Sensitive chiral analysis by CE: An update

Sánchez‐Hernández, Laura; Crego, Antonio L.; Marina, Marı́a Luisa; García-Ruiz, Carmen

doi:10.1002/elps.200700531

Cited by 53 publications

(48 citation statements)

References 53 publications

(102 reference statements)

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“…For chiral CE, off-line (before injection) or on-line (after injection) preconcentration techniques are widely used to improve the detection sensitivity [14]. Regarding the off-line preconcentration techniques, microextraction techniques, such as liquid-phase microextraction [15][16][17][18] and solid-phase microextraction [19][20][21] were introduced in addition to the conventional LLE and SPE.…”

Section: Introductionmentioning

confidence: 99%

Direct chiral analysis of primary amine drugs in human urine by single drop microextraction in‐line coupled to CE

et al. 2009

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Three-phase single drop microextraction (SDME) was in-line coupled to chiral CE of weakly basic amine compounds including amphetamine. SDME was used for the matrix isolation and sample preconcentration in order to directly analyze urine samples with the minimal pretreatment of adding NaOH. A small drop of an acidic aqueous acceptor phase covered with a thin layer of octanol was formed at the tip of a capillary by simple manipulation of the liquid handling functions of a commercial CE instrument. While the saline matrix of the urine sample was blocked by the octanol layer, the basic analytes in a basic aqueous donor phase were concentrated into the acidic acceptor drop through the octanol layer by the driving force of the pH difference between the two aqueous phases. The enantiomers of the enriched amines were resolved by using (+)-(18-crown-6)-tetracarboxylic acid as a chiral selector for the subsequent CE separation. From 10 min SDME with the agitation of the donor phase by a small stirrer retrofit to the CE instrument, enrichment factors were about a 1000-fold, yielding the LOD of 0.5 ng/mL for amphetamine. This low LOD value as well as the convenience of in-line coupled SDME make the proposed scheme well suited for the demanding chiral analysis of amphetamine-type stimulants.

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Section: Introductionmentioning

confidence: 99%

Direct chiral analysis of primary amine drugs in human urine by single drop microextraction in‐line coupled to CE

et al. 2009

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“…[39][40][41] A teicoplanin based chiral stationary phase was used for the separation of stereoisomers of dinuclear ruthenium(II) complexes and enantiomers of tris(diimine)ruthenium(II) complexes. 42,43 Many reviews [44][45][46][47][48][49][50][51][52][53] have been published on the extensive use of chiral capillary electrophoresis (CE) in recently years. CE has been used for the enantioseparation of pharmaceutical compounds, [50][51][52][53] biological samples, 44 and asymmetric synthesis products and intermediates.…”

Section: Introductionmentioning

confidence: 99%

Enantiomeric separation of chiral ruthenium(II) complexes using capillary electrophoresis

et al. 2008

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Capillary zone electrophoresis (CZE) and micellar capillary electrophoresis (MCE) were applied for the enantiomeric separation of nine mononuclear tris(diimine)ruthenium(II) complexes as well as the separation of all stereoisomers of a dinuclear tris(diimine)ruthenium(II) complex. Nine cyclodextrin (CD) based chiral selectors were examined as run buffer additives to evaluate their effectiveness in the enantiomeric separation of tris(diimine)ruthenium(II) complexes. Seven showed enantioselectivity. Sulfated gamma-cyclodextrin (SGC), with four baseline and three partial separations, was found to be the most useful chiral selector. In CZE mode, the derivatized gamma-CDs were more effective than beta-CDs while sulfated CDs work better than carboxymethyl CDs. In MCE mode, hydroxypropyl beta-CD separated the greatest number of tris(diimine) ruthenium(II) complexes. The effects of chiral selector concentration, run buffer pH and concentration, the concentration ratio between chiral selector and other factors were investigated.

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“…1,[12][13][14][15][16] Most of these methods have been established for the simple and rapid determination of various amino acid enantiomers, and these methods are useful for analyzing relatively large amounts of D-amino acids, such as D-Ser and D-Asp, in mammalian tissues. As described above, a large amount of D-Ser (ca.…”

Section: Two-dimensional Hplc Determination Of Small Amounts Of D-amimentioning

confidence: 99%

Analysis of Small Amounts of d-Amino Acids and the Study of Their Physiological Functions in Mammals

et al. 2009

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D-Amino acids, the enantiomers of L-amino acids, are candidates to be novel physiologically active substances and the biomarkers in mammals. However, the amounts of D-amino acids in the tissues and physiological fluids are extremely small in most cases, and sensitive and selective analytical methods are needed for their determination. In the present manuscript, we review the analytical technologies including our recent advances for the determinations of small amounts of D-amino acids in mammals and the applications to clarify their physiological meanings.

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Sensitive chiral analysis by CE: An update

Cited by 53 publications

References 53 publications

Direct chiral analysis of primary amine drugs in human urine by single drop microextraction in‐line coupled to CE

Direct chiral analysis of primary amine drugs in human urine by single drop microextraction in‐line coupled to CE

Enantiomeric separation of chiral ruthenium(II) complexes using capillary electrophoresis

Analysis of Small Amounts of d-Amino Acids and the Study of Their Physiological Functions in Mammals

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