1993
DOI: 10.1002/glia.440090404
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Single ion channel currents in neuropile glial cells of the leech central nervous system

Abstract: The patch-clamp technique was used to investigate the activity of single ion channels in neuropile glial (NG) cells in the central nervous system (CNS) of the medicinal leech, Hirudo medicinalis. We found evidence for two distinct Cl- channels that could be distinguished by their basic electrical properties and their responses to different inhibitors on single ion channel currents. In the inside-out configuration in symmetrical Cl- solutions, these channels showed current-voltage relationships with slight outw… Show more

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Cited by 5 publications
(2 citation statements)
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“…The bilayer set‐up consists of two chambers connected by a 150 μm aperture through a Delrin cup. Painted planar bilayers were formed in the aperture from a 10 mg/ml n ‐decane solution of PE:PC (7:3, w/w) as described [13,14]. The cup was placed in a black Delrin chamber and filled with 1 ml bilayer buffer (150 mM NaCl, 8 mM HEPES, pH 7.5).…”
Section: Methodsmentioning
confidence: 99%
“…The bilayer set‐up consists of two chambers connected by a 150 μm aperture through a Delrin cup. Painted planar bilayers were formed in the aperture from a 10 mg/ml n ‐decane solution of PE:PC (7:3, w/w) as described [13,14]. The cup was placed in a black Delrin chamber and filled with 1 ml bilayer buffer (150 mM NaCl, 8 mM HEPES, pH 7.5).…”
Section: Methodsmentioning
confidence: 99%
“…Their dissection as well as the isolation of the ganglia was done as described by Schlue and Deitmer (1980). After isolation of the ganglia, the neuropile glial cells were exposed by removing the ventral part of the outer ganglion capsule and the ventral neuronal somata (Mü ller et al, 1993).…”
Section: Materials and Methods Preparationmentioning
confidence: 99%