa sistemskim lupusom eritematodesom odre|ivani su Th1 i Th2 citokini i adhezioni molekuli a u kulturi mononuklearnih }elija periferne krvi (PBMC) produkcija citokina. Faktor tumorske nekroze-alfa (TNF-a), interferon-gama (IFN-g), interleukin1b (IL-1b), IL-4, IL-10, IL-13, inter}elijski adhezioni molekul-1 (ICAM-1) i vaskularni }elijski adhezioni molekul-1 (VCAM-1) mereni su ELISA tehnikom u serumu 16 bolesnika sa sistemskim lupusom eritematodesom bez vaskulita (SLE), 30 bolesnika sa SLE koji su imali vaskulitis i 18 zdravih kontrola. Citokini su, tako|e, odre|ivani u kulturi nestimulisanih kao i konkanavalinom-A (Con-A) i forbol-12--miristat-13-acetatom (PMA) stimulisanih PBMC. Serumski nivoi TNF-a su zna~ajno povi{eni kod obe grupe bolesnika, dok je IL-1b povi{en samo u grupi sa SLE. Serumski TNF-a je, tako|e, zna~ajno vi{i kod bolesnika sa SLE u pore |enju sa LV grupom. Sva tri Th2 citokina su zna~ajno vi{a kod obe grupe bolesnika u pore|enju sa zdravim ispitanicima. Nivoi IFN-g i Th2 citokina zna~ajno su vi{i kod bolesnika sa te`im oblikom bolesti. Kod bolesnika sa SLE zna~ajno su povi {e ne koncentracije oba adheziona molekula, dok je kod bolesnika sa LV povi{en samo VCAM-1. Kod obe grupe bolesnika postoji zna~ajna korelacija ICAM-1 i IL-1b, IFN-g, IL-4 i IL-10. U grupi sa SLE VCAM-1 zna~ajno koreSummary: Th1-type and Th2-type cytokine profiles and adhesion molecules in the serum of patients suffering from systemic lupus erythematosus and the cytokine production by peripheral blood mononuclear cells (PBMC) were studied. Tumor necrosis factor-alpha (TNF-a), interferongamma (IFN-g), interleukin-1b (IL-1b), IL-4, IL-10, IL-13, intercellular adhe sion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) were measured using ELISA technique in the sera of 16 systemic lupus erythematosus patients without vasculitis (SLE), 30 SLE patients with vasculitis (LV), and in 18 healthy controls. The cytokines were also measured in the culture media of unstimulated, concana valin-A (Con-A) and phorbol-12-myristate-13-acetate (PMA) stimulated PBMC. TNF-a serum levels were significantly elevated in both SLE and LV patients and those of IL-1b in SLE patients. TNF-a was also signi ficantly incre ased in SLE compared to LV patients. Serum levels of all three Th-2 cytokines were significantly ele vated in both SLE and LV patients compared to healthy controls. Serum IFN-g and Th2 cytokine levels were significantly increased in patients with more active disease. Both ICAM-1 and VCAM-1 were significantly increased in SLE patients and only VCAM-1 in LV patients. ICAM-1 showed a significant correlation with IL-1b, IFN-g, IL-4 and IL-10 in both

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“…This chemiluminescent multiplexed immunoassay Glass slide-based system A technology developed by Randox Laboratories Ltd [25,26] was published by Sachdeva and coworkers in 2007. The system was a sandwich Reproduced with permission from [23].…”

Section: Multiplexed Chemiluminescent Immunoassaysmentioning

confidence: 99%

Recent Advances in Multiplex Immunoassays

2012

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The present review reports on the lastest developments in multiplex immunoassays. The selected examples are classified through their detection strategy (fluorescence, chemiluminescence, colorimetry or labeless) and their assay format (standard microtiter plate, polymeric membranes and glass slides). Finally, the degree of integration in a complete system, incorporating fluid handling and detection was also taken into account.

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Long-Term Quality Control of the Cytokine & Growth Factors and Cell Adhesion Molecule Arrays at the Randox Evidence Investigator

Cited by 5 publications

References 8 publications

Cytokine Profiles in Nasal Fluid in Patients with Nasal Polyps: A Flow Cytometric Study

Cytokine Profiles in Nasal Fluid in Patients with Nasal Polyps: A Flow Cytometric Study

Serum Levels and in Vitro Production of Th1- and Th2-Type Cytokines by Peripheral Blood Mononuclear Cells in Patients Suffering from Systemic Lupus Erythematosus

Recent Advances in Multiplex Immunoassays

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