Long noncoding RNA neuroblastoma-associated transcript 1 gene inhibits malignant cellular phenotypes of bladder cancer through miR-21/SOCS6 axis

Abstract

Bladder cancer (BC) is one of the most common tumors in the urinary system. Noncoding RNAs are considered to take part in cellular phenotypes and are emerging as diagnostic and prognostic biomarkers of BC. The aim of this study is to investigate the clinical significance of neuroblastoma- associated transcript 1 (NBAT1) gene and its effects on malignant cellular phenotypes in BC. NBAT1 gene was low-expressed in BC tissues and cell lines and its low-expression was related with high pathological grade and metastasis of BC. Upregulation of NBAT1 gene depressed cell viability and invasiveness of KK47 and T24 cells and arrested KK47 and T24 cells at G1 stage. In addition, NBAT1 could target silence the expression of miR-21-5p in RNA-induced silencing complex-dependent manner. KK47 and T24 cells with miR-21-5p knockdown showed reduced cell viability, G1-stage arrest, and depressed invasiveness. MiR-21-5p mediates the regulatory effects of NBAT1 on malignant cellular phenotypes of BC cells. Moreover, SOCS6 gene was a target gene of miR-21-5p, and miR-21-5p modulated malignant cellular phenotypes of KK47 and T24 cells through targeted silencing of SOCS6. In conclusion, low-expression of NBAT1 is associated with the progress and metastasis of BC, and NBAT1 inhibits malignant cellular phenotypes through miR-21-5p/SOCS6 axis in BC. Our findings help to elucidate the tumorigenesis of BC, and future study will provide a novel therapeutic target for BC.

Abnormal expression of long noncoding RNA FGD5-AS1 affects the development of periodontitis through regulating miR-142-3p/SOCS6/NF-κB pathway

“…SOCS6 is a member of SOCS family proteins that are known as regulators of inflammatory responses induced by bacterial infections [34]. Also, SOCS6 is identified as a cytokine-inducible negative regulator of cytokine signalling [35] and is involved in negative regulation of receptor signalling and inducing apoptosis by targeting mitochondrial proteins [36]. Evidence also points out that SOCS6 is implicated in diverse human cancers [37,38].…”

Long non-coding RNA FOXF1 adjacent non-coding developmental regulatory RNA inhibits growth and chemotherapy resistance in non-small cell lung cancer

“…LncRNAs are hotspots in current research, in which anomalous expression of certain lncRNAs is found in a variety of malignant cancers, and those lncRNAs can act as tumor-suppressing genes or oncogenes to play a part in genesis and progression of tumors [12,13]. It is well known that ln-cRNAs possess various functions and are involved in almost all cancer cell characteristics, including apoptosis, invasion, autophagy and chemotherapy resistance [14][15][16][17][18][19].…”

Expression of long noncoding RNA NBAT1 is associated with the outcome of patients with non-small cell lung cancer

“…LncRNA neuroblastoma associated transcript 1 (NBAT1), also known as CASC14, was located at 6p22.3 and was initially identified to be dysregulated in neuroblastoma 16 . Then, several studies reported that NBAT1 expression was down-regulated in several tumors, such as bladder cancer, glioblastoma, osteosarcoma [17][18][19] . In addition, both in vitro and in vivo assays confirmed the tumor-suppressive roles of NBAT1 in the above tumors.…”