<p>lncRNA CRNDE is Upregulated in Glioblastoma Multiforme and Facilitates Cancer Progression Through Targeting miR-337-3p and ELMOD2 Axis</p>

Abstract

Introduction: Colorectal neoplasia differentially expressed (CRNDE) was reported to promote carcinogenesis in several cancers. However, the role of CRNDE in glioblastoma multiforme (GBM) needs to be further explored. Methods: CRNDE expression levels in GBM tissues and cells were explored using realtime quantitative PCR at first. Effects of CRNDE on GBM cell behaviors were detected by conducting in vitro experiments. Interactions of CRNDE, microRNA-337-3p (miR-337-3p), and ELMO domain containing 2 (ELMOD2) were verified by bioinformatics analysis tools and dual-luciferase reporter assay. Expression correlations of CRNDE and ELMOD2 in GBM tissues were analyzed at GEPIA website. Results: CRNDE expression was upregulated in GBM tissues and cells compared with normal counterparts. CRDNE knockdown inhibits proliferation and migration, but promotes apoptosis in GBM cell, while CRNDE overexpression caused opposite effects. Mechanisms exploration indicated CRNDE serves as sponge of miR-337-3p to upregulate ELMOD2 expression. Furthermore, we showed CRNDE and ELMOD2 were positively correlated in GBM tissues. Discussion: In conclusion, our study highlighted the importance of CRNDE/miR-337-3p/ ELMOD2 axis in GBM progression and offered novel strategies for GBM treatment.

LncRNA MIR205HG Drives Esophageal Squamous Cell Carcinoma Progression by Regulating miR-214/SOX4 Axis

“…Recently, global transcriptional analyses have identified that mammalian genomes contain numerous long non-coding RNA (lncRNA), which is defined as a class of non-coding RNAs whose length is longer than 200 nt. 4 , 5 Existing researches revealed that lncRNA could serve as the biomarker for prognosis and diagnosis in multiple cancers. 6 , 7 Notably, studies over the past years have demonstrated that lncRNAs are frequently dysregulated and play a pivotal role in tumor metastasis, including ESCC.…”

LncRNA CRNDE exacerbates neuropathic pain in chronic constriction injury-induced(CCI) rats through regulating miR-146a-5p/WNT5A pathway

“…Numerous studies have confirmed that lncRNAs play a crucial role in the etiopathogenesis of NP [30][31][32]. CRNDE has been extensively investigated in several malignant tumors and has been identified as a tumor promoting factor [33][34][35][36]. Furthermore, many studies have confirmed that CRNDE is involved in the regulation of neurobehavioral functions [37][38][39].…”

Large-Scale Transcriptomics-Driven Approach Revealed Overexpression of CRNDE as a Poor Survival Prognosis Biomarker in Glioblastoma

“…We then intersected the gene sets obtained and found only one common gene that was differentially regulated (overexpressed) in all four datasets ( Figure 4 and Figure 5 , Supplementary Table S2 ). This was the gene for noncoding RNA CRNDE (ColoRectal Neoplasia Differentially Expressed) that was previously associated with many cancers [ 41 , 42 ] including glioblastoma [ 43 , 44 ].…”

“…We then intersected the gene sets obtained and found only one common gene that was differentially regulated (overexpressed) in all four datasets (Figures 4 and 5, Supplementary Table S2). This was the gene for noncoding RNA CRNDE (ColoRectal Neoplasia Differentially Expressed) that was previously associated with many cancers [41,42] including glioblastoma [43,44]. To test if a given number of common differential genes or pathways between intersecting datasets is statistically significant, perturbation test for randomness was performed according to [33] (see Materials and Methods).…”