Localization of telocytes in rabbits testis: Histological and immunohistochemical approach

Awad, Mahmoud; Ghanem, Mohamed Elshabrawy

doi:10.1002/jemt.23133

Cited by 11 publications

(6 citation statements)

References 35 publications

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“…TCs identification was based on morphological criteria, using different techniques; SEM, TEM, and immunophenotyping. TCs have been identified in the mammalian testis of human 37 , mouse 38 , rabbit 39 . Camel TCs possessed features identical to those of TCs in other mammalian species 40–43 .…”

Section: Discussionmentioning

confidence: 99%

Morphological changes of telocytes in camel efferent ductules in response to seasonal variations during the reproductive cycle

Abdel-Maksoud

Abd‐Elhafeez

Soliman

2019

Sci Rep

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Telocytes (TCs) are a distinct stromal cell type described in many organs. The present study investigated the existence of TCs within the efferent ductules in camel and the changes that occur in their morphology and activity during active and inactive reproductive seasons. TCs in the camel had a cell body and multiple telopodes (TPs), and most TCs had indented nuclei that exhibited prominent intranucleolar chromatin. TCs exhibited seasonal differences which were evaluated by histochemistry, immunohistochemistry (IHC), Transimition electron microscopy (TEM) and scanning electron microscopy (SEM). The presence of TCs in camel efferent ductules has been confirmed by CD34 positive immunostaing. In addition to the expression of the vascular endothelial growth factor (VEGF) which was stronger in the summer season. TCs exhibited stronger immunoreactivity for progesterone and oestrogen alpha receptors (ESR1) in the spring than in the summer. In addition, TCs showed strong positive immunostaining for both vimentin and androgen receptor (AR). Several ultrastructural changes were observed in TCs during the two seasons. TPs in the summer season had delicate ramifications whereas, in the spring, TPs displayed fine arborization and became more corrugated. TCs acquired signs of exaggerated secretory activities in the spring; TPs became expanded and packed with secretory vesicles. Thus, we conclude that, hormonal alterations during the reproductive cycle impact the morphology and secretory behavior of TCs.

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Section: Discussionmentioning

confidence: 99%

Morphological changes of telocytes in camel efferent ductules in response to seasonal variations during the reproductive cycle

Abdel-Maksoud

Abd‐Elhafeez

Soliman

2019

Sci Rep

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“…In addition to the rat, telocytes have been found in the testes of several species, with slight variations in their presence and interrelationships. Histologically, telocytes formed a sheath surrounding the seminiferous tubules, as well as being observed in the interstitium in rabbits (Awad & Ghanem, 2018). In doves, telocytes are located in the peritubular region, specifically close to blood vessels.…”

Section: Male Reproductive Systemmentioning

confidence: 99%

Telocytes of the male urogenital system: Interrelationships, possible functions, and pathological implications

Sanches

Tamarindo

Maldarine

et al. 2021

Cell Biology International

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The male urogenital system is composed of the reproductive system and the urinary tract; they have an interconnected embryonic development and share one of their anatomical components, the urethra. This system has a highly complex physiology deeply interconnected with the circulatory and nervous systems, as well as being capable of adapting to environmental variations; it also undergoes changes with aging and, in the case of the reproductive system, with seasonality.The stroma is an essential component in this physiological plasticity and its complexity has increased with the description in the last decade of a new cell type, the telocyte. Several studies have demonstrated the presence of telocytes in the organs of the male urogenital system and other systems; however, their exact function is not yet known. The present review addresses current knowledge about telocytes in the urogenital system in terms of their locations, interrelationships, possible functions and pathological implications. It has been found that telocytes in the urogenital system possibly have a leading role in stromal tissue organization/maintenance, in addition to participation in stem cell niches and an association with the immune system, as well as specific functions in the urogenital system, lipid synthesis in the testes, erythropoiesis in the kidneys and the micturition reflex in the bladder. There is also evidence that telocytes are involved in pathologies in the kidneys, urethra, bladder, prostate, and testes.

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“…Leydig cells are the main source of male sex hormones (Li et al, 2016). Telocytes are widespread interstitial cells observed in the testes of humans (Marini et al, 2018), rabbits (Awad and Ghanem, 2018), and Chinese soft-shelled turtles (Yang et al, 2015), but no previous study has recorded telocytes in the testes of birds.…”

Section: Introductionmentioning

confidence: 99%

Histological, histochemical, immunohistochemical and ultrastructural characterization of the testes of the dove

Mustafa

Elhanbaly

2020

Zygote

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Summary Avian testes have been used in the study of germ cell transfer, importantly for understanding the preservation and control of birds. For this purpose, we use light microscopy, electron microscopy and immunohistochemistry to understand the reproductive efficiency of dove testes. The tunica albuginea was thin and septula testes were not observed. The testicular parenchyma was formed mainly of closely packed convoluted seminiferous tubules with little interstitial area. Three types of spermatogonia were distinguished. The primary spermatocyte appeared as the largest spermatogenic cell and was identified at different stages of meiosis. Different morphological stages of the spermatid were categorized. Various cellular associations were described within the seminiferous epithelium. The cytoplasm of Sertoli cells was pale and ill defined due to its close relationship to the germinal epithelium. The spermatid attached to the luminal border of Sertoli cells and germ cells were closely associated. A single layer of myoid cells surrounded the seminiferous tubule. Testicular telocytes of doves were located in the peritubular region and near the blood vessels. Telopods appeared as long cytoplasmic processes arising from the cell body. Leydig cells were distributed singly or in small groups and cords. The intensity of androgen receptor (AR) immunostaining in the testes of the dove was established for the first time and is described in this paper.

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Localization of telocytes in rabbits testis: Histological and immunohistochemical approach

Cited by 11 publications

References 35 publications

Morphological changes of telocytes in camel efferent ductules in response to seasonal variations during the reproductive cycle

Morphological changes of telocytes in camel efferent ductules in response to seasonal variations during the reproductive cycle

Telocytes of the male urogenital system: Interrelationships, possible functions, and pathological implications

Histological, histochemical, immunohistochemical and ultrastructural characterization of the testes of the dove

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