Liquid separation techniques coupled with mass spectrometry for chiral analysis of pharmaceuticals compounds and their metabolites in biological fluids

Abstract

Determination of the chiral composition of drugs is nowadays a key step in order to determine purity, activity, bioavailability, biodegradation, etc, of pharmaceuticals. In this manuscript, works published for the last 5 years on the analysis of chiral drugs by liquid separation techniques coupled with mass spectrometry are reviewed. Namely, chiral analysis of pharmaceuticals including e.g., antiinflammatories, antihypertensives, relaxants, etc, by liquid chromatography-mass spectrometry and capillary electrophoresis-mass spectrometry are included. The importance and interest of the analysis of the enantiomers of the active compound and its metabolites in different biological fluids (plasma, urine, cerebrospinal fluid, etc) are also discussed.

Sensitive chiral analysis by CE: An update

“…This hyphenation enables to obtain information on the solute's molecular weight and structure and may enhance the sensitivity of certain compounds depending on their characteristics (proton affinity, absorptivity, etc.). In the last 10 years, there were many significant developments in CE-MS instrumentation and applications that have made CE-MS a competitive tool [9,52].…”

“…Some reviews have been published in the last years covering the chiral separation of drugs [8][9][10] or pollutants and their metabolites [11]. The aim of this review is to provide a general view of the different strategies that have been used in the last years in order to enhance the sensitivity of detection in chiral analysis by CE with application to the analysis of drugs or biological, environmental, or food samples.…”

Review of aqueous chiral electrokinetic chromatography (EKC) with an emphasis on chiral microemulsion EKC

“…Due to the vast exploration in this subject, numerous reviews detailing fundamentals and applications can be found in the literature prior to 2005 [6][7][8][9][10][11] and over the past two years [12][13][14][15]. Reviews have also covered methods developed specifically for pharmaceutical compounds [16][17][18][19][20][21][22], biological compounds [23], chiral LC-MS/ CE-MS [24], pesticides [25], pollutants [26], and food additives [27,28].…”

Recent advances in the application of capillary electromigration methods for food analysis

“…In addition, some other published reviews were focused on discussing the different applications of a particular CE technique, including food analysis. This is the case for instance of CE coupled to mass spectrometry (MS) [15][16][17], electrochromatography [18], CE coupled to chemiluminiscence detection [19], microemulsion electrokinetic chromatography (MEEKC) [20], sample treatment prior to CE-MS [21].…”