Special Morphological and Metabolic Behavior of Immobilized Microorganisms

Rehm, Hans‐Jürgen; Omar, Sanaa H.

doi:10.1002/9783527620999.ch6

Biotechnology Set 2001

DOI: 10.1002/9783527620999.ch6

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Special Morphological and Metabolic Behavior of Immobilized Microorganisms

Hans‐Jürgen Rehm¹,

Sanaa H. Omar²

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Cited by 3 publications

(1 citation statement)

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“…Altered metabolism in immobilized cells has been found in many different systems (De Bont et al 1990;Rehm and Omar 1993), even though the mechanisms underlying immobilized cell response are still open to speculation. Changes in cellular activity have been attributed to diffusional limitations affecting the physical and chemical environment of immobilized cells and (or) to actual modifications of cellular physiology induced by immobilization (Norton and D'Amore 1994).…”

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confidence: 99%

Clues to the origin of high external invertase activity in immobilized growing yeast: prolongedSUC2transcription and less susceptibility of the enzyme to endogenous proteolysis

Alteriis

Alepuz²,

Estruch³

et al. 1999

Can. J. Microbiol.

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Expression of the SUC2 gene encoding invertase was studied using free and gelatin-immobilized yeast cells to try to explain the high activity of this enzyme exhibited by immobilized cells when allowed to grow in a nutrient medium. The results indicated that at least two factors are probably responsible for the accumulation of invertase in immobilized cells. First, the expression of the SUC2 gene was maintained throughout growth in immobilized cells, whereas its expression was only transient in free cells. Second, invertase of immobilized cells was shown to be less susceptible to endogenous proteolytic attack than that of the corresponding free cells. These results have been interpreted, respectively, in terms of diffusional limitations and changes in the pattern of invertase glycosylation due to growth of yeast in an immobilized state.

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mentioning

confidence: 99%

Clues to the origin of high external invertase activity in immobilized growing yeast: prolongedSUC2transcription and less susceptibility of the enzyme to endogenous proteolysis

Alteriis

Alepuz²,

Estruch³

et al. 1999

Can. J. Microbiol.

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Staphylococcus carnosus: from starter culture to protein engineering platform

Löfblom

Rosenstein

Nguyen

et al. 2017

Appl Microbiol Biotechnol

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Since the 1950s, Staphylococcus carnosus is used as a starter culture for sausage fermentation where it contributes to food safety, flavor, and a controlled fermentation process. The long experience with S. carnosus has shown that it is a harmless and “food grade” species. This was confirmed by the genome sequence of S. carnosus TM300 that lacks genes involved in pathogenicity. Since the development of a cloning system in TM300, numerous genes have been cloned, expressed, and characterized and in particular, virulence genes that could be functionally validated in this non-pathogenic strain. A secretion system was developed for production and secretion of industrially important proteins and later modified to also enable display of heterologous proteins on the surface. The display system has been employed for various purposes, such as development of live bacterial delivery vehicles as well as microbial biocatalysts or bioadsorbents for potential environmental or biosensor applications. Recently, this surface display system has been utilized for display of peptide and protein libraries for profiling of protease substrates and for generation of various affinity proteins, e.g., Affibody molecules and scFv antibodies. In addition, by display of fragmented antigen-encoding genes, the surface expression system has been successfully used for epitope mapping of antibodies. Reviews on specific applications of S. carnosus have been published earlier, but here we provide a more extensive overview, covering a broad range of areas from food fermentation to sophisticated methods for protein-based drug discovery, which are all based on S. carnosus.

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Influence of Different Salinity Levels and Immobilization on Glycerol Production by Halotolerant Yeast Zygosaccharomyces rouxii

Abo-State¹

2001

J. of Biological Sciences

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Special Morphological and Metabolic Behavior of Immobilized Microorganisms

Cited by 3 publications

References 70 publications

Clues to the origin of high external invertase activity in immobilized growing yeast: prolongedSUC2transcription and less susceptibility of the enzyme to endogenous proteolysis

Clues to the origin of high external invertase activity in immobilized growing yeast: prolongedSUC2transcription and less susceptibility of the enzyme to endogenous proteolysis

Staphylococcus carnosus: from starter culture to protein engineering platform

Influence of Different Salinity Levels and Immobilization on Glycerol Production by Halotolerant Yeast Zygosaccharomyces rouxii

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