LMW-i Type Subunits are Expressed in Wheat Endosperm and Belong to the Glutenin Fraction

Ferrante, Paola; Patacchini, C.; Masci, Stefania; D’Ovidio, R.; Lafiandra, D.

doi:10.1039/9781847552099-00136

Cited by 6 publications

(2 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although the first i-type sequence was published in 1988 (Pitts et al 1988 ), the different structure of the i-type genes, compared to m-type, did not receive enough attention: it was thought that these sequences probably belonged to truncated genes. Cloutier et al ( 2001 ) recently reported the gene sequence and characterization of an i-type LMW-GS; expression of i-type LMW-GS genes was proven also by proteomics work (Ferrante et al 2004 ) and by analysing ESTs databases (Juhász et al 2003 ). Juhász and Gianibelli ( 2006 ) assumed that i-type LMW genes are characteristic of the Glu-A3 locus.…”

Section: Discussionmentioning

confidence: 99%

A catalogue of Triticum monococcum genes encoding toxic and immunogenic peptides for celiac disease patients

et al. 2008

View full text Add to dashboard Cite

The celiac disease (CD) is an inXammatory condition characterized by injury to the lining of the smallintestine on exposure to the gluten of wheat, barley and rye. The involvement of gluten in the CD syndrome has been studied in detail in bread wheat, where a set of "toxic" and "immunogenic" peptides has been deWned. For wheat diploid species, information on CD epitopes is poor. In the present paper, we have adopted a genomic approach in order to understand the potential CD danger represented by storage proteins in diploid wheat and sequenced a suYciently large number of cDNA clones related to storage protein genes of Triticum monococcum. Four bona Wde toxic peptides and 13 immunogenic peptides were found. All the classes of storage proteins were shown to contain harmful sequences. The major conclusion is that einkorn has the full potential to induce the CD syndrome, as already evident for polyploid wheats. In addition, a complete overview of the storage protein gene arsenal in T. monococcum is provided, including a full-length HMW x-type sequence and two partial HMW y-type sequences.

show abstract

Section: Discussionmentioning

confidence: 99%

A catalogue of Triticum monococcum genes encoding toxic and immunogenic peptides for celiac disease patients

et al. 2008

View full text Add to dashboard Cite

show abstract

“…Gluten proteins can be divided into gliadins (soluble in a 50 % alcohol solution) and glutenins (insoluble in a 50 % alcohol solution due to formation of inter-chain disulfide bonds and entanglements between longer chains) (Lafiandra et al. 2004 ; Wieser 2007 ). Each of these protein groups comprises many different sequences, over 100 in total (Wieser 2007 ).…”

Section: Methodsmentioning

confidence: 99%

Choosing the right density for a concentrated protein system like gluten in a coarse-grained model

Mioduszewski

2023

Eur Biophys J

View full text Add to dashboard Cite

Large coarse-grained simulations are often conducted with an implicit solvent, which makes it hard to assess the water content of the sample and the effective concentration of the system. Here the number and the size of cavities and entanglements in the system, together with density profiles, are used to asses the homogeneity and interconnectedness of gluten. This is a continuation of an earlier article, "Viscoelastic properties of wheat gluten in a molecular dynamics study" (Mioduszewski and Cieplak 2021b). It turns out there is a wide range of densities (between 1 residue per cubic nanometer and 3 residues/nm$$^3$$ 3 ) where the system is interconnected, but not homogeneous: there are still large empty spaces, surrounded by an entangled protein network. Those findings should be of importance to any coarse-grained simulation of large protein systems.

show abstract

Chapter 6 Low-Molecular-Weight Glutenin Subunits: Insights into this Abundant Subunit Group Present in Glutenin Polymers

Juhász

Gianibelli

2006

Gliadin and Glutenin: The Unique Balance of Wheat Quality

View full text Add to dashboard Cite

LMW-i Type Subunits are Expressed in Wheat Endosperm and Belong to the Glutenin Fraction

Cited by 6 publications

References 0 publications

A catalogue of Triticum monococcum genes encoding toxic and immunogenic peptides for celiac disease patients

A catalogue of Triticum monococcum genes encoding toxic and immunogenic peptides for celiac disease patients

Choosing the right density for a concentrated protein system like gluten in a coarse-grained model

Chapter 6 Low-Molecular-Weight Glutenin Subunits: Insights into this Abundant Subunit Group Present in Glutenin Polymers

Contact Info

Product

Resources

About