Analysis of feline calicivirus capsid protein genes: identification of variable antigenic determinant regions of the protein

Seal, Bruce S.; Ridpath, Julia F.; Mengeling, W. L.

doi:10.1099/0022-1317-74-11-2519

Cited by 94 publications

(79 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The apparent molecular weight of the capsid protein of CaCV No. 48 strain was similar to that of the CaCV described by Schaffer et al [13], RHDV [10] and NV [7] but slightly less than that reported for FCV [1,15,18]. The time course study of capsid protein synthesis revealed that the protein band was already detectable by the mouse serum at 2 hr PI.…”

Section: Discussionsupporting

confidence: 49%

Identification of Canine Calicivirus Capsid Protein and Its Immunoreactivity in Western Blotting.

Gabriel

Tohya

Sugimura

et al. 1997

The Journal of Veterinary Medical Science

View full text Add to dashboard Cite

ABSTRACT. A canine calicivirus (CaCV) isolated in Japan, designated as CaCV No. 48 strain, was propagated in MDCK cells and purified by CsCl equilibrium gradient centrifugation. Sodium dodecyl sulfate-polyacrylamide gel electrophoretic analysis of the purified samples revealed the presence of only one major species of viral protein of about 60 kilodaltons after Coomassie staining. The same band, presumably that of the capsid protein, was detected by western blotting using a mouse hyperimmune serum. This capsid protein was synthesized in MDCK cells as early as 2 hr post-inoculation. Experimental infection of dogs resulted in the production of anti-CaCV antibodies which were detected by microneutralization test and western blotting. Likewise, serosurvey revealed not only the presence of neutralizing antibodies but also reactivity of the field sera against the capsid protein of the purified virus. These results indicate that the capsid protein of CaCV No. 48 strain is immunogenic and could be detected by antibodies in western blotting. -KEY WORDS: canine calicivirus, capsid protein, western blotting.

show abstract

Section: Discussionsupporting

confidence: 49%

Identification of Canine Calicivirus Capsid Protein and Its Immunoreactivity in Western Blotting.

Gabriel

Tohya

Sugimura

et al. 1997

The Journal of Veterinary Medical Science

View full text Add to dashboard Cite

show abstract

“…This polyprotein is posttranslationally cleaved by the viral protease. ORF 2 codes for the major capsid protein which has been divided in to six regions A-F based largely on sequence conservation [99]. Region B, D and F are relatively conserved between FCV isolates, whilst regions C and E are variable.…”

Section: Aetiologymentioning

confidence: 99%

Feline calicivirus

et al. 2007

View full text Add to dashboard Cite

-Feline calicivirus (FCV) is an important and highly prevalent pathogen of cats. It belongs to the family Caliciviridae which includes other significant pathogens of man and animals. As an RNA virus, high polymerase error rates convey upon FCV a high genome plasticity, and allow the virus to respond rapidly to environmental selection pressures. This makes the virus very adaptable and has important implications for clinical disease and its control. Being genetically diverse, FCV is associated with a range of clinical syndromes from inapparent infections to relatively mild oral and upper respiratory tract disease with or without acute lameness. More recently, highly virulent forms of the virus have emerged associated with a systemic infection that is frequently fatal. A proportion of FCV infected cats that recover from acute disease, remain persistently infected. In such cats, virus evolution is believed to help the virus to evade the host immune response. Such longterm carriers may only represent a minority of the feline population but are likely to be crucial to the epidemiology of the virus. Vaccination against FCV has been available for many years and has effectively reduced the incidence of clinical disease. However, the vaccines do not prevent infection and vaccinated cats can still become persistently infected. In addition, FCV strain variability means that not all strains are protected against equally. Much progress has been made in understanding the biology and pathogenesis of this important feline virus. Challenges for the future will necessarily focus on how to control the variability of this virus particularly in relation to emerging virulent strains and vaccination.

show abstract

“…Fax +44 1223 336926. e-mail tdkb@mole.bio.cam.ac.uk mately 12 kDa. The predicted protein shows 90-94 % identity at the amino acid sequence level amongst five different FCV strains (Seal et al, 1993). A homologous small ORF is also present at the 3" end of NV, RHDV, VESV and SMSV (Carter, 1990;Meyers et al, t991;Neill, 1992;Jiang et al, 1993) and is conserved at the amino acid sequence level within members (Milton et al, 1992;Neill, 1992;Seal et al, 1993).…”

mentioning

confidence: 99%

Detection of the ORF3 polypeptide of feline calicivirus in infected cells and evidence for its expression from a single, functionally bicistronic, subgenomic mRNA

Herbert

Brierley

Brown

1996

Journal of General Virology

View full text Add to dashboard Cite

Analysis of feline calicivirus capsid protein genes: identification of variable antigenic determinant regions of the protein

Cited by 94 publications

References 43 publications

Identification of Canine Calicivirus Capsid Protein and Its Immunoreactivity in Western Blotting.

Identification of Canine Calicivirus Capsid Protein and Its Immunoreactivity in Western Blotting.

Feline calicivirus

Detection of the ORF3 polypeptide of feline calicivirus in infected cells and evidence for its expression from a single, functionally bicistronic, subgenomic mRNA

Contact Info

Product

Resources

About