Gene mapping in the river buffalo (Bubalus bubalis L)

Comparative FISH-mapping of the prion protein gene (PRNP) on cattle, river buffalo, sheep and goat chromosomes

“…This is the first molecular marker assignment to CHI13 and BBU14 and allowed us to indirectly assign the bovine syntenic group U11 (expressed genes only) (ADA, ARVP, HCK, IL2R, ITPA, OXT, SOD1L1, VIM; Eggen and Fries, 1995) to these chromosomes. Our data in river buffalo agree with those recently reported by De Hondt et al (1997) who assigned two markers of bovine U11 (OXT and PRNP) to the river buffalo genome using the somatic cell hybrid technique. FISH-mapping of PRNP to BTA13q17 agrees with the ZOO-FISH mapping performed in cattle using specific human chromosome painting probes.…”

Assignment of PCR markers to river buffalo chromosomes

“…The present study confirms syntenic conservation between cattle and buffalo as previously demonstrated [6,7,11,32]. Chromosomal conservation between cattle and buffalo has also been demonstrated, based on the banding homoeology between cattle and river buffalo chromosomes [22].…”

“…Forty-seven somatic hybrid cell lines were developed as described previously [6] from fusion between buffalo lymphocytes and the Chinese hamster cell line wg3h [9].…”

“…Therefore, inferences can be made concerning the expected location of markers in one species from information available in another species. Several genes previously assigned to cattle syntenic groups and/or chromosomes have been assigned to buffalo chromosomes [6,7,10,11,18,19,23,24,28].…”

ZOO-FISH and R-banding reveal extensive conservation of human chromosome regions in euchromatic regions of river buffalo chromosomes

“…ZOO-FISH studies using human chromosome paints have been reported in such domestic animals as cattle (Hayes, 1995;Solinas-Toldo et al, 1995;Chowdhary et al, 1996), pig Rettenberger et al, 1995), horse (Raudsepp et al, 1996), and cat (O'Brien et al, 1997), but they are still lacking in other economically important domestic species, such as the river buffalo, where only a few loci have been physically mapped using both somatic cell hybrid (El Nahas et al, 1996;De Hondt et al, 1997) and in situ hybridization techniques (Hassaname et al, 1993(Hassaname et al, , 1994Iannuzzi et al, 1997a-d). Furthermore, the difficulty in clearly identifying small acrocentric chromosomes, especially when banding techniques are applied with contracted chromosome preparations, lends greater importance to the ZOO-FISH analysis in river buffalo (Bubalus bubalis, BBU) chromosomes owing to the presence of marker chromosome pairs involving small, disputed cattle (Bos taurus, BTA) chromosomes in the standardized nomenclatures (Iannuzzi, 1996).…”