Histological, histochemical, immunohistochemical and ultrastructural characterization of the testes of the dove

Abstract

Summary Avian testes have been used in the study of germ cell transfer, importantly for understanding the preservation and control of birds. For this purpose, we use light microscopy, electron microscopy and immunohistochemistry to understand the reproductive efficiency of dove testes. The tunica albuginea was thin and septula testes were not observed. The testicular parenchyma was formed mainly of closely packed convoluted seminiferous tubules with little interstitial area. Three types of spermatogonia were distinguished. The primary spermatocyte appeared as the largest spermatogenic cell and was identified at different stages of meiosis. Different morphological stages of the spermatid were categorized. Various cellular associations were described within the seminiferous epithelium. The cytoplasm of Sertoli cells was pale and ill defined due to its close relationship to the germinal epithelium. The spermatid attached to the luminal border of Sertoli cells and germ cells were closely associated. A single layer of myoid cells surrounded the seminiferous tubule. Testicular telocytes of doves were located in the peritubular region and near the blood vessels. Telopods appeared as long cytoplasmic processes arising from the cell body. Leydig cells were distributed singly or in small groups and cords. The intensity of androgen receptor (AR) immunostaining in the testes of the dove was established for the first time and is described in this paper.

Telocytes of the male urogenital system: Interrelationships, possible functions, and pathological implications

“…In doves, telocytes are located in the peritubular region, specifically close to blood vessels. The interstitium is greatly reduced in these testes and the myoid cells form a single cell layer (Mustafa & Elhanbaly, 2020). In the Chinese soft-shelled turtle, telocytes are connected with each other, as well as with pericytes and Leydig cells (Yang et al, 2015).…”

Morph‐anatomic and histochemical study of ileum of goose (Alopochen egyptiacus) with special references to immune cells, mucous and serous goblet cells, telocytes, and dark and light smooth muscle fibers

“…The scanning and TEM images were digitally colored using Photoshop (Adobe, CA) as described by Abdel Hakeem, Mahmoud, & Abdel‐Hafeez (2019). Many authors (Abd‐Elhafeez, Abdo, Kamal, & Soliman, 2020; Abd‐Elhafeez, Abou‐Elhamd, & Soliman, 2020; Abd‐Elhafeez, Hassan & Manal, 2020; Abd‐Elhafeez & Soliman, 2016, 2017; Abdel Hakeem, Soliman, Abd‐Elhafeez, Abdel‐Hafez, & Zaki, 2019; Abdel‐Maksoud, Abd‐Elhafeez, & Soliman, 2019, Abdel‐Maksoud, Hussein, & Attaai, 2019; Ibrahim & Abdel‐Maksoud, 2019; Mustafa & El‐Desoky, 2020; Mustafa & Elhanbaly, 2020; Soha Soliman, Hanan Hassan, & Enas, 2017; Soliman, 2019; Soliman & Abd Elhafeez, 2014; Soliman & Abd‐Elhafeez, 2016a, 2016b; Soliman, Kamal, & Abd‐Elhafeez, 2019; Soliman et al, 2020) have used this method.…”

Endocrine, Stemness, Proliferative, and Proteolytic Properties of Alarm Cells in Ruby-Red-Fin Shark (Rainbow Shark), Epalzeorhynchos frenatum (Teleostei: Cyprinidae)

“…To achieve antigen localization, we used the avidin–biotin complex technique with the reagent of the Lab Vision Ultra Vision Detection System: anti-polyvalent, horseradish peroxidase/3,3′-diaminobenzidine (DAB), ready-to-use (Thermo Fisher Scientific, Waltham, MA, USA), according to the manufacturer's instructions. The staining was done according to Abd-Elhafeez & Soliman (2017) and Mustafa & Elhanbaly, (2020a, 2020 b )…”