Continuous production of bacteriocins, brevicin, nisin and pediocin, using calcium alginate‐immobilized bacteria

Wan, Jason; Hickey, M. W.; Coventry, M.J.

doi:10.1111/j.1365-2672.1995.tb00953.x

Cited by 34 publications

(22 citation statements)

References 15 publications

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“…None of the bacteriocins showed activity against Gram-negative bacteria (Aeromonas (Coventry et al 1995 …”

Section: ------------------------------------------------------------mentioning

confidence: 99%

“…the respective indicator lawn culture as described by Wan et al (1995). Bacteriocin activities against the above five indicators were…”

Section: Cultures and Mediamentioning

confidence: 99%

“…appropriate medium, pelleted by centrifugation and stored at Residual bacteriocin activity was determined by the critical −20°C in half the original volume of 10% (v/v) glycerol. dilution method (Wan et al 1995).…”

Section: Preparation Of High Molecular Weight Chromosomal Dna From Bamentioning

confidence: 99%

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Detection of bacteriocins of lactic acid bacteria isolated from foods and comparison with pediocin and nisin

Coventry¹,

Gordon²,

Wilcock³

et al. 1997

Journal of Applied Microbiology

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A .J . H I LL IE R AN D J . WA N. 1997. A total of 663 533 colonies from 72 dairy and meat sources showed a detection rate of 0·2% for bacteriocin producers using direct plating techniques. A further 83 000 colonies from 40 fish and vegetable sources showed a detection rate of 3·4% for bacteriocin producers using selective enrichment procedures. A collection of seven purified isolates showing a different host spectrum of bacteriocin activity and with the ability to produce bacteriocins in broth culture were compared with nisin and pediocin (with respect to their inhibitory activity, determined by the critical dilution method), against various indicator bacteria in agar and broth. The sensitivity of Listeria species to various bacteriocins was influenced by the agar and broth test systems used. A Lactobacillus curvatus strain was found to be the most suitable indicator for quantitating antimicrobial effects of all the bacteriocins investigated in both agar and broth test systems. The bacteriocin-producing isolates were characterized by biochemical reactions and DNA restriction enzyme profiles and taxonomic identification revealed species of Lactobacillus, Carnobacterium and Lactococcus assigned on the basis of 16S rDNA sequences.

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“…None of the bacteriocins showed activity against Gram-negative bacteria (Aeromonas (Coventry et al 1995 …”

Section: ------------------------------------------------------------mentioning

confidence: 99%

“…the respective indicator lawn culture as described by Wan et al (1995). Bacteriocin activities against the above five indicators were…”

Section: Cultures and Mediamentioning

confidence: 99%

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Detection of bacteriocins of lactic acid bacteria isolated from foods and comparison with pediocin and nisin

Coventry¹,

Gordon²,

Wilcock³

et al. 1997

Journal of Applied Microbiology

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“…Various methods are used to determine differences in susceptibility to bacteriocins (6,16,18,38,40,42,47,52,60). To achieve high discrimination between strains with different susceptibilities, a standardized microtiter plate assay, performed with bacteriocin solutions of known concentrations, was used to provide data necessary to determine the potencies of the bacteriocins sakacin P, pediocin PA-1, sakacin A, and nisin against L. monocytogenes.…”

mentioning

confidence: 99%

Differences in Susceptibility of Listeria monocytogenes Strains to Sakacin P, Sakacin A, Pediocin PA-1, and Nisin

Katla

Naterstad²,

Vancanneyt

et al. 2003

Appl Environ Microbiol

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Two hundred strains of Listeria monocytogenes collected from food and the food industry were analyzed for susceptibility to the class IIa bacteriocins sakacin P, sakacin A, and pediocin PA-1 and the class I bacteriocin nisin. The individual 50% inhibitory concentrations (IC 50 ) were determined in a microtiter assay and expressed in nanograms per milliliter. The IC 50 of sakacin P ranged from 0.01 to 0.61 ng ml ؊1 . The corresponding values for pediocin PA-1, sakacin A, and nisin were 0.10 to 7.34, 0.16 to 44.2, and 2.2 to 781 ng ml ؊1 , respectively. The use of a large number of strains and the accuracy of the IC 50 determination revealed patterns not previously described, and for the first time it was shown that the IC 50 of sakacin P divided the L. monocytogenes strains into two distinct groups. Ten strains from each group were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of whole-cell proteins and amplified fragment length polymorphism. The results from these studies essentially confirmed the grouping based on the IC 50 of sakacin P. A high correlation was found between the IC 50 of sakacin P and that of pediocin PA-1 for the 200 strains. Surprisingly, the correlation between the IC 50 of the two class IIa bacteriocins sakacin A and sakacin P was lower than the correlation between the IC 50 of sakacin A and the class I bacteriocin nisin.

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“…Since high biomass density proved to be beneficial for bacteriocin production (Yang and Ray, 1994;Parente and Ricciardi, 1999), it is likely that bacteriocin production could be enhanced in a system with high cell density. Some researchers attempted to increase cell concentration by using immobilized cell reactors, and it was demonstrated that bacteriocin production produced in this reactor was much higher than that of free-cell fermentations (Wan et al, 1995;Bertrand et al, 2001). With the development of membrane technology in the last few years, membrane processes have been increasingly used to separate microbial cells from the culture medium.…”

Section: Introductionmentioning

confidence: 99%

Production of pentocin 31-1 by high cell density Lactobacillus pentosus 31-1 repeated batch cell recycle fermentations

Gui¹,

Li²

2013

Afr. J. Microbiol. Res.

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Pentocin 31-1 was produced by Lactobacillus pentosus 31-1, isolated from the traditional Chinese fermented Xuan-Wei Ham. The wide range of antimicrobial activity especially to Listeria spp showed its potential use as food preservative. The study of pentocin 31-1 production was carried out in repeated batch cell recycle fermentations in 5 L bioreactor and up to 50 L pilot bioreactor. Batch fermentation of L. pentosus 31-1 gave bacteriocin production and productivity of 1395.3 IU/mL and 66.4 IU/(mL h), respectively. The higher bacteriocin production (2186.2 and 2004.5 IU/mL, respectively) and productivity (260.7 and 237.4 IU/(mL h), respectively) was measured in repeated batch cell recycle fermentations in 5 and 50 L bioreactors. Biomass (4.8 and 5.8 g/L, respectively) were also increased with repeated batch cell recycle fermentations in 5 and 50 L bioreactors, which were equivalent to 1.48-and 1.8-fold increases compared with the biomass (3.2 g/L) obtained from batch fermentation. Fermentations were successfully scaled up from 5 to 50 L because there were no significant differences (p<0.05) in production and productivity of bacteriocin between the two volumes by using the same strain and fermentation conditions. These results suggest a reasonable solution to develop large-scale production of both pentocin 31-1 and L. pentosus 31-1 in food industry.

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Continuous production of bacteriocins, brevicin, nisin and pediocin, using calcium alginate‐immobilized bacteria

Cited by 34 publications

References 15 publications

Detection of bacteriocins of lactic acid bacteria isolated from foods and comparison with pediocin and nisin

Detection of bacteriocins of lactic acid bacteria isolated from foods and comparison with pediocin and nisin

Differences in Susceptibility of Listeria monocytogenes Strains to Sakacin P, Sakacin A, Pediocin PA-1, and Nisin

Production of pentocin 31-1 by high cell density Lactobacillus pentosus 31-1 repeated batch cell recycle fermentations

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