Local delivery of bone morphogenetic protein-2 from near infrared-responsive hydrogels for bone tissue regeneration

Sánchez-Casanova, Silvia; Martín-Saavedra, Francisco M.; Escudero-Duch, Clara; Falguera-Uceda, María Isabel; Prieto, Martín; Arruebo, Manuel; Acebo, Paloma; Fabiilli, Mario L.; Franceschi, Renny T.; Vilaboa, Nuria

doi:10.1016/j.biomaterials.2020.119909

Cited by 49 publications

(53 citation statements)

References 66 publications

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“…In order to apply Pi more effectively, the transport and sustained-release of Pi are the key problems to be solved in its clinical application. Multiple delivery systems are under study [ 28 – 31 ], and those findings bring hope to us. Polyphosphate, a linear polymer of orthophosphates which can be polymerized to length of hundreds of phosphates, can be used as an effective source of Pi [ 32 ].…”

Section: Discussionmentioning

confidence: 99%

Effect of inorganic phosphate on migration and osteogenic differentiation of bone marrow mesenchymal stem cells

et al. 2021

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Background Phosphate is the major ingredient of bone tissue, and is also an important component of commercial bone substitute materials, bone scaffolds, and implant surface coatings. With the dissolution of the bone substitute materials and the degradation by cells, local ion concentrations will change and affect bone tissue reconstruction. Bone marrow -derived mesenchymal stem cells (BM-MSCs) are main autologous cells to repair injured bone. When bone injure occurs, BM-MSCs migrate to the damaged area, differentiate into osteoblasts, and secrete bioactive factors to promote bone tissue repaired. This study aimed to investigate the effect of inorganic phosphate (Pi) at a series of concentration on migration and osteogenic differentiation of human bone marrow -derived mesenchymal stem cells(hBM-MSCs). Methods The culture of hBM-MSCs in mediums with different concentration of Pi from 2 mM to 10 mM were performed. HBM-MSCs migration were examined with transwell assays. HBM-MSCs proliferation were evaluated by cell counting kit-8 colorimetric method. Osteogenic genes expression were analyzed by real-time reverse transcriptase polymerase chain reaction. Mineralized nodules formation were demonstrated by Alizarin red staining. Result 4–10 mM Pi could effectively promote the migration of hBM-MSCs at 12 h and 18 h. There was no significant difference in the migration number of hBM-MSCs in Pi culture mediums at a concentration of 6, 8, and10mM. 2–10 mM Pi could promote the proliferation of hBM-MSCs to varying degrees in the observation period, while 4–10 mM Pi could promote the osteogenic differentiation and mineralization of hBM-MSCs. Conclusion The findings in our study showed 4-10 mM Pi could promote the migration, osteogenic differentiation, and mineralization of hBM-MSCs.

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Section: Discussionmentioning

confidence: 99%

Effect of inorganic phosphate on migration and osteogenic differentiation of bone marrow mesenchymal stem cells

et al. 2021

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“…Controlled release could be triggered under NIR light irradiation, promoting the healing process during bone tissue repair. Moreover, another heat-activated and dimerizer-dependent transgene expression system was designed based on an NIR light-responsive hydrogel to achieve spatiotemporal control of bone morphogenetic protein-2 (BMP-2) 21 . The photothermal effect of hollow gold nanoparticles in hydrogels induced mild hyperthermia, which stimulated the cell constructs to express BMP-2 in the bone defect.…”

Section: Discussionmentioning

confidence: 99%

“…The precise regulation of photothermal effect during the NIR light irradiation treatment is essential to promote the efficacy of phototherapies and minimize the damage. Considering the different therapeutic goals in the treatment of bone-related diseases, the parameters of NIR light irradiation to generate targeted hyperthermia within a reasonable temperature range should be carefully selected 21 . Generally speaking, local hyperthermia over 50 °C exerts highly effective phototherapeutic effects on pathological tissues, which is favorable for the treatment of bone cancers, infections, and inflammations.…”

Section: Current Challenges and Perspectivesmentioning

confidence: 99%

“…At the same time, several studies have demonstrated that NIR light triggered mild localized heat, and the photoelectronic microenvironment could accelerate bone tissue regeneration 19 , 20 . Moreover, NIR light-responsive drug delivery systems have not only been used for targeted cancer therapies, but also for the delivery of growth factors for bone tissue regeneration 21 , 22 . These latest studies have revealed that NIR light‑assisted phototherapy is likely to realize more precise and less invasive therapeutic solutions not only for bone cancers, but also for implant infections, osteochondral inflammation, and bone tissue regeneration.…”

Section: Introductionmentioning

confidence: 99%

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NIR light-assisted phototherapies for bone-related diseases and bone tissue regeneration: A systematic review

Wan¹,

Zhang²,

Lv³

et al. 2020

Theranostics

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Recently, the rapid development of biomaterials has induced great interest in the precisely targeted treatment of bone-related diseases, including bone cancers, infections, and inflammation. Realizing noninvasive therapeutic effects, as well as improving bone tissue regeneration, is essential for the success of bone‑related disease therapies. In recent years, researchers have focused on the development of stimuli-responsive strategies to treat bone-related diseases and to realize bone regeneration. Among the various external stimuli for targeted therapy, near infrared (NIR) light has attracted considerable interests due to its high tissue penetration capacity, minimal damage toward normal tissues, and easy remote control properties. The main objective of this systematic review was to reveal the current applications of NIR light-assisted phototherapy for bone-related disease treatment and bone tissue regeneration. Database collection was completed by June 1, 2020, and a total of 81 relevant studies were finally included. We outlined the various therapeutic applications of photothermal, photodynamic and photobiomodulation effects under NIR light irradiation for bone‑related disease treatment and bone regeneration, based on the retrieved literatures. In addition, the advantages and promising applications of NIR light-responsive drug delivery systems for spatiotemporal-controlled therapy were summarized. These findings have revealed that NIR light-assisted phototherapy plays an important role in bone-related disease treatment and bone tissue regeneration, with significant promise for further biomedical and clinical applications.

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“…Hydrogels have highly hydrated and interconnected structures, which can be formed in situ after injection. Besides, hydrogels with simple preparation, low toxicity, can also be applied to the responsiveness of the environment [64,65]. Bioactive glass can induce bone matrix formation and enhance mineralization [66].…”

Section: Recombinationmentioning

confidence: 99%

Advances in the application of gold nanoparticles in bone tissue engineering

Xia

et al. 2020

J Biol Eng

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The materials used in bone tissue engineering (BTE) have been advancing with each passing day. With the continuous development of nanomedicine, gold nanoparticles (GNPs), which are easy to be synthesized and functionalized, have attracted increasing attention. Recent years have witnessed this amazing material, i.e., GNPs characterized with large surface area to volume ratio, biocompatibility, medical imaging property, hypotoxicity, translocation into the cells, high reactivity, and other properties, perform distinct functions in BTE. However, the low stability of GNPs in the biotic environment makes them in the requirements of modification or recombination before being used. After being combined with the advantages of other materials, the structures of GNPs have exhibited great potential in stem cells, scaffolds, delivery systems, medical imaging, and other aspects. This review will focus on the advances in the application of GNPs after modification or recombination with other materials to BTE.

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Local delivery of bone morphogenetic protein-2 from near infrared-responsive hydrogels for bone tissue regeneration

Cited by 49 publications

References 66 publications

Effect of inorganic phosphate on migration and osteogenic differentiation of bone marrow mesenchymal stem cells

Effect of inorganic phosphate on migration and osteogenic differentiation of bone marrow mesenchymal stem cells

NIR light-assisted phototherapies for bone-related diseases and bone tissue regeneration: A systematic review

Advances in the application of gold nanoparticles in bone tissue engineering

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