Chapter 6 Low-Molecular-Weight Glutenin Subunits: Insights into this Abundant Subunit Group Present in Glutenin Polymers

Novel insights into the composition, variation, organization, and expression of the low-molecular-weight glutenin subunit gene family in common wheat

“…All proteins encoded by the genes identified in the present study were typical of LMW-GS, which had similar structures to previously characterized LMW-GS (D’Ovidio and Masci, 2004; Juhász and Gianibelli, 2006). Each deduced protein contained four main structural domains: a signal peptide, a short conserved N-terminal domain, a repetitive domain, and a C-terminal domain, except for i-type proteins, which lacked the N-terminal domain (Supplementary Fig.…”

“…LMW-GS genes in common wheat are complex, and their exact copy number remains unclear (Cassidy et al ., 1998; Ikeda et al ., 2002; Juhász and Gianibelli, 2006; Huang and Cloutier, 2008; Dong et al ., 2010). Recently, using BAC library screening, 14 and 19 genes were isolated from the common wheat varieties Xiaoyan 54 and Glenlea, respectively (Huang and Cloutier, 2008; Dong et al ., 2010).…”

“…These viscoelastic properties allow dough to be incorporated into a wide range of daily food products, which are affected by glutenin and gliadin proteins in wheat seeds (Shewry et al ., 1995; D’Ovidio and Masci, 2004; Juhász and Gianibelli, 2006). Glutenin proteins are composed of two groups of subunits, namely high-molecular-weight and low-molecular-weight glutenin subunits (HMW-GS, 65–90kDa; LMW-GS, 30–45kDa) (Payne, 1987; D’Ovidio and Masci, 2004).…”

“…The LMW-GS account for about one-third of the seed protein and 60% of glutenin proteins, and play an important role in determining dough properties and the quality of wheat food products (Gupta et al ., 1991, 1994; Branlard et al ., 2001; Eagles et al ., 2002; Howitt et al ., 2006). Thus, elucidating the composition and variation of LMW-GS genes in common wheat and investigating the relationship between allelic variants and end-use quality are of interest for wheat quality improvement (Gupta et al ., 1994; He et al ., 2005; Bekes et al ., 2006; Juhász and Gianibelli, 2006; Liu et al ., 2010; Zhang et al ., 2012). …”

“…The copy number of LMW-GS genes was estimated to range from 10–20 to 30–40 (Ikeda et al ., 2002; D’Ovidio and Masci, 2004; Juhász and Gianibelli, 2006; Huang and Cloutier, 2008; Dong et al ., 2010; Zhang et al ., 2011 a , b ). In Norin 61, 12 groups of LMW-GS genes were identified by screening the cDNA library (Ikeda et al ., 2006).…”