Lirioresinol B dimethyl ether inhibits NF-κB and COX-2 and activates IκBα expression in CCl4-induced hepatic fibrosis

Shehzad, Adeeb; Rehmat, Shagufta; Ul-Islam, Salman; Ahmad, Rizwan; Aljafary, Meneerah Abdulrahman; Alrushaid, Noor; Al-Suhaimi, Ebtesam A.

doi:10.1186/s12906-020-2839-3

Cited by 6 publications

(3 citation statements)

References 42 publications

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“…Fraction E also provides cytotoxic activity, although it was not efficacious than fraction B. This activity might be affected by NF- κ B signaling pathway inhibition [ 31 ].…”

Section: Resultsmentioning

confidence: 99%

Cytotoxic and Antimigration Activity of Etlingera alba (A.D.) Poulsen Rhizome

Wahyuni

Diantini

Ghozali

et al. 2021

Advances in Pharmacological and Pharmaceutical Sciences

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Etlingera alba is one of the Etlingera plants that might have anticancer activity. This study aims to investigate the cytotoxic and antimetastatic activity of E. alba rhizome fractions and migration cell assay against MDA-MB-231 cell lines, which are used for triple-negative breast cancer (TNBC) treatment assay. The cytotoxic activity was assayed using CCK-8 assay, while the antimetastatic was assayed using migration cell assay for the fractions A–F. They were followed by LCMS/MS profiling to determine the chemical contents in the most active fraction. According to results obtained, fraction B was the most active fraction for cytotoxic activity with an IC50 value of 65.43 μg/mL, while fraction E was the most active fraction for antimetastasis activity against migration rate doses of 50, 100, and 200 ppm which were 6.80, 3.66, and 3.00%, respectively. Several compounds in fraction B, such as rengyolone, licochalcone A, sugiol, and spinasterol, might have been known to have activity against cancer cells, as well as aschantin and lirioresinol B dimethyl ether from fraction E. In conclusion, the chemical components from E. alba rhizome fractions provided potency for discovering new agents for cancer treatment, specifically for TNBC.

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“…Fraction E also provides cytotoxic activity, although it was not efficacious than fraction B. This activity might be affected by NF- κ B signaling pathway inhibition [ 31 ].…”

Section: Resultsmentioning

confidence: 99%

Cytotoxic and Antimigration Activity of Etlingera alba (A.D.) Poulsen Rhizome

Wahyuni

Diantini

Ghozali

et al. 2021

Advances in Pharmacological and Pharmaceutical Sciences

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“…The current investigation showed that the pro-inflammatory signaling of NF-κB activation and COX2 were significantly upregulated in SHR rats. NF-κB regulates inflammatory cytokine production via the COX2 pathway, which is critical in mediating fibrosis [ 38 , 39 ]. Stress-induced cardiac remodeling (physiological and pathological hypertrophy), permanent functional impairment, and heart failure are the consequences of prolonged cellular adaptation [ 3 ].…”

Section: Discussionmentioning

confidence: 99%

Dipeptide IF and Exercise Training Attenuate Hypertension in SHR Rats by Inhibiting Fibrosis and Hypertrophy and Activating AMPKα1, SIRT1, and PGC1α

Baskaran

Wang

et al. 2022

IJMS

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Bioactive peptides are physiologically active peptides produced from proteins by gastrointestinal digestion, fermentation, or hydrolysis by proteolytic enzymes. Bioactive peptides are resorbed in their whole form and have a preventive effect against various disease conditions, including hypertension, dyslipidemia, inflammation, and oxidative stress. In recent years, there has been a growing body of evidence showing that physiologically active peptides may have a function in sports nutrition. The present study aimed to evaluate the synergistic effect of dipeptide (IF) from alcalase potato protein hydrolysates and exercise training in hypertensive (SHR) rats. Animals were divided into five groups. Bioactive peptide IF and swimming exercise training normalized the blood pressure and decreased the heart weight. Cardiac, hepatic, and renal functional markers also normalized in SHR rats. The combined administration of IF peptide and exercise offer better protection in SHR rats by downregulating proteins associated with myocardial fibrosis, hypertrophy, and inflammation. Remarkably, peptide treatment alongside exercise activates the PI3K/AKT cell survival pathway in the myocardial tissue of SHR animals. Further, the mitochondrial biogenesis pathway (AMPKα1, SIRT1, and PGC1α) was synergistically activated by the combinatorial treatment of IF and exercise. Exercise training along with IF administration could be a possible approach to alleviating hypertension.

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“…Evidence shows that CCl 4 toxicity can trigger the recruitment inflammatory mediators, such as TNF- α , IL-1 β , IL-6, Cox2, and iNOS, thereby inducing liver inflammation [ 46 ]. TNF- α , IL-1 β , and IL-6 mainly contribute to HSCs activation; Cox2 and iNOS are the main regulators of the inflammatory response; Cox2 induces prostaglandin E2 (PGE2) production and then mediates inflammation in the liver disease models [ 47 ]; the continuous production of NO is associated with the increased iNOS expression. In our experiment, HJRG downregulated the expression of the proinflammatory factors (TNF- α , IL-1 β , and IL-6).…”

Section: Discussionmentioning

confidence: 99%

Huangjia Ruangan Granule Inhibits Inflammation in a Rat Model with Liver Fibrosis by Regulating TNF/MAPK and NF-κB Signaling Pathways

Cai

Wang²,

Zhang

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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The Huangjia Ruangan granule (HJRG) is a clinically effective Kampo formula, which has a significant effect on liver fibrosis and early liver cirrhosis. However, the mechanism underlying HJRG in treating liver fibrosis remains unclear. In this study, carbon tetrachloride (CCl4) was used to induce liver fibrosis in rats to clarify the effect of HJRG on liver fibrosis and its mechanism. Using network pharmacology, the potential mechanism of HJRG was initially explored, and a variety of analyses were performed to verify this mechanism. In the liver fibrosis model, treatment with HJRG can maintain the liver morphology, lower the levels of AST and ALT in the serum, and ameliorate pathological damage. Histopathological examinations revealed that the liver structure was significantly improved and fibrotic changes were alleviated. It can effectively inhibit collagen deposition and the expression of α-SMA, reduce the levels of the rat serum (HA, LN, PC III, and Col IV), and inhibit the expression of desmin, vimentin, and HYP content in the liver. Analyzing the results of network pharmacology, the oxidative stress, inflammation, and the related pathways (primarily the TNF signaling pathway) were identified as the potential mechanism of HJRG against liver fibrosis. Experiments confirmed that HJRG can significantly increase the content of superoxide dismutase and glutathione and reduce the levels of malondialdehyde and myeloperoxidase in the rat liver; in addition, HJRG significantly inhibited the content of proinflammatory cytokines (TNF-α, IL-1β, and IL-6) and reduced the expression of inflammatory regulators (Cox2 and iNOS). Meanwhile, treatment with HJRG inhibited the phosphorylation of NF-κB P65, IκBα, ERK, JNK, and MAPK P38. Moreover, HJRG treatment reversed the increased expression of TNFR1. The Huangjia Ruangan granule can effectively inhibit liver fibrosis through antioxidation, suppressing liver inflammation by regulating the TNF/MAPK and NF-κB signaling pathways, thereby preventing the effect of liver fibrosis.

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Lirioresinol B dimethyl ether inhibits NF-κB and COX-2 and activates IκBα expression in CCl4-induced hepatic fibrosis

Cited by 6 publications

References 42 publications

Cytotoxic and Antimigration Activity of Etlingera alba (A.D.) Poulsen Rhizome

Cytotoxic and Antimigration Activity of Etlingera alba (A.D.) Poulsen Rhizome

Dipeptide IF and Exercise Training Attenuate Hypertension in SHR Rats by Inhibiting Fibrosis and Hypertrophy and Activating AMPKα1, SIRT1, and PGC1α

Huangjia Ruangan Granule Inhibits Inflammation in a Rat Model with Liver Fibrosis by Regulating TNF/MAPK and NF-κB Signaling Pathways

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