Localization of neuropeptide Y in human sympathetic ganglia: Correlation with met-enkephalin, tyrosine hydroxylase and acetylcholinesterase

Järvi, Riitta; Pelto‐Huikko, Markku

doi:10.1007/bf01885786

Cited by 22 publications

(7 citation statements)

References 32 publications

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“…The uneven pattern of NPY-immunoreactivity in neuronal cell bodies has also been described in studies of the central nervous system and sympathetic ganglia (Järvi and Pelto-Huikko 1990;Milner and Veznedaroglu 1992), and our observations of its association with the Golgi complex, cisterns of granular endoplasmic reticulum and large granular vesicles are consistent with previous reports (Fehér and Burnstock 1987;Gulbenkian et al 1990;Järvi and PeltoHuikko 1990;Milner and Veznedaroglu 1992). However, in addition, we noted that some multivesicular bodies were also intensely NPY-immunoreactive.…”

Section: Discussionsupporting

confidence: 93%

Neuropeptide Y-immunoreactive intracardiac neurones, granule-containing cells and nerves associated with ganglia and blood vessels in rat and guinea-pig heart

Sosunov

Hassall

Loesch

et al. 1997

Cell and Tissue Research

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Intrinsic neuropeptide Y-containing neurones in rat and guinea-pig hearts were studied at the ultrastructural level by the pre-embedding peroxidase-antiperoxidase immunocytochemical technique. Intracardiac neuronal cell bodies were often weakly or moderately immunostained, and the labelling was usually pronounced in the Golgi complex, multivesicular bodies, some cisterns of granular endoplasmic reticulum and large granular vesicles. Neuropeptide Y-immunoreactive nerve fibres were also observed in association with intracardiac neurones. A subpopulation of neuropeptide Y-immunoreactive granule-containing cells in the rat heart are described for the first time and were very heavily labelled; other granule-containing cells were non-immunoreactive, but were contacted by neuropeptide Y-containing nerves. Preterminal regions of nerve fibres that were located in nerve bundles were only weakly neuropeptide Y-immunoreactive, in contrast to the heavy labelling observed in varicosities that contained many synaptic vesicles. Many neuropeptide Y-immunoreactive nerve fibres were associated with the coronary vasculature and were particularly prominent in the walls of small arteries and arterioles where labelled nerve varicosities were present close to the smooth muscle cells. Immunoreactive nerves were also seen in the myocardium, usually near to capillaries. In axonal varicosities, the central core of large granular vesicles was immunolabelled, and electron-dense immunoreactive material outlined the membranes of small and large clear vesicles. The significance of neuropeptide Y-immunoreactive intracardiac neurones and granule-containing cells and the origin of associated labelled nerve fibres in the heart are discussed.

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Section: Discussionsupporting

confidence: 93%

Neuropeptide Y-immunoreactive intracardiac neurones, granule-containing cells and nerves associated with ganglia and blood vessels in rat and guinea-pig heart

Sosunov

Hassall

Loesch

et al. 1997

Cell and Tissue Research

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“…5A,B; Table 2, group 2). Similar NPY IR and TH IR colocalization patterns have been identified in human lumbar sympathetic ganglia (Järvi and Pelto-Huikko 1990). This population of small neurons (Table 2, groups 1c, 2) located around the superior vena cava differed from the larger ganglionic neurons, which displayed dense NPY IR primarily in the perinuclear region (Figs.…”

Section: Discussionsupporting

confidence: 70%

Distribution of intrinsic cardiac neurons in whole-mount guinea pig atria identified by multiple neurochemical coding

Horackova

Armour

Byczko

1999

Cell and Tissue Research

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Functional data indicate that neurons in distinct regions of the heart exert preferential regional cardiac control. To date the regional distribution of specific types of neurons within the intrinsic cardiac nervous system remains unknown, as does their associations with distinct neurotransmitter and/or neuromodulatory profiles. This study was designed to ascertain: (1) the distribution of different classes of neurons within the intrinsic cardiac nervous system as determined by microscopic analysis; (2) the neurochemical profiles of neurons in differing atrial loci; (3) which neurochemicals are co-localized within specific populations of intrinsic cardiac neurons; and (4) the distribution of specific sub-populations of neurons expressing specific immunoreactivities. Taking advantage of confocal laser scanning microscopy and distinct immunoreactive fluorescent markers in various double-label combinations, several sub-populations of intrinsic cardiac neurons were identified. Of all identified neurons, 85-90% were located in ganglia (ganglionic neurons), the rest being isolated (individual neurons). The two general neuronal markers protein gene product 9.5 (PGP 9.5) and microtubule-associated protein (MAP-2) were associated with neurons clustered primarily in the interatrial septum and around the origins of the two vena cavae. Ganglia (group 1) contained three sub-populations of neurons: approx. 80% of ganglionic neurons were large (15-40 microm diameters; group 1a) and approx. 20% had smaller diameters (less than 15 microm; group 1b). All of these neurons were PGP-immunoreactive, exhibiting choline acetyltransferase (ChAT) immunoreactivity (IR), tyrosine hydroxylase (TH) IR, neuropeptide Y (NPY) IR, vasoactive peptide (VIP) IR and substance P (SP) IR. The remaining 5% of ganglionic neurons were small (group 1c; less than 20 microm). These displayed TH immunoreactivity but not MAP, PGP, CHAT, NPY or SP immunoreactivity. Ten to fifteen percent of all neurons loosely distributed outside of ganglia were small (10-25 microm) and located primarily around the origin of the superior vena cava. They displayed immunoreactivity to TH, ChAT, VIP, NPY and SP, but not to MAP-2 or PGP 9.5. These data provide anatomical and immunohistochemical evidence for specific localization of differing populations of intrinsic cardiac neurons with respect to their size, ganglionic distributions and capacity to express multiple neurotransmitters. Although the functional importance of such a regional distribution of differing populations of intrinsic cardiac neurons remains unknown, these anatomical data support the thesis that unique clustering of specific populations of neurons within this nervous system represents the anatomical substrate for complex local cardiac regulatory phenomena occurring at the level of the target organ.

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“…A large number of sympathetic spinal neurons (1000-2000/ganglion) terminate on the ganglia which control the head or thoracic organs and a relatively small number (100-400/ganglion) terminate on ganglia controlling the gut, kidney, and pelvic organs in the rat (Strack et al, 1988). The great majority of paravertebral ganglion neurons are adrenergic (Jarvi and Pelto-Huikko, 1990;Tajti et al, 1999). They also contain acetylcholine, calcitonin gene-related peptide (CGRP), dopamine-beta-hydroxylase, galanin, neuropeptide Y, nitric oxide, somatostatin, tyrosine hydroxylase, and vasoactive intestinal polypeptide (VIP) (Jarvi et al, 1987;Schmitt et al, 1988, Lindh et al, 1989Jarvi and Pelto-Huikko, 1990;Baffi et al, 1992;Dun et al, 1992Dun et al, , 1993Benarroch, 1994;Vizzard et al, 1994;Tajti et al, 1999;Zhou and Ling, 1999).…”

Section: Spinal Nervesmentioning

confidence: 99%

The Spinal Nerves

Kayalıoğlu¹

2009

The Spinal Cord

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Localization of neuropeptide Y in human sympathetic ganglia: Correlation with met-enkephalin, tyrosine hydroxylase and acetylcholinesterase

Cited by 22 publications

References 32 publications

Neuropeptide Y-immunoreactive intracardiac neurones, granule-containing cells and nerves associated with ganglia and blood vessels in rat and guinea-pig heart

Neuropeptide Y-immunoreactive intracardiac neurones, granule-containing cells and nerves associated with ganglia and blood vessels in rat and guinea-pig heart

Distribution of intrinsic cardiac neurons in whole-mount guinea pig atria identified by multiple neurochemical coding

The Spinal Nerves

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