LncRNA MIR205HG Drives Esophageal Squamous Cell Carcinoma Progression by Regulating miR-214/SOX4 Axis

Abstract

Background Esophageal squamous cell carcinoma (ESCC) is a common and fatal malignancy, which has posed a great challenge to public health, especially in China. Dysregulation of long non-coding RNAs is involved in the occurrence, development, invasion, and metastasis of multiple cancers including ESCC. However, little is known about the function of MIR205HG in ESCC. Methods We used qRT-PCR to detect the expression level of MIR205HG, miR-214, and SOX4 in human ESCC tissues and cell lines. Loss-of-functional assays were performed to test the impact of MIR205HG on cell proliferation, metastasis, and apoptosis process via CCK-8, transwell, and flow cell cytometry assays. Additionally, the downstream molecular mechanism of MIR205HG in ESCC was explored. Results Here, we found MIR205HG was substantially up-regulated in ESCC, and there was a positive correlation between MIR205HG expression and tumor size and lymphatic metastasis of ESCC patients. Inhibition of MIR205HG attenuated cell proliferation, migration, and invasion. Silencing MIR205HG increased G1 phase cell counts and decreased S phase cell counts, along with increased apoptotic cell populations. Notably, the rescue assays indicated that miR-214 could partly reverse the influence of MIR205HG on ESCC cell migration. We also found that SOX4 was a direct target mRNA of miR-214, and MIR205HG could act as a molecular sponge to regulate SOX4 expression in ESCC. Conclusion Taken together, our findings demonstrate that MIR205HG promotes ESCC progression by regulating the miR-214/SOX4 axis. MIR205HG may be a novel candidate target for ESCC diagnosis and therapy.

LncRNA MIR205HG accelerates cell proliferation, migration and invasion in hepatoblastoma through the activation of MAPK signaling pathway and PI3K/AKT signaling pathway

“…It is implicated that MIR205HG widely participates in diverse types of cancers. For example, Li et al have certified that MIR205HG aggravates the process of esophageal cell carcinoma through sponging miR-214 and regulating SOX4 expression ( 14 ). Liu et al have identified that MIR205HG acts as an oncogene in lung squamous cell carcinoma [ 15 ].…”

“…As a common lncRNA, MIR205HG has been also investigated in multiple malignancies, including esophageal cell carcinoma, lung squamous cell carcinoma, cervical cancer and so on [ 14 – 16 ]. In this study, we probed into the effects of MIR205HG on the progression of HB and investigated into the latent regulatory mechanism.…”

Identification and Validation of Three Autophagy-Related Long Noncoding RNAs as Prognostic Signature in Cholangiocarcinoma

“…Interestingly, CHRM3.AS2 was a risk-associated gene in our study, which was highly expressed in CCA. Moreover, as for MIR205HG, Li et al demonstrated that MIR205HG could exert roles on cell cycle, migration, and apoptosis of esophageal squamous cell carcinoma by mediating miR-214 negatively and regulating SOX4 as a molecular sponge, which can be regarded as a novel candidate for the diagnosis and treatment of that tumor (40). In another recent study, Liu and colleagues demonstrated that by acting as a competing endogenous RNA, MIR205HG could accelerate lung squamous cell carcinoma development via mediating the molecular axis of miR-299-3p/MAP 3 K2 (41).…”

MIR205 host gene (MIR205HG) drives osteosarcoma metastasis via regulating the microRNA 2114-3p (miR-2114-3p)/twist family bHLH transcription factor 2 (TWIST2) axis

“…Research shows that MIR205HG functions as a fundamental player in various human cancer by affecting different pathways in a context-dependent manner [ 15 , 16 ]. In this work, the biological role of MIR205HG in OS was characterized.…”