Loganin attenuates intestinal injury in severely burned rats by regulating the toll‑like receptor 4/NF‑κB signaling pathway

Abstract

Severe burns may lead to intestinal inflammation and oxidative stress, resulting in intestinal barrier damage and gut dysfunction. Loganin, an iridoid glycoside compound, has been isolated from Cornus officinalis Sieb. et Zucc; however, its role in the treatment of burn injury is yet to be fully elucidated. Therefore, the present study examined the effect of loganin administration on burn-induced intestinal inflammation and oxidative stress after severe burns in male Sprague-Dawley rats. Histological injury was assessed by hematoxylin and eosin staining. Furthermore, cytokine expression in intestinal tissues was measured by ELISA and reverse transcription-quantitative PCR. Antioxidative activities were assessed by determining the levels of reactive oxygen species (ROS), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) and malondialdehyde (MDA). Apoptosis was detected by flow cytometry. Apoptosis-related proteins, toll-like receptor 4 (TLR4) protein and NF-κB translocation were examined by western blotting. Immunohistochemical staining was used to observe TLR4 and NF-κB p65 expression in intestinal tissues. The present study suggested that loganin administration significantly reduced burn injury-induced intestinal histological changes, tumor necrosis factor-α, interleukin (IL)-6 and IL-1β production and oxidative stress, evidenced by decreased ROS levels and MDA content (P<0.05). Furthermore, loganin increased SOD, CAT and GSH-Px levels and intestinal epithelial cell apoptosis. Loganin treatment also significantly inhibited activation of the TLR4/NF-κB signaling pathway in the intestine of severely burned rats (P<0.05). In conclusion, loganin reduced burns-induced intestinal inflammation and oxidative stress, potentially by regulating the TLR4/NF-κB signaling pathway.

Loganin Inhibits Lipopolysaccharide-Induced Inflammation and Oxidative Response through the Activation of the Nrf2/HO-1 Signaling Pathway in RAW264.7 Macrophages

“…2,10) According to our results, loganin strongly inhibited LPS-induced ROS formation in RAW264.7 macrophages. Although the antioxidant potential of loganin has been reported in previous studies, 18,25,26) the results of this study support the use of loganin as an antioxidant for the management of oxidative stress associated with inflammatory responses. Certain antioxidant signaling pathways negatively modulate excessive inflammatory responses to maintain homeostasis in the body.…”

“…[14][15][16] A number of previous studies have shown that loganin has a variety of beneficial pharmacological effects, including anti-inflammatory, antioxidant, neuroprotective, immune regulatory and anti-tumor effects. [17][18][19][20][21][22][23][24] Recently, Cheng et al 25) reported that loganin can ameliorate pyroptosis, an inflammatory programmed cell death, by mitigating oxidative stress through inhibition of ROS generation and inactivation of NF-κB in high glucose-stimulated Schwann cells. In addition, it has been proposed that the effectiveness on anti-inflammation and antioxidant of loganin could be mediated by modulating the TLR4/NF-κB signaling pathway in an experimental model of loganine burn injury, 26) and the inhibition of TLR4/NF-κB activity was also attributed to suppress the inflammatory response upon LPS in the intestinal epithelium.…”

The Herba Patriniae (Caprifoliaceae): A review on traditional uses, phytochemistry, pharmacology and quality control

“…Of these, loganin (131), loganic acid (132) and morroniside (136) are the compounds with more reported biological activities. Loganin has been proved with inhabitation effect on inflammatory response ( Wen et al, 2020 ; Wei et al, 2013a ) and mitigative effect on osteoarthritis ( Hu et al, 2020 ). Morroniside has shown protective effect against H 2 O 2 -induced damage in human neuroblastoma cells ( Zhong et al, 2017 ).…”

Loganin Inhibits Angiotensin II–Induced Cardiac Hypertrophy Through the JAK2/STAT3 and NF-κB Signaling Pathways

“…Similarly, Shirin and colleagues revealed that acute administration of loganin could enhance spatial memory in diabetic rats (Babri et al, 2013). Moreover, Wen et al (2020) illustrated that loganin could ameliorate burn injuries and significantly reduce the production of inflammatory cytokines and oxidative stress by repressing the activation of the TLR4/NF-κB signaling pathway. To the best of our knowledge, only one study has implied that loganin appears to suppress the progression of atherosclerosis (Li et al, 2016).…”