Location of monoclonal antibody binding sites in the capsid protein of feline calicivirus

Structural, antigenic and immunogenic relationships between European brown hare syndrome virus and rabbit haemorrhagic disease virus.

“…The latter part of the protein would be exposed and could be relevant to antigenic variability. Consistent with this prediction is the mapping of two neutralizing epitopes of feline calicivirus in the C-terminal half of the capsid protein (Milton et al, 1992). A highly divergent domain (33 % identity) of 27 amino acids (residues 407-434) that might be responsible for antigenic variability was found within the C-terminal region of the EBHSV and RHDV proteins.…”

“…The virion is 35 nm in diameter and shows a structured surface with regularly arranged cup-shaped depressions . Within the Caliciviridae, RHDV and EBHSV are unusual in that the coding sequence of their capsid gene is part of the first ORF, which encodes a large polyprotein, whereas the capsid protein of the other caliciviruses is encoded by a distinct ORF (Jiang et al, 1990 ;Meyers et al, 1991 b ;Carter et al, 1992 ;Dingle et al, 1995). A subgenomic RNA of 2n2 kb, which is collinear with the 3h terminus of the genome and covers the capsid gene, is found in both viruses Wirblich et al, 1994).…”

Analysis of feline calicivirus capsid protein genes: identification of variable antigenic determinant regions of the protein

“…One of the MAbs produced by Milton et al (1992), 4E7, crossreactive with the CFI/68 isolate, bound the hypervariable region between residues 422 to 448. Another MAb, 1Gg, not cross-reactive with CFI/68, bound to residues 449 to 458 where the greatest amount of sequence divergence occurred between the F9 and CFI/68 capsid proteins (Milton et al, 1992). These are predicted hydrophilic areas (Kyte & Doolittle, 1982) of the protein exhibiting a high antigenic index (Jameson & Wolf, 1988).…”

“…The F9 calicivirus capsid protein E region has been expressed (Guiver et al, 1992;Milton et al, 1992) and MAbs produced against this region were neutralizing (Toya et al, 1991a;Milton et al, 1992). One of the MAbs produced by Milton et al (1992), 4E7, crossreactive with the CFI/68 isolate, bound the hypervariable region between residues 422 to 448.…”

Mapping of antigenic sites involved in neutralization on the capsid protein of feline calicivirus.

“…The capsid of FCV is considered to play an important role in the antigenic variability of the virus because FCV has only a single capsid protein (Bachrach & Hess, 1973 ;Burroughs & Brown, 1974). Studies on expression and antigenic analysis of the capsid protein using prokaryotic expression systems have revealed that at least one major neutralizing epitope exists in the region between amino acids 408 and 517 (Guiver et al, 1992) and that two neutralizing monoclonal antibodies (MAbs) bound to a region of 37 amino acids between positions 422 and 458 (Milton et al, 1992). Recently, variation in the capsid protein gene among FCV isolates has been established by comparative sequence studies Seal et al, 1993 ;Seal, 1994).…”