LncRNA FENDRR Servers as a Possible Marker of Essential Hypertension and Regulates Human Umbilical Vein Endothelial Cells Dysfunction via miR-423-5p/Nox4 Axis

Zhao, Xianzheng; Wang, Chen; Liu, Min; Meng, Fansen; Li, Kai

doi:10.2147/ijgm.s338147

Cited by 7 publications

(4 citation statements)

References 43 publications

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“…In this study, we identified a new inhibitor of sow GC apoptosis and FA initiation: miR-423. In other normal cell types such as bone marrow mesenchymal stem cells [ 36 ], cardiomyocyte [ 37 ], and human umbilical vein endothelial cells [ 38 ], miR-423 has been shown to function as an anti-apoptotic modulator. Thus, our study reports a potential small molecule for alleviating FA and improving female fertility.…”

Section: Discussionmentioning

confidence: 99%

miR-423 sponged by lncRNA NORHA inhibits granulosa cell apoptosis

Li,

Zhang,

Wang

et al. 2023

J Animal Sci Biotechnol

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Background Atresia and degeneration, a follicular developmental fate that reduces female fertility and is triggered by granulosa cell (GC) apoptosis, have been induced by dozens of miRNAs. Here, we report a miRNA, miR-423, that inhibits the initiation of follicular atresia (FA), and early apoptosis of GCs. Results We showed that miR-423 was down-regulated during sow FA, and its levels in follicles were negatively correlated with the GC density and the P4/E2 ratio in the follicular fluid in vivo. The in vitro gain-of-function experiments revealed that miR-423 suppresses cell apoptosis, especially early apoptosis in GCs. Mechanically speaking, the miR-423 targets and interacts with the 3'-UTR of the porcine SMAD7 gene, which encodes an apoptosis-inducing factor in GCs, and represses its expression and pro-apoptotic function. Interestingly, FA and the GC apoptosis-related lncRNA NORHA was demonstrated as a ceRNA of miR-423. Additionally, we showed that a single base deletion/insertion in the miR-423 promoter is significantly associated with the number of stillbirths (NSB) trait of sows. Conclusion These results demonstrate that miR-423 is a small molecule for inhibiting FA initiation and GC early apoptosis, suggesting that treating with miR-423 may be a novel approach for inhibiting FA initiation and improving female fertility.

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Section: Discussionmentioning

confidence: 99%

miR-423 sponged by lncRNA NORHA inhibits granulosa cell apoptosis

Li,

Zhang,

Wang

et al. 2023

J Animal Sci Biotechnol

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“…As a chief factor of post-transcriptional regulation, miRNA activity can be regulated by lncRNA through "sponge" adsorption. As ceRNA, lncRNA competitively binds to miRNA, affecting the ceRNAinduced gene silencing and thereby regulating the protein level of coding genes and participating in the expression regulation of target genes (26). ceRNA can participate in the pathophysiology of hypertension through various molecular functions (27).…”

Section: Discussionmentioning

confidence: 99%

“…ceRNA can participate in the pathophysiology of hypertension through various molecular functions (27). lncRNA FENDRR plays a positive role in essential hypertension by regulating the miR-423-5p/Nox4 axis, inhibiting cell proliferation and migration and accelerating cell apoptosis, thereby promoting the dysfunction of human umbilical vein endothelial cells (26). However, research on the ceRNA network in Xinjiang Kazakh hypertension has not yet been reported.…”

Section: Discussionmentioning

confidence: 99%

Bioinformatics analysis of lncRNA-related ceRNA networks in the peripheral blood lymphocytes of Kazakh patients with essential hypertension in Xinjiang

Wang

Gao

Zhang

et al. 2023

Front. Cardiovasc. Med.

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ObjectiveHere, we aimed to investigate long non-coding RNA (lncRNA) expression characteristics in the peripheral blood lymphocytes of Xinjiang Kazakh people with essential hypertension and the underlying regulatory mechanisms of competing endogenous RNAs (ceRNA).MethodsFrom April 2016 to May 2019, six Kazakh patients with essential hypertension and six Kazakh healthy participants were randomly selected from the inpatient and outpatient cardiology departments of the First Affiliated Hospital of Shihezi University Medical College, Xinjiang. After detecting the expression levels of lncRNA and mRNA in the peripheral blood lymphocytes using gene chip technology, their levels in the hypertensive group were compared with those in the control group. Six differentially expressed lncRNAs were randomly selected for real-time PCR to verify the accuracy and reliability of the gene chip results. GO functional clustering and KEGG pathway analyses were performed for differentially expressed genes. The ceRNA regulatory network of lncRNA-miRNA-mRNA was constructed, followed by visualization of the results. The expressions of miR-139-5p and DCBLD2 after PVT1 overexpression in 293T cells were detected by qRT-PCR and Western blot.ResultsIn the test group, 396 and 511 differentially expressed lncRNAs and mRNAs, respectively, were screened out. The trend of real-time PCR results was consistent with that of the microarray results. The differentially expressed mRNAs were found to be primarily involved in the adhesion spot, leukocyte migration via endothelial cells, gap junction, actin cytoskeleton regulation, and extracellular matrix-receptor interaction signaling pathways. By constructing the ceRNA regulatory network, we found that lncRNA PVT1-miR-139-5p-DCBLD2 has a potential ceRNA regulatory mechanism involved in the development of essential hypertension in Xinjiang Kazakh people. In 293T cells, lncRNA PVT1 overexpression inhibited miR-139-5p and DCBLD2 levels.ConclusionsOur findings indicate that differentially expressed lncRNAs may be involved in the development of essential hypertension. lncRNA PVT1-miR-139-5p-DCBLD2 was indicated to comprise a potential ceRNA regulatory mechanism involved in the development of essential hypertension in the Xinjiang Kazakh population. Thus, it may act as a novel screening marker or therapeutic target for essential hypertension in this population.

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“…In recent years, the search for ncRNAs has attracted the attention of many researchers. According to reports, increasing studies have shown that lncRNAs have important roles in the occurrence and development of essential hypertension (74)(75)(76)(77)(78). As a special type of lncRNA, the roles of HOX-lncRNAs in essential hypertension have rarely been studied.…”

Section: Involvement Of Hox-lncrnas In Cardiomyocyte Apoptosismentioning

confidence: 99%

Spotlight on HOX cluster‑embedded antisense lncRNAs in cardiovascular diseases (Review)

Zhou,

2023

Int J Mol Med

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Atherosclerosis is a complex and chronic inflammatory disease driven by multiple pathophysiological processes that are responsible for diverse cardiovascular events. Atherosclerotic cardiovascular disease, despite substantial triumphs in primary and secondary prevention, remains a dominant epidemic that impairs human health. Therefore, deciphering the pathogenesis of atherosclerosis will provide a real-world translational understanding. Homeobox cluster-embedded antisense long non-coding RNAs (HOX-lncRNAs), a nascent class of lncRNA molecules with versatile roles in cancer, can also orchestrate various cell functions in cardiovascular disorders and have thus captured the attention of many researchers. Subsequently, numerous studies have demonstrated the role of HOX-lncRNAs as potential modulators of atherosclerosis. Nevertheless, given that the understanding of HOX-lncRNAs in atherosclerosis is only just emerging, ongoing research must be initiated to thoroughly pinpoint such causal roles. The present review aimed to highlight the important contributions of HOX-lncRNAs to atherosclerosis and other pivotal biological processes related to cardiovascular disease. The review concludes with a discussion of the limitations, outlook, challenges and possible solutions associated with HOX-lncRNAs in atherosclerosis. Looking forward, this may lead to extraordinary breakthroughs in revealing the molecular underpinnings of HOX-lncRNAs and may offer a promising yet challenging landscape for robust therapeutic strategies for atherosclerosis and/or associated cardiovascular disorders.

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LncRNA FENDRR Servers as a Possible Marker of Essential Hypertension and Regulates Human Umbilical Vein Endothelial Cells Dysfunction via miR-423-5p/Nox4 Axis

Cited by 7 publications

References 43 publications

miR-423 sponged by lncRNA NORHA inhibits granulosa cell apoptosis

miR-423 sponged by lncRNA NORHA inhibits granulosa cell apoptosis

Bioinformatics analysis of lncRNA-related ceRNA networks in the peripheral blood lymphocytes of Kazakh patients with essential hypertension in Xinjiang

Spotlight on HOX cluster‑embedded antisense lncRNAs in cardiovascular diseases (Review)

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