Local Anesthetics Depress the Calcium Current of Rat Sensory Neurons in Culture

Sugiyama, Kanako; Muteki, Takesuke

doi:10.1097/00000542-199406000-00025

Cited by 94 publications

(48 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Tetracaine influences Ca '2+ channel function in rat neurones (Sugiyama and Muteki, 1994), and in frog and arthropod muscle (Garcia et al, 1991;Scheuer and Gilly, 1986), besides the q~ charge movements linked to DHPR function in skeletal muscle (Huang, 1990). Nevertheless, it provides a consistent empirical charge separation testable against a wide range of independent criteria.…”

Section: Discussionmentioning

confidence: 99%

Kinetic isoforms of intramembrane charge in intact amphibian striated muscle.

Huang

1996

The Journal of General Physiology

View full text Add to dashboard Cite

The effects of the ryanodine receptor (RyR) antagonists ryanodine and daunorubicin on the kinetic and steady-state properties of intramembrane charge were investigated in intact voltage-clamped frog skeletal muscle fibers under conditions that minimized timedependent ionic currents. A hypothesis that RyR gating is allosterically coupled to configurational changes in dihydropyridine receptors (DHPRs) would predict that such interactions are reciprocal and that RyR modification should influence intramembrane charge. Both agents indeed modified the time course of charging transients at 100-200-p.M concentrations. They independently abolished the delayed charging phases shown by q~ currents, even in fibers held at fully polarized, -90-mV holding potentials; such waveforms are especially prominent in extracellular solutions containing gluconate. Charge movements consistently became exponential decays to stable baselines in the absence of intervening inward or other time-dependent currents. The steady-state charge transfers nevertheless remained equal through the ON and the OFF parts of test voltage steps. The charge-voltage function, Q(VT), shifted by ~+ 10 mV, particularly through those test potentials at which delayed q~ currents normally took place but retained steepness factors (k ~ 8.0 to 10.6 mV) that indicated persistent, steeply voltage-dependent q~ contributions. Furthermore, both RyR antagonists preserved the total charge, and its variation with holding potential, Qma~(VH), which also retained similarly high voltage sensitivities (k ~ 7.0 to 9.0 mV). RyR antagonists also preserved the separate identities of qv and qa species, whether defined by their steady-state voltage dependence or inactivation or pharmacological properties. Thus, tetracaine (2 mM) reduced the available steady-state charge movement and gave shallow Q(VT) (k --~ 14 to 16 mV) and Qma~(VH) (k 14 to 17 mV) curves characteristic of q~ charge. These features persisted with exposure to test agent. Finally, q~ charge movements showed steep voltage dependences with both activation (k ~ 4.0 to 6.5 mV) and inactivation characteristics (k ~ 4.3 to 6.6 mV) distinct from those shown by the remaining q~ charge, whether isolated through differential tetracaine sensitivities, or the full approximation of charge-voltage data to the sum of two Boltzmann distributions. RyR modification thus specifically alters qx kinetics while preserving the separate identities of steady-state qf~ and q~ charge. These findings permit a mechanism by which transverse tubular voltage provides the primary driving force for configurafional changes in DHPRs, which might produce q~ charge movement. However, they attribute its kinetic complexities to the reciprocal allosteric coupling by which DHPR voltage sensors and RyR-Ca 2 § release channels might interact even though these receptors reside in electrically distinct membranes. RyR modification then would still permit tubular voltage change to drive net q~ charge transfer but would transform its complex waveforms into s...

show abstract

Section: Discussionmentioning

confidence: 99%

Kinetic isoforms of intramembrane charge in intact amphibian striated muscle.

Huang

1996

The Journal of General Physiology

View full text Add to dashboard Cite

show abstract

“…In the present study, we found that local anesthetics inhibit both nerve mediated contraction induced by EFS (0.8 msec) and nonnerve mediated detrusor contraction induced by KCl, carbachol (CCh), EFS (100 msec), or Ca 2þ -independent SuTC in the human detrusor muscle. These results suggest that local anesthetics may also block L-type Ca 2þ channels [Ahn and Karaki, 1988;Sugiyama and Muteki, 1994], and that their action mechanisms may be more complex than previously believed.…”

Section: Discussionmentioning

confidence: 71%

Effects of local anesthetics on human bladder contractility

Oh¹,

Paick²,

Lim³

et al. 2005

Neurourology and Urodynamics

View full text Add to dashboard Cite

show abstract

“…Higher dose lidocaine blocks voltage-gated calcium channels, other potassium channels, and NMDA receptors [145,149,150]. Lidocaine dosages needed for voltage-gated sodium channel blockade range from 60 to 200 μM, whereas voltage-gated calcium channel blockade occurs at higher doses in the 1-10 mM range [6, [151][152][153]. A number of different sodium channel isoforms exist with distinct tissue distribution and possibly distinct physiological functions.…”

Section: The Rationale For Ivlt In the Management Of Painmentioning

confidence: 99%

A Review of Intravenous Lidocaine Infusion Therapy for Paediatric Acute and Chronic Pain Management

Lauder¹

2017

Pain Relief - From Analgesics to Alternative Therapies

View full text Add to dashboard Cite

Pediatric acute and chronic pain experiences involve the interaction of physiological, psychological, behavioural, developmental, pharmacological and situational factors. In the acute perioperative pain setting preventative multimodal analgesia is required to provide comfort and minimise the potential for "wind-up" and central sensitisation. When pain is recurrent, ongoing or chronic some children embark on a downward spiral of decreased physical, psychological and social functioning. The multidisciplinary team management approach is a well-established standard of care for children with complex chronic pain. Intravenous lidocaine has peripheral and central mediated analgesic, anti-inflammatory and anti-hyperalgesic properties. Intravenous lidocaine infusion therapy (IVLT) has been shown to be effective in the management of acute and chronic pain in adults. This chapter will present the rational for IVLT in pediatric pain management with emphasis on preventative multimodal therapy in acute pain and the multidisciplinary treatment approach in chronic pain. Large multi-centre randomised controlled trials are required to provide the evidence-base to confirm that IVLT is indeed an effective and safe treatment option in acute preventative multimodal analgesia and as an adjunct in the multidisciplinary care of chronic pain in the pediatric population. Keywords

show abstract

Local Anesthetics Depress the Calcium Current of Rat Sensory Neurons in Culture

Cited by 94 publications

References 0 publications

Kinetic isoforms of intramembrane charge in intact amphibian striated muscle.

Kinetic isoforms of intramembrane charge in intact amphibian striated muscle.

Effects of local anesthetics on human bladder contractility

A Review of Intravenous Lidocaine Infusion Therapy for Paediatric Acute and Chronic Pain Management

Contact Info

Product

Resources

About