Long-term culture of embryonic rat cardiomyocytes on an organosilane surface in a serum-free medium

A defined system to allow skeletal muscle differentiation and subsequent integration with silicon microstructures

“…It has been shown that biological molecules can be incorporated into SAMs through crosslinkers, which could also enable selective study of specific contact signaling pathways [19]. SAMcoated surfaces have also been used to grow and pattern hippocampal neurons, adult spinal cord neurons, motoneurons, cardiomyocytes, endothelial cells and muscle cell lines [2][3][4]15,[19][20][21][22].…”

Isolation and Culture of Neonatal Mouse Cardiomyocytes

“…matrigel 25,26 ) or artificial substrates (i.e. silicone polymer 27 , organosilane 28 ) positively influences cardiomyocyte adherence, cell viability and cell spreading during the initial plating step and for the subsequent culture period. Particularly the culture of cardiomyocytes on glass substrates requires the coating of the surface with ECM components to achieve proper adherence of cardiomyocytes.…”

Fetal bovine serum (FBS): Past – present – future

“…Global Biological Resource Centres (BRCs) are equipped with authentication standards to ensure an optimal use of these models (Almeida et al, 2016;Barallon et al, 2010;Capes-Davis and Neve, 2016;Freedman et al, 2015). A recent survey revealed a reproducibility crisis in biomedical science, since more than 70% of researchers have failed to reproduce published experiments (Baker, 2016a), which in cancer research can be partly attributed to the use of different batches of FBS, as will be discussed below. Furthermore, the fast-growing knowledge from intra-tumor across inter-patient to intra-cell line heterogeneities demands a comprehensive maintenance and safeguarding of a sufficient number of models to represent the genomic diversity observed across human cancers.…”

“…There is thus a clear need for the replacement of FBS and for the design of serum-free cell culture media, respectively, which will result in better standardization and data reproducibility of in vitro applications (Gstraunthaler et al, 2014) and will substantially contribute to GCCP (Coecke et al, 2005). Due to the large commercial interest in providing FBS, fraudulent practices have become apparent, which will have an effect on, among other issues, reproducibility, adding to the reproducibility crisis (Baker, 2016a). The use of FBS in Biological Resource Centers is also becoming problematic since changes in the phenotype and genotype of cell lines when changing to new FBS batches might not always be obvious.…”

“…This was extended to create the first defined system where all the medium components, including the culture vessel surface modifications, in addition to the medium were well-defined (Schaffner et al, 1995). This technology has now been extended to include cardiomyocytes (Das et al, 2004; Natara-the polar architecture and junctional assembly of the epithelial cells, presence of vectorial transport, sidedness of cellular uptake routes, and expression of kidney-specific metabolic and transport properties. The generation of transepithelial electrical resistance (TEER) can be determined as an overall parameter for the integrity of the cultured epithelium (Srinivasan et al, 2015).…”