Localisation des gènes MPI, PKM2, NP sur le chromosome 3 du porc (Sus scrofa L.) et analyse cytogénétique d'une lignée de hamster chinois issue de la DON (wg3h)

Echard, G.; Gellin, Joël; Gillois, M.; Banizette, Chantal de

doi:10.1186/1297-9686-16-3-261

Cited by 24 publications

(12 citation statements)

References 8 publications

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“…Briefly, normal diploid female chicken fibroblasts irradiated at 6000 rads by gamma rays from a Cesium-137 source were fused to the hypoxanthine guanine phosphoribosyl transferase (HPRT)-deficient hamster cell line, Wg3hCl2 [13]. The hybrid cells were selected on HAT (hypoxanthine-aminopterin-thymidine) media, tested for marker retention and subjected to large-scale culture for DNA extraction.…”

Section: Radiation Hybrid Panelmentioning

confidence: 99%

A gene-based radiation hybrid map of chicken microchromosome 14: Comparison to human and alignment to the assembled chicken sequence

et al. 2005

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-We present a gene-based RH map of the chicken microchromosome GGA14, known to have synteny conservations with human chromosomal regions HSA16p13.3 and HSA17p11.2. Microsatellite markers from the genetic map were used to check the validity of the RH map and additional markers were developed from chicken EST data to yield comparative mapping data. A high rate of intra-chromosomal rearrangements was detected by comparison to the assembled human sequence. Finally, the alignment of the RH map to the assembled chicken sequence showed a small number of discordances, most of which involved the same region of the chromosome spanning between 40.5 and 75.9 cR 6000 on the RH map.chicken / comparative mapping / radiation hybrids / microchromosome 14 / intrachromosomal rearrangement

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Section: Radiation Hybrid Panelmentioning

confidence: 99%

A gene-based radiation hybrid map of chicken microchromosome 14: Comparison to human and alignment to the assembled chicken sequence

et al. 2005

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“…They were mixed to the same number of recipient cells of the hypoxanthine guanine phosphoribosyl transferase (HPRT)-deficient hamster cell line, Wg3hCl 2 [7]. Fusion partners were first pelleted and then suspended in 1 mL of polyethyleneglycol (Roche Diagnostics GmbH).…”

Section: Generation Of Radiation Hybridsmentioning

confidence: 99%

ChickRH6: a chicken whole-genome radiation hybrid panel

et al. 2002

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-As a first step towards the development of radiation hybrid maps, we have produced a radiation hybrid panel in the chicken by fusing female embryonic diploid fibroblasts irradiated at 6 000 rads with HPRT-deficient hamster Wg3hCl 2 cells. Due to the low retention frequency of the chicken fragments, a high number of clones was produced from which the best ones were selected. Thus, 452 fusion clones were tested for retention frequencies with a panel of 46 markers. Based on these results, 103 clones with a mean marker retention of 23.8% were selected for large scale culture to produce DNA in sufficient quantities for the genotyping of numerous markers. Retention frequency was tested again with the same 46 markers and the 90 best clones, with a final mean retention frequency of 21.9%, were selected for the final panel. This panel will be a valuable resource for fine mapping of markers and genes in the chicken, and will also help in building BAC contigs. chicken / radiation hybrid / mapping

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“…Parental cells were blood lymphocytes from two Egyptian river buffaloes, and cells of the HPRT-(hypoxanthine phosphoribosyltransferase-negative) Chinese hamster cell line wg3h C 1 2 (Echard et al, 1984). Hybrid cells were produced by polyethylene glycol (PEG)-mediated fusion, followed by hypoxanthine-aminopterinethymidine (HAT) selection.…”

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confidence: 99%

Gene mapping in the river buffalo (Bubalus bubalis L)

Hondt¹,

Bosma²,

Bieman³

et al. 1991

Genet. Sel. Evol.

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Localisation des gènes MPI, PKM2, NP sur le chromosome 3 du porc (Sus scrofa L.) et analyse cytogénétique d'une lignée de hamster chinois issue de la DON (wg3h)

Abstract: RésuméL'analyse par électrophorèse de clones cellulaires hybrides interspécifiques porc-hamster chinois nous a permis de démontrer que les gènes MPI, PKM2, NP sont synténiques chez le porc (G ELUN et al., 1981). Le

Cited by 24 publications

References 8 publications

A gene-based radiation hybrid map of chicken microchromosome 14: Comparison to human and alignment to the assembled chicken sequence

A gene-based radiation hybrid map of chicken microchromosome 14: Comparison to human and alignment to the assembled chicken sequence

ChickRH6: a chicken whole-genome radiation hybrid panel

Gene mapping in the river buffalo (Bubalus bubalis L)

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