Cytokine Profiles in Nasal Fluid in Patients with Nasal Polyps: A Flow Cytometric Study

Perić, Aleksandar; Vojvodić, Danilo; Radulović, Vesna

doi:10.2478/v10011-010-0003-1

Cited by 7 publications

(12 citation statements)

References 20 publications

(32 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…IL-17 in the sputum, bronchoalveolar lavage fluid and serum has been found to markedly increase in asthma patients, and the extent of increase of the IL-17 level is related to the airway hyperrespon siveness and the degree of inflammation (13). Klemens et al (14) reported that IL-17 was detectable in the excretions of viral rhinitis patients and AR patients, but the IL-17 level in the viral rhinitis was dramatically higher than that in the AR patients. In addition, Ciprandi et al (15) found, in the pollen season, the serum level of IL-17 was closely relevant with the severity of clinical manifestations in patients with pollen induced AR.…”

Section: Discussionmentioning

confidence: 99%

Expressions of IL-17, IL-21 and IL-23 in the Serum of Allergic Rhinitis Patients

Huang¹,

Yang²,

Chen³

et al. 2011

Journal of Medical Biochemistry

View full text Add to dashboard Cite

Expressions of IL-17, IL-21 and IL-23 in the Serum of Allergic Rhinitis PatientsThe present study aimed to investigate the expressions of interleukin-17 (IL-17), IL-21 and IL-23 in the serum of allergic rhinitis (AR) patients and to explore their relationship with special IgE (sFgE) in the serum. AR patients (n=24) and healthy subjects (n=12) were recruited and serum samples were collected. The serum level of IgE specific for inhalant allergens was determined using the automatic quantitative immunofluorescence analysis system, and the contents of IL-17, IL-21 and IL-23 in the serum were detected using ELISA. The level of serum IgE in the healthy individuals was categorized as grade 0 and that in the AR patients as grade 2-6. The mean contents of IL-17, IL-21 and IL-23 were 164.71 ±39.37 pg/mL, 199±97.86 pg/mL and 78.94±26.33 pg/mL, respectively, in the AR patients, and 67.75±18.24 pg/mL, 7.58±5.49 pg/mL and 13.58± 3.93 pg/mL, respectively, in the healthy subjects. Statistical analysis showed the serum levels of IL-17 and IL-23 in the AR patients were markedly higher than those in the healthy subjects, however, no significant difference was noted in the content of IL-21. Furthermore, the IL-17 level was positively related to the levels of IL-23 and IgE and the IL-23 level was positively related to the IgE level among AR patients, but no relations were observed between the IL-21 level and levels of IL-17, IL-23 and IgE. Our study indicates IL-17 and IL-23 may play an important role in the pathogenesis of AR and maybe IL-21 is not involved in the occurrence of AR.

show abstract

Section: Discussionmentioning

confidence: 99%

Expressions of IL-17, IL-21 and IL-23 in the Serum of Allergic Rhinitis Patients

Huang¹,

Yang²,

Chen³

et al. 2011

Journal of Medical Biochemistry

View full text Add to dashboard Cite

show abstract

“…Drveni štapić sa vatom na vrhu (deo sa vatom je dužine oko 10 mm, a širine oko 4 mm), tokom prednje rinoskopije postavlja se što bliže ili se drži u srednjem nosnom hodniku tokom 60 sekundi, koliko je potrebno da se komadić vate natopi nosnim sekretom (slika 2). Zatim se deo štapića sa vatom na vrhu odlomi i ubaci u ependorf epruvetu u kojoj se nalazi transfer-medijum (18)(19)(20)(21)(22)(23).…”

Section: ) Apsorpcione Tehnikeunclassified

“…Pošto se uzme uzorak nosnog sekreta, on se unosi u ependorf epruvetu u kojoj se nalazi 1 ml "transfer medijuma" (18)(19)(20)(21)(22)(23). To je fosfatno puferisani fiziološki rastvor sa 50 μg/ml gentamicina, 340 μg/ml penicilina G i 500 μg/ml fungizona, koji služe kao konzervansi i sprečavaju razmnožavanja bakterijskih i gljivičnih mikroorganizama u uzorku (18)(19)(20)(21)(22)(23). U "transfer medijumu" uzorak stoji minimalno 30 minuta, koliko je potrebno za difuziju citokina/hemokina iz uzorka sekreta, koji je hiperosmolalan u "transfer medijum", koji je hipoosmolalan.…”

Section: Merenje Protočnom Citometrijomunclassified

Sampling of nasal secretions for flow-cytometric analysis in inflammatory diseases of the nose and paranasal sinuses

Perić¹,

Vojvodić²,

Milojević³

et al. 2013

Medicinski casopis

View full text Add to dashboard Cite

Nasal secretions represent the first line of defense medium, in which the leucocytes probably act as an efficient part of the defense mechanism along with the mucociliary transport system and the biochemical properties of the mucus. The aim of this review article is to introduce all techniques for nasal secretion sampling used up to now. We described aspiration, absorption, and dilution techniques for nasal fluid collection. We, also, noted all limitations of these techniques. We especially described absorption technique with its advantages and limitations. Furthermore, we described a technique for nasal fluid samples preparation and for inflammatory mediator measurement -flow cytometry bead-based multiple analyte detection. Nasal secretion content reflects the inflammatory status of the nasal/paranasal sinuses mucosa and the evolution of mucosal disease. Med Čas (Krag) / Med J (Krag) 2013; 47 (1): 30-35. Slika 2. Modifikovana apsorpciona tehnika za uzorkovanje nosnog sekreta: štapić sa vatom na vrhu je uveden u srednji nosni hodnik.

show abstract

“…Nasal fluid samples were collected from nasal cavities of all 40 subjects (22 patients with nasal polyposis, and 18 patients with nasal polyposis and allergic rhinitis) before and within the seven days after the CAM treatment using modified absorption technique by placing cottonwool sticks (length 10 millimetres, diameter 4 millimetres; Institute of Virology, Vaccines and Sera, "Torlak", Belgrade, Serbia), into the nasal cavity posterior to the mucocutaneous junction for 60 seconds, as previously described 9,10 . All samples were placed in a 2 ml Eppendorf tube containing 1 ml of transfer medium (phosphatebuffered saline with gentamycin 50 μg/ml, penicillin G 340 U/ml, fungizone 500 μg/ml) for 30 min to allow diffusion of cytokines into the medium and then stored at 4 °C for a maximum of 2 h until processed.…”

Section: Sampling Of Nasal Fluid and Cytokine Determinationmentioning

confidence: 99%

Influence of Allergy on the Immunomodulatory and Clinical Effects of Long-Term Low-Dose Macrolide Treatment of Nasal Polyposis

Perić¹,

Vojvodić²,

Baletić³

et al. 2010

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub

View full text Add to dashboard Cite

Aims. Cytokine levels in nasal secretions reflect the inflammatory status of the nasal and paranasal sinus mucosa and the development of mucosal disease. The results of previous investigations suggest that macrolide antibiotics can be effective in treatment of chronic rhinosinusitis and nasal polyposis. The aim of this prospective study was to compare the immunomodulatory and clinical effects of long-term low-dose macrolide treatment of nonatopic and atopic patients with nasal polyposis.Methods. Forty (n = 40) patients with nasal polyposis, 22 allergic and 18 nonallergic were administered clarithromycin (CAM) 500 mg/day single oral dose for eight weeks. We measured the levels of proinflammatory Th1 cytokines TNF-α and IL-1β, Th2 cytokines IL-4, IL-5 and IL-6, and chemokine IL-8 in the nasal fluid samples, before and after treatment, using flow cytometric method. We also scored each of the 40 patients before and after therapy according to nasal symptom score and endoscopic score.Results. Following treatment, we found significantly reduced levels of IL-8 (p<0.01) and TNF-α (p<0.01) in nasal secretions in nonallergic patients. In subjects with nasal polyposis and allergy, we found decreased levels of IL-8 (p<0.01), IL-6 (p<0.05) and IL-1β (p<0.01). Macrolide therapy decreased the size of polyps in 45.45% of nonatopic and in 50% of atopic patients. After macrolide treatment, we found 67.83% patients in nonallergic group and 55.55% patients in allergic group with improved nasal symptoms.Conclusions. Long-term low-dose treatment with CAM was effective in the management of nasal polyposis. Our results showed that macrolide treatment of nasal polyposis have different immunomodulatory and similar clinical effects in allergic and nonallergic patients.

show abstract

Cytokine Profiles in Nasal Fluid in Patients with Nasal Polyps: A Flow Cytometric Study

Cited by 7 publications

References 20 publications

Expressions of IL-17, IL-21 and IL-23 in the Serum of Allergic Rhinitis Patients

Expressions of IL-17, IL-21 and IL-23 in the Serum of Allergic Rhinitis Patients

Sampling of nasal secretions for flow-cytometric analysis in inflammatory diseases of the nose and paranasal sinuses

Influence of Allergy on the Immunomodulatory and Clinical Effects of Long-Term Low-Dose Macrolide Treatment of Nasal Polyposis

Contact Info

Product

Resources

About