Loading ceftriaxone, vancomycin, and Bifidobacteria bifidum TMC3115 to neonatal mice could differently and consequently affect intestinal microbiota and immunity in adulthood

Abstract

Recent studies have demonstrated that antibiotics/or probiotics administration in early life play key roles on modulating intestinal microbiota and the alterations might cause long-lasting consequences both physiologically and immunologically. We investigated the effects of early life ceftriaxone, vancomycin and Bifidobacterium bifidum TMC3115 (TMC3115) treatment on intestinal microbiota and immunity both in neonates and adults even after termination of antibiotics exposure. We found that ceftriaxone and vancomycin, but not TMC3115, significantly altered the intestinal microbiota, serum total IgE level, and the morphology and function of the intestinal epithelium in the neonatal mice. In the adult stages, the diversity and composition of the intestinal microbiota were significantly different in the antibiotic-treated mice, and ceftriaxone-treated mice exhibited significantly higher serum total IgE and OVA-specific IgE levels. TMC3115 significantly mitigated the alteration of intestinal microbiota caused by ceftriaxone not vancomycin. Antibiotics and TMC3115 can differently modulate intestinal microbiota and SCFAs metabolism, affecting the development and function of the immunity and intestinal epithelium to different degrees in neonatal mice. Neonatal ceftriaxone-induced abnormal intestinal microbiota, immunity and epithelium could last to adulthood partly, which might be associated with the enhancement of host susceptibility to IgE-mediated allergies and related immune responses, TMC3115 may protect against the side effects of antibiotic treatment, at least partly.

Bifidobacterium bifidumTMC3115 ameliorates milk protein allergy in by affecting gut microbiota: A randomized double‐blind control trial

“…(b) The therapeutic results of Bifidobacterium bifidum TMC3115 for the infants with CMPA between intervention group and control group after 6 months. The statistical difference was significant if p < .05 (Cheng et al, 2019). However, the related clinical trials or the effects of B. bifidum TMC3115 on food allergy were seldom reported.…”

“…TMC3115 modulates intestinal microbiota and SCFAs metabolism, and affects gut microbiota and immunity. The results may be associated with the improvement of IgE-mediated allergies and related immune responses (Cheng et al, 2019). Therefore, more studies on gut microbiota profiling in CMPA are necessary to understand the function of B. bifidum TMC3115 on CMPA infants.…”

Long-Term Exposure to Ceftriaxone Sodium Induces Alteration of Gut Microbiota Accompanied by Abnormal Behaviors in Mice

“…At the phylum level, the microbiota diversity of the AB group decreased, Proteobacteria became a dominant phylum, and the abundance of Bacteroidetes, Firmicutes, Actinobacteria, and Deferribacteres decreased. This result is supported by studies that ceftriaxone could characteristically decrease the alpha-diversity of the fecal microbiota accompanied with more Proteobacteria and less Bacteroidetes (Cheng et al, 2017(Cheng et al, , 2019Miao et al, 2020). In some dysbiosis and related diseases, an increased Proteobacteria is perceived as a diagnostic characteristic since it is closely related to colon epithelial oxygenation as well as the disruption of the FIGURE 10 | Concentrations of (A) IL-6, (B) IL-10, and (C) corticosterone in serum.…”

“…gut anaerobic environment (Zhu et al, 2013;Shin et al, 2015;Miao et al, 2020). Firmicutes has become a controversial strain as some studies identified an increase in Firmicutes after ceftriaxone treatment, but others have demonstrated a declined Firmicutes in the ceftriaxone group (Cheng et al, 2017(Cheng et al, , 2019Miao et al, 2020). Evidence from clinics has suggested that patients with depression often have decreased Firmicutes (Huang et al, 2018).…”

Effect of heat-inactivated <i>Lactobacillus paracasei</i> N1115 on microbiota and gut-brain axis related molecules

“…Total DNA was extracted using TIANamo Stool DNA kits (Tiangen Biotech Co. Ltd., Beijing, China). The reaction for next-generation sequencing was conducted as described in our previous work [26]. The data from the next-generation sequencing in this study can be found in NCBI BioProject database under accession number PRJNA546058.…”