Listeria monocytogenes in Raw Milk: Detection, Incidence, and Pathogenicity

Lovett, Joseph; Francis, David; Hunt, Jan M.

doi:10.4315/0362-028x-50.3.188

Cited by 297 publications

(144 citation statements)

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“…After 24 and 72 h incubation 10 ml of the buffered peptone water was added to 30 ml of listeria enrichment broth [9]. The listeria enrichment broth was subcultured onto acriflavine-ceftazidime agar (CM856, Oxoid) and incubated further for 24 h at 30 'C.…”

Section: Methodsmentioning

confidence: 99%

The isolation ofListeriaspecies from fresh-water sites in Cheshire and North Wales

Frances¹,

Hornby²,

Hunter³

1991

Epidemiol. Infect.

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SUMMARYWe report a study to determine the presence of Listeria species in surface waters. One hundred ml volumes of 30 water samples taken from 21 different sites were analysed. Most of the samples examined were from ponds and lakes. Listeria species were isolated on eight (27%) occasions, six of these isolates were L. seeligeri, one was L. innocua and one L. welshimeri. Although not statistically significant, coliform and Escherichia coli counts were higher in waters that were positive than were negative for Listeria spp. It is suggested that the low isolation rate of listeria in this study reflects the fact that most waters examined did not receive sewage outfalls. Water sports activities are unlikely to be a risk factor for listeriosis.

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Section: Methodsmentioning

confidence: 99%

The isolation ofListeriaspecies from fresh-water sites in Cheshire and North Wales

Frances¹,

Hornby²,

Hunter³

1991

Epidemiol. Infect.

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“…The same study was carried out in Spain in which 67 samples, but the prevalence rate 44.7 % very high for listeria monocytogenes by Garayzabal et al, (1987). In USA, another study was carried out by Lovett et al, (1987) which showed high occurrence of listeria monocytogenes because 200 samples were observed in which 14 were positive for listeria monocytogenes. Harvey et al, (1992) studied 176 samples in which prevalence is 15.3 % for Listeria monocytogenes.…”

Section: Discussionmentioning

confidence: 99%

Prevalence and antimicrobial resistance of Listeria monocytogenes isolated from raw milk and dairy products

Gohar¹,

Abbas²,

sajid³

et al. 2017

Matrix sci. medica

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Listeriosis is a severe foodborne disease commonly caused by eating contaminated food with the Listeria species. A large variety of foods, especially dairy foods and ready-to-eat products, can support the growth of pathogens. Outbreaks of listeriosis have been related with milk, cheese, vegetable salads, and meat products, and fatality rates are typically around 20% due to listeriosis. The Centers for Disease Control and Prevention evaluates that 2500 infections and more than 500 deaths are related with listeriosis each year in the United States. A total 125 milk and dairy products were included in the study. Isolation and identification of this specie was done and then confirmed it by gram staining. Antimicrobial sensitivity was also checked. Prevalence of Listeria species were 16.8%, Listeria monocytogenes was 13.6%. Listeria monocytogenes was resistant against Ampicillin, Amoxicillin, Penicillin and sensitive Fosfomycin, Ciprofloxacin, Gentamycin. The results of this study showed the low prevalence of Listeria monocytogenes.

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“…This organism has been isolated from silage [2], natural vegetation [3], soil [4], wild and domestic animals [5] and water [6]. It also is reported to occur in foods such as raw milk [7], soft cheeses [8], raw chickens [9], pork meat [10], and minced beef [11].…”

Section: Introductionmentioning

confidence: 99%

A comparative study of clinical and food isolates ofListeria monocytogenesand related species

Szabo

Desmarchelier

1990

Epidemiol. Infect.

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SUMMARYNinety-six isolates of presumptive or confirmed Listeria monocytogenes were obtained from local clinical (30 isolates) or food laboratories (66 isolates). Minimal biochemical analysis identified only 80 % of these isolates as L. monocytogenes the remaining included L. seeligeri, 1 %, or the non-haemolytic L. innocua, 19 %. The 27 clinical and 50 food isolates, mainly from meat products, frozen confectionaries, and cheeses, confirmed as L. monocytogenes were compared biochemically and serologically. Twenty-one isolates, including some strains of L. innocua and L. seeligeri, were examined for pathogenicity in immunocompromized mice and 44 typed using bacterial restriction endonuclease DNA analysis (BRENDA). Only isolates of L. monocytogenes were found to be pathogenic. Biovar-typing of the isolates was unreliable and provided poor discrimination. Serogroups 1/2 and 4 predominated among clinical and food isolates and BRENDA provided better discrimination among isolates. Ten stable and reproducible restriction patterns were observed among the Listeria sp. isolates studied. Overall, a combination of techniques gave the best discrimination and indicated their potential for use as epidemiological tools.

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Listeria monocytogenes in Raw Milk: Detection, Incidence, and Pathogenicity

Cited by 297 publications

References 0 publications

The isolation ofListeriaspecies from fresh-water sites in Cheshire and North Wales

The isolation ofListeriaspecies from fresh-water sites in Cheshire and North Wales

Prevalence and antimicrobial resistance of Listeria monocytogenes isolated from raw milk and dairy products

A comparative study of clinical and food isolates ofListeria monocytogenesand related species

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