Mapping of antigenic sites involved in neutralization on the capsid protein of feline calicivirus.

Tohya, Yukinobu; Yokoyama, Naoki; Maeda, Ken; Kawaguchi, Yasushi; Mikami, Takeshi

doi:10.1099/0022-1317-78-2-303

Cited by 69 publications

(68 citation statements)

References 15 publications

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“…Region B, D and F are relatively conserved between FCV isolates, whilst regions C and E are variable. Variable region E is known to contain the major B-cell epitopes [32,86,110] and its variability has been used as the basis of sequence-based methods to differentiate between strains [84,105]. Region A is cleaved to produce the mature capsid protein [10,102].…”

Section: Aetiologymentioning

confidence: 99%

Feline calicivirus

et al. 2007

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-Feline calicivirus (FCV) is an important and highly prevalent pathogen of cats. It belongs to the family Caliciviridae which includes other significant pathogens of man and animals. As an RNA virus, high polymerase error rates convey upon FCV a high genome plasticity, and allow the virus to respond rapidly to environmental selection pressures. This makes the virus very adaptable and has important implications for clinical disease and its control. Being genetically diverse, FCV is associated with a range of clinical syndromes from inapparent infections to relatively mild oral and upper respiratory tract disease with or without acute lameness. More recently, highly virulent forms of the virus have emerged associated with a systemic infection that is frequently fatal. A proportion of FCV infected cats that recover from acute disease, remain persistently infected. In such cats, virus evolution is believed to help the virus to evade the host immune response. Such longterm carriers may only represent a minority of the feline population but are likely to be crucial to the epidemiology of the virus. Vaccination against FCV has been available for many years and has effectively reduced the incidence of clinical disease. However, the vaccines do not prevent infection and vaccinated cats can still become persistently infected. In addition, FCV strain variability means that not all strains are protected against equally. Much progress has been made in understanding the biology and pathogenesis of this important feline virus. Challenges for the future will necessarily focus on how to control the variability of this virus particularly in relation to emerging virulent strains and vaccination.

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Section: Aetiologymentioning

confidence: 99%

Feline calicivirus

et al. 2007

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“…1,11,13,32 It contains at least 1 neutralizing epitope. 12,40 The ORF3, which overlaps ORF2, (nucleotides 7617-7626 to 7634-7643), encodes a minor structural protein, VP2, with an unknown function. 15,22,34 Virus-neutralization tests indicate that FCV isolates are closely related to one another and comprise 1 serotype.…”

Section: Introductionmentioning

confidence: 99%

Genetic Analysis of Feline Caliciviruses Associated with a Hemorrhagic-Like Disease

2005

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Abstract. Feline calicivirus (FCV) is 1 of the most common causes of upper respiratory tract disease in cats. Other disease syndromes associated with FCV infection have been reported. Recently, calicivirus infection associated with a hemorrhagic-like disease leading to significant mortality in cats has been reported. The clinical signs are similar to those observed with the calicivirus of rabbit hemorrhagic disease. This study characterized 2 FCV isolates associated with hemorrhagic-like disease. Nucleotide sequencing of the complete genome has been done for these 2 isolates as well as for 4 additional isolates representing other disease syndromes. Previously reported sequence data for the entire genome of classical FCV (6 isolates) and a portion of the capsid gene for hemorrhagic-like FCV (3 isolates), isolated in different regions of United States were used in the genetic analysis. Sequence data were used to determine relationships among the isolates and any correlation with phenotype. Nucleotide sequence comparisons of the entire genome and individual open reading frames revealed high homology among all isolates. Data suggest that the virulence may have genetic determinants on the basis of phylogenetic clustering of the isolates associated with hemorrhagic-like disease.

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“…These two MAbs recognized Feline calicivirus (FCV) is a causative agent of acute oral and upper respiratory diseases in cats [2,5]. Previously, Tohya et al established a number of neutralizing monoclonal antibodies (MAbs) against the F4 strain of FCV, the prototype strain of FCV in Japan, and identified seven neutralizing epitopes [11,12]. Recently, we performed neutralization tests (NT) of these MAbs with FCV field isolates and laboratory strains, and classified the viruses into 4 groups according to their reactivities [8].…”

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confidence: 99%

“…These MAbs were produced previously and confirmed to recognize neutralizing epitope on the virus capsid protein by antigenic analysis in the expression system [6,11,12]. In the present study, ascitic fluids of mice were used as antibodies.…”

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confidence: 99%

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Reactivities of Feline Calicivirus Field Isolates with Monoclonal Antibodies Detected by Enzyme-Linked Immunosorbent Assay

Tajima

Takeda

Tohya

et al. 1998

The Journal of Veterinary Medical Science

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ABSTRACT. Reactivities of feline calicivirus (FCV) field isolates with monoclonal antibodies (MAbs) were examined by enzyme-linked immunosorbent assay (ELISA). The reactivities of the viruses in ELISA were different from our previous results using the neutralization tests (NT). Many isolates were positive in ELISA with MAbs which recognized neutralizing epitope 3B and/or 4. However, most were negative in NT in our previous study. After absorption of two FCV strains with host cells, the non-infectious virus fluid still reacted with MAb, which recognized epitope 3B and/or 4 in ELISA. These results indicated the possibility that neutralizing epitopes are expressed on non-infectious virus particles or exist as proteinaceous molecules in virus fluid. -KEY WORDS: ELISA, feline calicivirus, monoclonal antibody.J. Vet. Med. Sci. 60(6): 753-755, 1998 predetermined concentration (1:1,000) of conjugate was reacted. The colorimetric reaction was performed by addition of 0.2 mM 2,2-azinobis(3-ethylbenzthiazolinesulfonic acid) and 0.004% H 2 O 2 in 0.05 M citrate buffer, pH 4.0, as a substrate. As a negative control, ascitic fluid from mice inoculated with a mouse myeloma cell line, SP2/ O, was used. The absorbances at 405 nm of the MAbreacted wells were subtracted from those of the SP2/O ascitic fluid-reacted wells. If the subtracted absorbance was higher than 0.1, it was considered positive. The ELISA titer was expressed by the reciprocal of the highest dilution that showed positive reaction. The test was performed twice and geometric means were calculated. In order to compare the results, r value is calculated by using the formula of r= antibody titer against test strain antibody titer against F4 strain. According to the 20-unit concept [1,4], r value higher than 0.05 was considered as positive.

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Mapping of antigenic sites involved in neutralization on the capsid protein of feline calicivirus.

Cited by 69 publications

References 15 publications

Feline calicivirus

Feline calicivirus

Genetic Analysis of Feline Caliciviruses Associated with a Hemorrhagic-Like Disease

Reactivities of Feline Calicivirus Field Isolates with Monoclonal Antibodies Detected by Enzyme-Linked Immunosorbent Assay

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