Lithium plus pilocarpine induced status epilepticus — biochemical changes

Eraković, Vesna; Župan, Gordana; Varljen, Jadranka; Laginja, Josip; Simonić, Ante

doi:10.1016/s0168-0102(99)00120-0

Cited by 73 publications

(32 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It demonstrated permanent changes in different biochemical systems during status epilepticus. An increase in lipid peroxidation and excessive free radical formation occur during status epilepticus induced by pilocarpine (Erakovie et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Assessment of effect of ethanolic extract of Tephrosia purpurea (L.) Pers., Fabaceae, activity on lithium-pilocarpine induced Status epilepticus and oxidative stress in Wistar rats

Asuntha¹,

Prasannaraju²,

Sujatha³

et al. 2010

Rev. bras. farmacogn.

View full text Add to dashboard Cite

RESUMO: "Avaliação do efeito do extrato etanólico da Tephrosia purpurea (L.) Pers., Fabaceae, sobre o status epilepticus induzido por lítio-pilocarpina e estresse oxidativo, em ratos Wistar". Tephrosia purpurea (L.) Pers., Fabaceae, é conhecida pelo seu uso no controle e tratamento de uma variedade de distúrbios epilépticos no sistema indiano de medicina. O presente estudo pretende avaliar de forma sistemática T. purpurea e verificar essa alegação. ABSTRACT: Tephrosia purpurea (L.) Pers., Fabaceae, is claimed to be of use in the control and treatment of a variety of epileptic disorders in Indian system of medicine. The present study plans to systematically evaluate T. purpurea and to verify this claim. Status epilepticus was induced in male albino rats of Wistar strain by administration of pilocarpine (30 mg/kg, i.p.) 24 h after lithium chloride (3 mEq/kg, i.p.). Different doses of the extract of T. purpurea were administered orally one hour before the injection of pilocarpine. The severity of status epilepticus was observed and recorded every 15 min till 90 min and thereafter every 30 min till 180 min, using the scoring system. The in vivo lipid peroxidation of rat brain tissue was measured. The in vitro NO free radical scavenging activity of plant extract was assessed. The interaction between plant extract and 2-diphenyl-2-picryl hydrazyl (DPPH) was also observed for in vitro free radical scavenging activity. The severity of status epilepticus was reduced with the administration of ethanol extract of T. purpurea. Ethanol extract of the plant exhibited both in vivo and in vitro antioxidant activity. The ethanolic extract of T. purpurea was found to be useful to control lithium-pilocarpine induced status epilepticus in albino rats of Wistar strain.

show abstract

Section: Introductionmentioning

confidence: 99%

Assessment of effect of ethanolic extract of Tephrosia purpurea (L.) Pers., Fabaceae, activity on lithium-pilocarpine induced Status epilepticus and oxidative stress in Wistar rats

Asuntha¹,

Prasannaraju²,

Sujatha³

et al. 2010

Rev. bras. farmacogn.

View full text Add to dashboard Cite

show abstract

“…A ativação do receptor colinérgico muscarínico (Persinger et al, 1993;Marinho et al, 1998) foi sugerida como responsável pelas convulsões produzidas pela pilocarpina, dando a entender que após ativação do sistema colinérgico haveria uma interação direta e/ou indireta com outros sistemas, a saber: dopaminérgico (Al-Tajir et al, 1990a;Barone et al, 1991), glutamatérgico (Fujikawa et al, 1994(Fujikawa et al, e 1995 e GABAérgico (Fritschy et al, 1999;Erakovic et al, 2000;CostaLotufo et al, 2002), que podem ser ativados para a manutenção e/ou propagação das convulsões. Contudo, os neurotransmissores e as estruturas cerebrais envolvidas na convulsão precisam ser prontamente esclarecidos, a fim de contribuir para a compreensão da gênese do estado epiléptico, bem como investigar novos agentes terapêuticos.…”

Section: Introductionunclassified

“…O estudo do cérebro desenvolvido, com suas inervações e densidade de receptores operantes, favorece o conhecimento da epilepsia em relação ao estudo das convulsões em animais adultos (George e Kulkarni, 1996), uma vez que pode ajudar na investigação dos mecanismos de manutenção e propagação das convulsões, ainda ausentes em ratos jovens, podendo ainda indicar as possíveis alterações nas concentrações dos neurotransmissores (Erakovic et al, 2000) que ocorreram no estriado maduro durante o estado epiléptico.…”

Section: Introductionunclassified

Níveis dos neurotransmissores estriatais durante o estado epiléptico

Freitas¹,

Viana²,

Fonteles³

2003

Rev. psiquiatr. clín.

View full text Add to dashboard Cite

RESUMOO objetivo desse estudo foi verificar os níveis dos neurotransmissores estriatais de ratas adultas durante o estado epiléptico induzido pela pilocarpina. Ratas wistar foram tratadas com uma única dose de pilocarpina (400 mg/kg por via subcutânea (S.C.); P400) e os controles receberam salina. A concentração dos neurotransmissores foi determinada através do HPLC eletroquímico, no corpo estriado de ratas que no período de observação de 1 hora desencadearam estado epiléptico e que sobreviveram à fase aguda do quadro convulsivo. Foi observada redução nos níveis de dopamina, serotonina, ácido diidroxifenilacético e aumento na concentração do ácido 5-hidroxiindolacético. Nenhuma alteração foi observada no 4-hidroxi-3-metoxi-fenilacético. Os resultados sugerem que a ativação do sistema colinérgico pode interagir com os sistemas dopaminérgico e serotonérgico nos mecanismos referentes à fase aguda do processo convulsivo no corpo estriado de ratos desenvolvidos.Unitermos: Dopamina; Serotonina; Convulsão; Corpo estriado. ABSTRACT Striatal monoamines levels during status epilepticusThe purpose of the present work to investigate the striatal neurotransmissors level in adult rats after status epilepticus induced by pilocarpine. Wistar rats were treated with a single dose of pilocarpine (400 mg/kg; s.c.; P400) and the controls received saline. Adult animals were closed observed for behavioural changes during 1h. In this period, the animals that developed status epilepticus and survive this acute phase of seizures had the brains removed and striatal neurotransmissors level determiden by HPLC. The concentration of dopamine, serotonine, dihydroxyphenylacetic acid was reduced and an concentration increase in 5-hydroxyindolacetic acid. Didn't observed alteration in 4-hydroxy-3-methoxy-phenylacetic acid. These results suggest that cholinergic activation can interage with dopaminergic and serotonergic systems in acute phase of the convulsive process in rat mature striatum.

show abstract

“…In a recent study we have demonstrated that lithium plus pilocarpine (LiPi) administration and the resulting status epilepticus bring about a significant increase in the rat brain FFA content and a decrease in glutathione peroxidase (GPX) activity in the frontal cortex, cerebellum and hippocampus [9]. Since LiPi-induced status epilepticus is initiated by cholinergic overstimulation and propagated by glutaminergic neurotransmission, subsequent brain damage may be caused by overactive NMDA receptor-mediated mechanism [ 161.…”

Section: Introductionmentioning

confidence: 99%

The influence of calcium channel blockers on the brain free fatty acid level and glutathione peroxidase activity in rats with lithium and pilocarpine‐induced status epilepticus

Eraković¹,

Župan²,

Varljen³

et al. 2002

Neuroscience Res Communica

Self Cite

View full text Add to dashboard Cite

The aim of this study was to investigate possible therapeutic approach to neuronal damage caused by status epilepticus. The effects of the L-type voltage-sensitive calcium channel blockers (nimodipine, nicardipine) and NMDA blockers (ifenprodil and MK-801) on the brain free fatty acid (FFA) level and glutathione peroxidase (GPX) activity in rats with lithium and pilocarpine (LiPi) -induced status epilepticus were examined. Vehicle or various doses of calcium channel and NMDA blockers had been injected i.p. 30 min after the pilocarpine application. Tested doses of nicardipine and MK-801 did not influence the increase in the brain FFA level. Nimodipine and ifenprodil revealed a tendency to reduce FFA level. The inhibition of L-type voltage-sensitive Ca2' channels or NMDA receptor -associated channels was not sufficient for significant attenuation of an increase in the rat brain FFA content and a decrease GPX activity associated with LiPi -induced status epilepticus. Individual compounds have shown to prevent FFA increase, suggesting some alternative or additional mechanism of action. The prevention of FFA accumulation was not followed by the prevention of GPX decrease indicating the activation of non FFA-related mechanisms of cell damage.

show abstract

Lithium plus pilocarpine induced status epilepticus — biochemical changes

Cited by 73 publications

References 49 publications

Assessment of effect of ethanolic extract of Tephrosia purpurea (L.) Pers., Fabaceae, activity on lithium-pilocarpine induced Status epilepticus and oxidative stress in Wistar rats

Assessment of effect of ethanolic extract of Tephrosia purpurea (L.) Pers., Fabaceae, activity on lithium-pilocarpine induced Status epilepticus and oxidative stress in Wistar rats

Níveis dos neurotransmissores estriatais durante o estado epiléptico

The influence of calcium channel blockers on the brain free fatty acid level and glutathione peroxidase activity in rats with lithium and pilocarpine‐induced status epilepticus

Contact Info

Product

Resources

About