Long non‑coding RNA LINC00473/miR‑195‑5p promotes glioma progression via YAP1‑TEAD1‑Hippo signaling

Abstract

There is an urgent need to identify novel potential therapeutic targets for diagnosis and treatment of glioma, a common primary tumor in brain, due to its high-level invasiveness. Long non-coding RNA (lncRNA) LINC00473 has been reported as potentially critical regulators in various types of cancer tumorigenesis. However, the effects of LINC00473 on glioma cells is unclear. The present study aimed to investigate the clinical significance, effects and mechanism of LINC00437 on glioma tumorigenesis. In the present study, LINC00473 was significantly increased in glioma tissues and in cell models, and predicted a poor prognosis in patients with glioma. Notably, LINC00473 knockdown not only suppressed cell proliferation, invasion and migration of glioma cells, but also blocked cell cycle progression and induced apoptosis. Subcutaneous xenotransplanted tumor model experiments revealed that reduced LINC00473 expression was able to suppress in vivo glioma growth. Mechanistically, LINC00473 functioned as a competing endogenous (ce)RNA to decrease microRNA (miR)-195-5p expression. Moreover, Yes-associated protein 1 (YAP1) and TEA domain family member 1 (TEAD1) were identified as downstream targets of miR-195-5p, whose expression levels were inhibited by miR-195-5p. LINC00473 knockdown suppressed glioma progression through the decrease of miR-195-5p and subsequent increase of YAP1 and TEAD1 expression levels. These results indicated LINC00473 might act as a ceRNA to sponge miR-195-5p, thus promoting YAP1 and TEAD1 expressions, and shedding light on the underlying mechanisms of LINC00473-induced glioma progression.

<p>lncRNA CRNDE is Upregulated in Glioblastoma Multiforme and Facilitates Cancer Progression Through Targeting miR-337-3p and ELMOD2 Axis</p>

“… 14 High lncRNA LINC00473 was identified as a predictor for worse survival of glioma patients. 15 They also found LINC00473 regulates glioma progression by inhibiting miR-195-5p and activate Yes‑associated protein 1-EA domain family member 1-Hippo signaling pathway. 15 In this study, CRNDE was found highly expressed in GBM tissues and cells.…”

“… 15 They also found LINC00473 regulates glioma progression by inhibiting miR-195-5p and activate Yes‑associated protein 1-EA domain family member 1-Hippo signaling pathway. 15 In this study, CRNDE was found highly expressed in GBM tissues and cells. Besides, high CRNDE was significantly correlated with poorer overall survival of GBM patients.…”

Long non‑coding RNA AFAP1‑AS1 facilitates the growth and invasiveness of oral squamous cell carcinoma by regulating the miR‑145/HOXA1 axis

“…IHC was performed to analyze HOXA1 expression levels in xenograft tumors were assessed according to a previous study ( 21 ). Briefly, the tumor tissues were dissected and fixed in 4% paraformaldehyde at 25°C overnight, embedded in paraffin and then dissected into sections (5 µm), deparaffinized in xylene, rehydrated with graded alcohols (100, 90, 70 and 50%).…”

LINC00473: A novel oncogenic long noncoding RNA in human cancers

“…Relative to normal brain tissues, LINC00473 expression in glioma tissues is significantly higher (X. Wang et al, 2020;Q. Zhang et al, 2019).…”