Localization of carbonic anhydrase in identified glial cells of the leech central nervous system: application of histochemical and immunocytochemical methods.

Riehl, B.; Schlue, Wolf-Rüdiger

doi:10.1177/38.8.2114440

Cited by 8 publications

(3 citation statements)

References 28 publications

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“…The ganglia and connectives show an identical organization, and they contain neuranes and glial cells that can be identified and distinguished according to their location and electrical properties (Schlue et ale 1980;Riehl and Schlue 1990). …”

Section: Te Leech Prep~rationmentioning

confidence: 99%

The regulation of pH in the central nervous system

Schlue

Dörner²

1992

Can. J. Physiol. Pharmacol.

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The pHi regulation from intracellular acidosis in the central nervous system appears to be mediated by mechanisms driven by the large inwardly directed Na+ gradient. The involvement of these mechanisms in pHi regulation of neurones and glial cells has been investigated in the leech central nervous system using ion-selective microelectrodes. For recovery from acidification, there appear to be three separate mechanisms: Na+/H+ exchange, Na(+)-dependent Cl-/HCO3- exchange, and Na+-HCO3- cotransport. All three mechanisms have a profound effect on the maintenance of pHi homeostasis in glial cells; whereas in leech neurones, as in other neuronal cells studied previously, the predominant mechanisms are Na+/H+ and Na(+)-dependent Cl-/HCO3- exchange. In addition to acid extrusion mechanisms we also found evidence for Na(+)-independent Cl-/HCO3- exchange. At alkaline pHi this exchanger may mediate some of the pHi recovery from intracellular alkalinization.

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Section: Te Leech Prep~rationmentioning

confidence: 99%

The regulation of pH in the central nervous system

Schlue

Dörner²

1992

Can. J. Physiol. Pharmacol.

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“…Therefore, CA is an essential factor in acid-base balance and a transport mechanism for CO, and CA was localized in frozen sections of the leech CNS. 36 Hansson's cobalt precipitation method and the use of DNSA to build a fluorescent enzyme-substrate complex showed that glial cells are the sites of CA activity in the leech. Neuropile and connective glial cells surrounding the axons had strong CA activity, whereas packet glial cells, which surround neuron cell bodies, and neurons themselves remained unstained.…”

Section: Localization Of Carbonic Anhydrase By Andplication Of Histochementioning

confidence: 99%

Glial H⁺ Transport and Control of pH

Schlue

Dörner

Rempe

et al. 1991

Annals of the New York Academy of Sciences

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“…The ganglia of the leech nervous system show an identical organization, and they contain glial cells that can be identified and distinguished according to their location and electrical properties (Riehl and Schlue, 1990; . Six packet glial cells in each ganglion surround the neuron cell bodies, and two neuropile glial (NG) cells enclose the neuron processes and synaptic regions.…”

Section: Introductionmentioning

confidence: 99%

Single ion channel currents in neuropile glial cells of the leech central nervous system

Müller

Hanke

Schlue

1993

Glia

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The patch-clamp technique was used to investigate the activity of single ion channels in neuropile glial (NG) cells in the central nervous system (CNS) of the medicinal leech, Hirudo medicinalis. We found evidence for two distinct Cl- channels that could be distinguished by their basic electrical properties and their responses to different inhibitors on single ion channel currents. In the inside-out configuration in symmetrical Cl- solutions, these channels showed current-voltage relationships with slight outward rectification, mean conductances of 70 and 80 pS, and reversal potentials near 0 mV. Significant permeability to Na+, K+, or SO4(2-) could not be detected. The open-state probability of the 70 pS Cl- channel increased with membrane depolarization, whereas the open-state probability of the 80 pS Cl- channel was voltage-independent. The application of the stilbene derivative DIDS (100 microM) to the cytoplasmic side of the glial cell membrane blocked both Cl- channels. The activity of the 70 pS channel was blocked irreversibly by DIDS, whereas the activity of the 80 pS channel reappeared after wash-out of DIDS. Both channels were blocked reversibly by 1 mM Zn2+. K+ channels could only be observed occasionally in the soma membrane of the NG cells. We have characterized a 60 pS K+ channel with a high selectivity for K+ over Na+. The low density of K+ channels in the soma membrane may indicate a non-uniform distribution of this channel type in NG cells.

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Localization of carbonic anhydrase in identified glial cells of the leech central nervous system: application of histochemical and immunocytochemical methods.

Cited by 8 publications

References 28 publications

The regulation of pH in the central nervous system

The regulation of pH in the central nervous system

Glial H⁺ Transport and Control of pH

Single ion channel currents in neuropile glial cells of the leech central nervous system

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Localization of carbonic anhydrase in identified glial cells of the leech central nervous system: application of histochemical and immunocytochemical methods.

Cited by 8 publications

References 28 publications

The regulation of pH in the central nervous system

The regulation of pH in the central nervous system

Glial H+ Transport and Control of pH

Single ion channel currents in neuropile glial cells of the leech central nervous system

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Product

Resources

About

Glial H⁺ Transport and Control of pH