Long-Term Production of Ergot Peptides by Immobilized Claviceps purpurea in Semicontinuous and Continuous Culture

Abstract

The semicontinuous and continuous production of pharmaceutically useful ergot peptides with immobilized Clavicepspurpurea could be demonstrated. A key aspect was the presence of high concentrations of CaCl2 (96.9 mM) to give marked prolongation of the productive phase, and cultivation in a bubble column reactor became possible. Restriction of the phosphate supply avoided an otherwise problematic massive increase of outgrowing hyphae.

Continuous production of bacteriocins, brevicin, nisin and pediocin, using calcium alginate‐immobilized bacteria

“…for a-amylase production, the beads were reported to be stable and showed 36% effective production for up to 400 h (Jamuna and Ramakrishna 1992). Dierkes et al (1993) reported that the stability of alginate beads in growth media was improved by limiting the phosphate concentration and adding CaClz to the media. In this study, removal of phosphate, acetate and citrate and the addition of 10 mmol I-' CaClz to the elution media also substantially improved the physical stability of alginate beads.…”