Effect of inorganic phosphate on migration and osteogenic differentiation of bone marrow mesenchymal stem cells

Abstract

Background Phosphate is the major ingredient of bone tissue, and is also an important component of commercial bone substitute materials, bone scaffolds, and implant surface coatings. With the dissolution of the bone substitute materials and the degradation by cells, local ion concentrations will change and affect bone tissue reconstruction. Bone marrow -derived mesenchymal stem cells (BM-MSCs) are main autologous cells to repair injured bone. When bone injure occurs, BM-MSCs migrate to the damaged area, differentiate into osteoblasts, and secrete bioactive factors to promote bone tissue repaired. This study aimed to investigate the effect of inorganic phosphate (Pi) at a series of concentration on migration and osteogenic differentiation of human bone marrow -derived mesenchymal stem cells(hBM-MSCs). Methods The culture of hBM-MSCs in mediums with different concentration of Pi from 2 mM to 10 mM were performed. HBM-MSCs migration were examined with transwell assays. HBM-MSCs proliferation were evaluated by cell counting kit-8 colorimetric method. Osteogenic genes expression were analyzed by real-time reverse transcriptase polymerase chain reaction. Mineralized nodules formation were demonstrated by Alizarin red staining. Result 4–10 mM Pi could effectively promote the migration of hBM-MSCs at 12 h and 18 h. There was no significant difference in the migration number of hBM-MSCs in Pi culture mediums at a concentration of 6, 8, and10mM. 2–10 mM Pi could promote the proliferation of hBM-MSCs to varying degrees in the observation period, while 4–10 mM Pi could promote the osteogenic differentiation and mineralization of hBM-MSCs. Conclusion The findings in our study showed 4-10 mM Pi could promote the migration, osteogenic differentiation, and mineralization of hBM-MSCs.

Cytocompatibility and Bioactive Ion Release Profiles of Phosphoserine Bone Adhesive: Bridge from In Vitro to In Vivo

“…The authors were unable to find any studies describing the effect of silicon ions on inflammatory or bone resorbing cell types, or chemotaxis/cell recruitment in response to silicon gradients. Phosphorus ions stimulate the recruitment/chemotaxis (4–10 mM) [ 72 ] and osteogenic differentiation of osteoblasts and MSCs (0.5–10 mM) [ 72 , 73 , 74 ]. While it is clear that phosphate eluting ceramics can directly affect inflammation and angiogenesis [ 57 ], the authors were unable to find any studies describing the direct effects of phosphate ions on the recruitment or differentiation of inflammatory, angiogenic, or resorbing cell types.…”

Injectable, High Specific Surface Area Cryogel Microscaffolds Integrated with Osteoinductive Bioceramic Fibers for Enhanced Bone Regeneration

“…HA nanowires have the same stoichiometric composition with native bone mineral. As compared to the GM microspheres, therefore it is reasonable to find that the GMH microspheres can promote osteogenic differentiation of BMSCs in vitro , and enhance bone regeneration in vivo , because the composite microspheres can release bioactive Ca and P ions. , The released Ca and P ions from the GMH are only several ppm per day due to the limited solubility of crystalline HA, while it is reported that Ca and P ions at a concentration of ∼40 ppm may promote osteogenesis at a significant level. − As an alternate for HA, the CS-type bioceramic has attracted great attention for its fast degradation rate and the promotion of osseointegration through the released Ca and Si ions. , Ion-exchange interactions during mineralization can accelerate apatite deposition kinetics. Accordingly, in this study, the pore walls of the GMC cryogel microspheres are covered with rich apatite-like minerals after being immersed in 1.5SBF for 7 days, while the GMH microspheres show much less mineral deposition.…”

Strontium-substituted phosphate-based glass exhibits improved and optimized bioactive in vitro and in vivo responses

“…P, Ca, and Sr dissolution in uenced the cellular activity by differentiating the pre-osteoblast. P ion in uences the osteoblastic cells of mineralized tissue; it plays a critical role in the formation of hard tissue [33]. Ca ions have been shown to enhance the viability, proliferation, and differentiation of osteoblastic cells [34].…”